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学科主题: 口腔医学
题名:
Live-cell and super-resolution imaging reveal that the distribution of wall-associated protein A is correlated with the cell chain integrity of Streptococcus mutans
作者: Li, Y.1; Liu, Z.2; Zhang, Y.3; Su, Q. P.2; Xue, B.2; Shao, S.2; Zhu, Y.2; Xu, X.1; Wei, S.1; Sun, Y.2
关键词: biofilm ; cell wall anchored proteins ; dynamic distribution ; live cell imaging ; Streptococcus mutans ; super-resolution imaging ; wall-associated protein A
刊名: MOLECULAR ORAL MICROBIOLOGY
发表日期: 2015-10-01
DOI: 10.1111/omi.12100
卷: 30, 期:5, 页:376-383
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine ; Microbiology
研究领域[WOS]: Dentistry, Oral Surgery & Medicine ; Microbiology
关键词[WOS]: ARCHITECTURE ; SEQUENCE ; DYNAMICS ; BIOFILMS ; WAPA
英文摘要:

Streptococcus mutans is a primary pathogen responsible for dental caries. It has an outstanding ability to form biofilm, which is vital for virulence. Previous studies have shown that knockout of Wall-associated protein A (WapA) affects cell chain and biofilm formation of S.mutans. As a surface protein, the distribution of WapA remains unknown, but it is important to understand the mechanism underlying the function of WapA. This study applied the fluorescence protein mCherry as a reporter gene to characterize the dynamic distribution of WapA in S.mutans via time-lapse and super-resolution fluorescence imaging. The results revealed interesting subcellular distribution patterns of WapA in single, dividing and long chains of S.mutans cells. It appears at the middle of the cell and moves to the poles as the cell grows and divides. In a cell chain, after each round of cell division, such dynamic relocation results in WapA distribution at the previous cell division sites, resulting in a pattern where WapA is located at the boundary of two adjacent cell pairs. This WapA distribution pattern corresponds to the breaking segmentation of wapA deletion cell chains. The dynamic relocation of WapA through the cell cycle increases our understanding of the mechanism of WapA in maintaining cell chain integrity and biofilm formation.

语种: 英语
所属项目编号: 30973317 ; 31271423 ; 21390412
项目资助者: Peking University&prime ; s 985 Grant ; National Natural Science Foundation of China
WOS记录号: WOS:000361007200004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66291
Appears in Collections:北京大学口腔医学院_口腔颌面外科_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Lab Interdisciplinary Studies, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Sch Life Sci, Biodynam Optic Imaging Ctr BIOPIC, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China

Recommended Citation:
Li, Y.,Liu, Z.,Zhang, Y.,et al. Live-cell and super-resolution imaging reveal that the distribution of wall-associated protein A is correlated with the cell chain integrity of Streptococcus mutans[J]. MOLECULAR ORAL MICROBIOLOGY,2015,30(5):376-383.
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