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Live-cell and super-resolution imaging reveal that the distribution of wall-associated protein A is correlated with the cell chain integrity of Streptococcus mutans
Li, Y.1; Liu, Z.2; Zhang, Y.3; Su, Q. P.2; Xue, B.2; Shao, S.2; Zhu, Y.2; Xu, X.1; Wei, S.1; Sun, Y.2
关键词biofilm cell wall anchored proteins dynamic distribution live cell imaging Streptococcus mutans super-resolution imaging wall-associated protein A
刊名MOLECULAR ORAL MICROBIOLOGY
2015-10-01
DOI10.1111/omi.12100
30期:5页:376-383
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine ; Microbiology
研究领域[WOS]Dentistry, Oral Surgery & Medicine ; Microbiology
关键词[WOS]ARCHITECTURE ; SEQUENCE ; DYNAMICS ; BIOFILMS ; WAPA
英文摘要

Streptococcus mutans is a primary pathogen responsible for dental caries. It has an outstanding ability to form biofilm, which is vital for virulence. Previous studies have shown that knockout of Wall-associated protein A (WapA) affects cell chain and biofilm formation of S.mutans. As a surface protein, the distribution of WapA remains unknown, but it is important to understand the mechanism underlying the function of WapA. This study applied the fluorescence protein mCherry as a reporter gene to characterize the dynamic distribution of WapA in S.mutans via time-lapse and super-resolution fluorescence imaging. The results revealed interesting subcellular distribution patterns of WapA in single, dividing and long chains of S.mutans cells. It appears at the middle of the cell and moves to the poles as the cell grows and divides. In a cell chain, after each round of cell division, such dynamic relocation results in WapA distribution at the previous cell division sites, resulting in a pattern where WapA is located at the boundary of two adjacent cell pairs. This WapA distribution pattern corresponds to the breaking segmentation of wapA deletion cell chains. The dynamic relocation of WapA through the cell cycle increases our understanding of the mechanism of WapA in maintaining cell chain integrity and biofilm formation.

语种英语
WOS记录号WOS:000361007200004
项目编号30973317 ; 31271423 ; 21390412
资助机构Peking University&prime ; s 985 Grant ; National Natural Science Foundation of China
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66291
专题北京大学口腔医学院_口腔颌面外科
北京大学公共卫生学院_毒理学系
作者单位1.Peking Univ, Sch & Hosp Stomatol, Lab Interdisciplinary Studies, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Sch Life Sci, Biodynam Optic Imaging Ctr BIOPIC, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
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GB/T 7714
Li, Y.,Liu, Z.,Zhang, Y.,et al. Live-cell and super-resolution imaging reveal that the distribution of wall-associated protein A is correlated with the cell chain integrity of Streptococcus mutans[J]. MOLECULAR ORAL MICROBIOLOGY,2015,30(5):376-383.
APA Li, Y..,Liu, Z..,Zhang, Y..,Su, Q. P..,Xue, B..,...&Sun, Y..(2015).Live-cell and super-resolution imaging reveal that the distribution of wall-associated protein A is correlated with the cell chain integrity of Streptococcus mutans.MOLECULAR ORAL MICROBIOLOGY,30(5),376-383.
MLA Li, Y.,et al."Live-cell and super-resolution imaging reveal that the distribution of wall-associated protein A is correlated with the cell chain integrity of Streptococcus mutans".MOLECULAR ORAL MICROBIOLOGY 30.5(2015):376-383.
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