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学科主题: 临床医学
题名:
Alteration of heme-oxygenase-carbon monoxide pathway in calcified rat vascular smooth muscle cells
作者: Zhang, BH; Wang, SH; Pang, YZ; Tang, CS; Du, JB
关键词: vascular calcification ; vascular smooth muscle cells ; heme-oxygenase (HO) ; carbon monoxide (CO) ; cyclic guanosine monophosphate (cGMP)
刊名: ZEITSCHRIFT FUR KARDIOLOGIE
发表日期: 2004-02-01
DOI: 10.1007/s00392-004-0989-9
卷: 93, 期:2, 页:109-115
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cardiac & Cardiovascular Systems
研究领域[WOS]: Cardiovascular System & Cardiology
关键词[WOS]: CALCIFICATION ; ATHEROSCLEROSIS ; INHIBITION ; BILIRUBIN ; REGULATOR ; CYCLASE
英文摘要:

Objective The aim of the present study was to investigate the changes in heme-oxygenase (HO)-carbon monoxide (CO)-cyclic guanosine monophosphate (cGMP) pathway in clacified rat vascular smooth muscle cells (VSMCs). Methods Calcification of cultured rat VSMCs was induced by incubation of VSMCs with beta-glycerophosphate. Cellular calcium content, ALP activities and Ca-45 uptake were measured. HO activity, HbCO formation and content of cGMP in VSMCs were determined. Immunocytochemistry for HO-1 expression was observed. Results In comparison of control VSMCs, the cellular calcium content, ALP activity and Ca-45 uptake in calcified VSMCs were obviously increased. Immunocytochemistry showed that HO-1 expression was weak and not well distributed in calcified cells as compared to non-calcified VSMCs, but interestingly, there was stronger staining in calcified nodules than in VSMCs. Compared with VSMCs, HO-1 activity in calcified cells decreased by 42.7% [36.4+/-2.8 pmol (mg Prxh)(-1) vs 63.5x5.3 pmol (mg Prxh)(-1), p<0.01], and HbCO formation decreased by 39.2% (3.38X0.69 mumol/mg Pr vs 5.56+/-0.48 mumol/mg Pr, p<0.05). The cGMP content in calcified VSMCs was 78.1% lower than that of non-calcified VSMCs (4.3+/-0.51 vs 19.6+/-1.2 pmol/mg, p<0.01). Conclusion The results showed that HO-CO-cGMP pathway in calcified vascular cells obviously changed, which might contribute to disturbance of vascular function.

语种: 英语
WOS记录号: WOS:000189307200003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66290
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Peking Univ, Dept Pediat, Hosp 1, Beijing 100034, Peoples R China
2.Peking Univ, Inst Cardiovasc Dis, Hosp 1, Beijing 100034, Peoples R China

Recommended Citation:
Zhang, BH,Wang, SH,Pang, YZ,et al. Alteration of heme-oxygenase-carbon monoxide pathway in calcified rat vascular smooth muscle cells[J]. ZEITSCHRIFT FUR KARDIOLOGIE,2004,93(2):109-115.
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