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Association of Oxidative Stress with Realgar-Induced Differentiation in Human Leukemia HL-60 Cells
Wang, Li-Wen2; Shi, Yan-Ling2; Wang, Nan1; Gou, Bao-Di1; Zhang, Tian-Lan1,2; Wang, Kui1
关键词Differentiation HL-60 Mitochondrion transmembrane potential Oxidative stress Realgar
刊名CHEMOTHERAPY
2009
DOI10.1159/000265528
55期:6页:460-467
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Pharmacology & Pharmacy
研究领域[WOS]Oncology ; Pharmacology & Pharmacy
关键词[WOS]ACUTE PROMYELOCYTIC LEUKEMIA ; MITOCHONDRIAL PATHWAY ; HUMAN KERATINOCYTES ; HYDROGEN-PEROXIDE ; INDUCED APOPTOSIS ; NADPH OXIDASE ; INVOLVEMENT ; PROTEIN ; GLUTATHIONE ; CATALASE
英文摘要

Background: Realgar (arsenic sulfide, As 4 S 4) has been shown to have clinical efficacy in patients with newly diagnosed and relapsed acute promyelocytic leukemia. Mechanistic studies have demonstrated that realgar is able to induce cell differentiation. Methods: The oxidative stress in the realgar-induced differentiation was examined with human leukemia HL-60 cells. Cell differentiation was evaluated by the expression of cell surface antigen CD11b and nitroblue tetrazolium assay. The activities of catalase and superoxide dismutase were measured spectrophotometrically. Flow cytometry was used to assess cell cycle distribution and apoptosis, the cellular level of reactive oxygen species (ROS) and glutathione, as well as mitochondrial transmembrane potential (MTP). Results: The realgar-induced differentiation was enhanced by hydrogen peroxide, and preceded with drastic changes in ROS and catalase, as well as small changes in superoxide dismutase and the reduced form of glutathione. MTP values at 24 h were in linear proportion to the CD11b expression at 48 h when no apoptosis was observed. Conclusion: Oxidative stress and stress-related MTP decrease are associated with realgar-induced differentiation in HL-60 cells. Copyright (C) 2009 S. Karger AG, Basel

语种英语
WOS记录号WOS:000272605600011
资助机构Beijing Municipal Education Commission ; Beijing Talent Project
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66250
专题北京大学药学院_化学生物学系
作者单位1.Peking Univ, Dept Biol Chem, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Capital Med Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Wang, Li-Wen,Shi, Yan-Ling,Wang, Nan,et al. Association of Oxidative Stress with Realgar-Induced Differentiation in Human Leukemia HL-60 Cells[J]. CHEMOTHERAPY,2009,55(6):460-467.
APA Wang, Li-Wen,Shi, Yan-Ling,Wang, Nan,Gou, Bao-Di,Zhang, Tian-Lan,&Wang, Kui.(2009).Association of Oxidative Stress with Realgar-Induced Differentiation in Human Leukemia HL-60 Cells.CHEMOTHERAPY,55(6),460-467.
MLA Wang, Li-Wen,et al."Association of Oxidative Stress with Realgar-Induced Differentiation in Human Leukemia HL-60 Cells".CHEMOTHERAPY 55.6(2009):460-467.
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