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学科主题: 药学
题名:
Association of Oxidative Stress with Realgar-Induced Differentiation in Human Leukemia HL-60 Cells
作者: Wang, Li-Wen2; Shi, Yan-Ling2; Wang, Nan1; Gou, Bao-Di1; Zhang, Tian-Lan1,2; Wang, Kui1
关键词: Differentiation ; HL-60 ; Mitochondrion transmembrane potential ; Oxidative stress ; Realgar
刊名: CHEMOTHERAPY
发表日期: 2009
DOI: 10.1159/000265528
卷: 55, 期:6, 页:460-467
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Pharmacology & Pharmacy
研究领域[WOS]: Oncology ; Pharmacology & Pharmacy
关键词[WOS]: ACUTE PROMYELOCYTIC LEUKEMIA ; MITOCHONDRIAL PATHWAY ; HUMAN KERATINOCYTES ; HYDROGEN-PEROXIDE ; INDUCED APOPTOSIS ; NADPH OXIDASE ; INVOLVEMENT ; PROTEIN ; GLUTATHIONE ; CATALASE
英文摘要:

Background: Realgar (arsenic sulfide, As 4 S 4) has been shown to have clinical efficacy in patients with newly diagnosed and relapsed acute promyelocytic leukemia. Mechanistic studies have demonstrated that realgar is able to induce cell differentiation. Methods: The oxidative stress in the realgar-induced differentiation was examined with human leukemia HL-60 cells. Cell differentiation was evaluated by the expression of cell surface antigen CD11b and nitroblue tetrazolium assay. The activities of catalase and superoxide dismutase were measured spectrophotometrically. Flow cytometry was used to assess cell cycle distribution and apoptosis, the cellular level of reactive oxygen species (ROS) and glutathione, as well as mitochondrial transmembrane potential (MTP). Results: The realgar-induced differentiation was enhanced by hydrogen peroxide, and preceded with drastic changes in ROS and catalase, as well as small changes in superoxide dismutase and the reduced form of glutathione. MTP values at 24 h were in linear proportion to the CD11b expression at 48 h when no apoptosis was observed. Conclusion: Oxidative stress and stress-related MTP decrease are associated with realgar-induced differentiation in HL-60 cells. Copyright (C) 2009 S. Karger AG, Basel

语种: 英语
项目资助者: Beijing Municipal Education Commission ; Beijing Talent Project
WOS记录号: WOS:000272605600011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66250
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Dept Biol Chem, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Capital Med Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing, Peoples R China

Recommended Citation:
Wang, Li-Wen,Shi, Yan-Ling,Wang, Nan,et al. Association of Oxidative Stress with Realgar-Induced Differentiation in Human Leukemia HL-60 Cells[J]. CHEMOTHERAPY,2009,55(6):460-467.
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