IR@PKUHSC  > 北京大学第三临床医学院  > 运动医学研究所
学科主题临床医学
Biological Characteristics of Mesenchymal Stem Cells Grown on Different Topographical Nanofibrous Poly-L-Lactide Meshes
Zhu, Jingxian1; Cai, Qing3; Zhang, Xin1; Hu, Xiaoqing1; Li, La1; Wang, Weiping2; Shao, Zhenxing1; Dai, Linghui1; Cheng, Liyuan3; Yang, Xiaoping3; Zhou, Chunyan2; Ao, Yingfang1
关键词Mesenchymal Stem Cell Extracellular Matrix Nanotopography Osteogenesis Signaling Pathway
刊名JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
2013-10-01
DOI10.1166/jbn.2013.1661
9期:10页:1757-1767
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Nanoscience & Nanotechnology ; Materials Science, Biomaterials
研究领域[WOS]Science & Technology - Other Topics ; Materials Science
关键词[WOS]OSTEOGENIC DIFFERENTIATION ; EXTRACELLULAR-MATRIX ; CYTOSKELETAL TENSION ; TISSUE ; SCAFFOLDS ; ADHESION ; SURFACES ; ENVIRONMENTS ; FIBRONECTIN ; OSTEOBLASTS
英文摘要

The nanotopographical features of artificial scaffolds have complex effects on the biological characteristics of stem cells. They influence cell adhesion, spreading, proliferation, and differentiation; however we have limited knowledge on how these processes occur under nanotopographical cues. In this study, two kinds of electrospun nanofibrous meshes with different fiber arrangements (totally non-woven and lattice-like) were fabricated and used for in vitro culture of mesenchymal stem cells (MSCs). By comparing the characteristic marks related to osteogenic differentiation, we found that with prolonged culture time, osteopontin (OPN), osteocalcin (OCN) and alkaline phosphatase (ALP), as well as related genes (Runx2 and Colla genes), were all expressed at higher levels on lattice-like nanofibrous meshes than on non-woven ones. These results indicated that the lattice-like nanofibrous mesh activated the osteogenic differentiation of MSCs owing to changes in cell morphology directed by nanofiber orientations. Compared with pure non-woven nanofibrous meshes, lattice-like ones possessed a combined structure of parallel, magnetic-line-like, and non-woven regions. MSCs adhering onto them had upregulated expression levels of integrin subunits a5 and b1, and activated downstream signaling pathways of Ras homolog gene family member A (RhoA) and extracellular signal-regulated kinase (ERK). When the specific inhibitors PD98059 and Y27632 were used to inhibit phosphorylated ERK and p160 ROCKII activity, respectively, F-actin became disordered and the expression level of Runx2 was downregulated. Thus, we concluded that the scaffold nanotopography may modulate the microenvironment of MSCs and promote their osteogenic differentiation through the RhoA and ERK signaling pathways. These findings provided valuable information on the selection of artificial matrices suitable for MSCs application in bone tissue engineering.

语种英语
WOS记录号WOS:000323236300011
项目编号81171726 ; 81101350 ; 2012CB933904 ; 20110001130001
资助机构National Natural Science Foundation of China ; Ministry of Science and Technology of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Leading Academic Discipline Project of Beijing Education Bureau
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66283
专题北京大学第三临床医学院_运动医学研究所
北京大学基础医学院
北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
3.Beijing Univ Chem Technol, State Key Lab Organ Inorgan Composites, Beijing 100029, Peoples R China
推荐引用方式
GB/T 7714
Zhu, Jingxian,Cai, Qing,Zhang, Xin,et al. Biological Characteristics of Mesenchymal Stem Cells Grown on Different Topographical Nanofibrous Poly-L-Lactide Meshes[J]. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2013,9(10):1757-1767.
APA Zhu, Jingxian.,Cai, Qing.,Zhang, Xin.,Hu, Xiaoqing.,Li, La.,...&Ao, Yingfang.(2013).Biological Characteristics of Mesenchymal Stem Cells Grown on Different Topographical Nanofibrous Poly-L-Lactide Meshes.JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,9(10),1757-1767.
MLA Zhu, Jingxian,et al."Biological Characteristics of Mesenchymal Stem Cells Grown on Different Topographical Nanofibrous Poly-L-Lactide Meshes".JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 9.10(2013):1757-1767.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhu, Jingxian]的文章
[Cai, Qing]的文章
[Zhang, Xin]的文章
百度学术
百度学术中相似的文章
[Zhu, Jingxian]的文章
[Cai, Qing]的文章
[Zhang, Xin]的文章
必应学术
必应学术中相似的文章
[Zhu, Jingxian]的文章
[Cai, Qing]的文章
[Zhang, Xin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。