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学科主题: 临床医学
题名:
Biological Characteristics of Mesenchymal Stem Cells Grown on Different Topographical Nanofibrous Poly-L-Lactide Meshes
作者: Zhu, Jingxian1; Cai, Qing3; Zhang, Xin1; Hu, Xiaoqing1; Li, La1; Wang, Weiping2; Shao, Zhenxing1; Dai, Linghui1; Cheng, Liyuan3; Yang, Xiaoping3; Zhou, Chunyan2; Ao, Yingfang1
关键词: Mesenchymal Stem Cell ; Extracellular Matrix ; Nanotopography ; Osteogenesis ; Signaling Pathway
刊名: JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
发表日期: 2013-10-01
DOI: 10.1166/jbn.2013.1661
卷: 9, 期:10, 页:1757-1767
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Nanoscience & Nanotechnology ; Materials Science, Biomaterials
研究领域[WOS]: Science & Technology - Other Topics ; Materials Science
关键词[WOS]: OSTEOGENIC DIFFERENTIATION ; EXTRACELLULAR-MATRIX ; CYTOSKELETAL TENSION ; TISSUE ; SCAFFOLDS ; ADHESION ; SURFACES ; ENVIRONMENTS ; FIBRONECTIN ; OSTEOBLASTS
英文摘要:

The nanotopographical features of artificial scaffolds have complex effects on the biological characteristics of stem cells. They influence cell adhesion, spreading, proliferation, and differentiation; however we have limited knowledge on how these processes occur under nanotopographical cues. In this study, two kinds of electrospun nanofibrous meshes with different fiber arrangements (totally non-woven and lattice-like) were fabricated and used for in vitro culture of mesenchymal stem cells (MSCs). By comparing the characteristic marks related to osteogenic differentiation, we found that with prolonged culture time, osteopontin (OPN), osteocalcin (OCN) and alkaline phosphatase (ALP), as well as related genes (Runx2 and Colla genes), were all expressed at higher levels on lattice-like nanofibrous meshes than on non-woven ones. These results indicated that the lattice-like nanofibrous mesh activated the osteogenic differentiation of MSCs owing to changes in cell morphology directed by nanofiber orientations. Compared with pure non-woven nanofibrous meshes, lattice-like ones possessed a combined structure of parallel, magnetic-line-like, and non-woven regions. MSCs adhering onto them had upregulated expression levels of integrin subunits a5 and b1, and activated downstream signaling pathways of Ras homolog gene family member A (RhoA) and extracellular signal-regulated kinase (ERK). When the specific inhibitors PD98059 and Y27632 were used to inhibit phosphorylated ERK and p160 ROCKII activity, respectively, F-actin became disordered and the expression level of Runx2 was downregulated. Thus, we concluded that the scaffold nanotopography may modulate the microenvironment of MSCs and promote their osteogenic differentiation through the RhoA and ERK signaling pathways. These findings provided valuable information on the selection of artificial matrices suitable for MSCs application in bone tissue engineering.

语种: 英语
所属项目编号: 81171726 ; 81101350 ; 2012CB933904 ; 20110001130001
项目资助者: National Natural Science Foundation of China ; Ministry of Science and Technology of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Leading Academic Discipline Project of Beijing Education Bureau
WOS记录号: WOS:000323236300011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66283
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
3.Beijing Univ Chem Technol, State Key Lab Organ Inorgan Composites, Beijing 100029, Peoples R China

Recommended Citation:
Zhu, Jingxian,Cai, Qing,Zhang, Xin,et al. Biological Characteristics of Mesenchymal Stem Cells Grown on Different Topographical Nanofibrous Poly-L-Lactide Meshes[J]. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2013,9(10):1757-1767.
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