北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学深圳医院  > 期刊论文
学科主题: 临床医学
题名:
A resonance light scattering quenching system for studying DNA sequence recognition of actinomycin D
作者: Chen, Zhanguang1; Zhang, Guomin1; Chen, Xi2; Chen, Junhui3; Qian, Sihua1; Li, Qiang1
刊名: ANALYST
发表日期: 2012
DOI: 10.1039/c1an15855d
卷: 137, 期:3, 页:722-728
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: ANTICANCER COMPOUNDS ; SURFACTANTS ; DRUGS ; NANOPARTICLES ; AGGREGATION ; DISTORTIONS ; ASSEMBLIES ; MECHANISM ; PROTEINS ; BINDING
英文摘要:

The DNA sequence recognition study of DNA-targeted anticancer drugs is a theoretical basis for improving the selectivity of anticancer drugs. With the high synergy effect of cocoamidopropyl hydroxy sulfobetaine (HSB), a resonance light scattering (RLS) quenching system for DNA sequence recognition studies of actinomycin D (ACTD) was developed in this contribution. By the strategy, DNA sequence selectivity as well as the recognition mechanisms of ACTD was systematically investigated. The results suggested that ACTD had the selectivity to single-stranded DNA (ssDNA) with an equilibrium constant (K-RLS) of 12.4 mmol mg(-1). Also it had a preference for Guanine and Cytosine bases with a K-RLS of 6.69 L mmol(-1). The selectivity mechanism between ACTD and DNA was also well discussed with the help of UV-Vis absorption spectroscopy. Compared with other methods, the RLS quenching system has the advantages of reliability and speediness, and it avoids complex modification processes and is a better bionic system for the above research. Results obtained from this work would supply a theoretical basis for improving anticancer activity and designing similar anticancer drugs.

语种: 英语
所属项目编号: 39670298 ; YR09009
项目资助者: National Natural Science Foundation of China ; Science Foundation of Shantou University
WOS记录号: WOS:000298969000029
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66296
Appears in Collections:北京大学深圳医院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Shantou Univ, Dept Chem, Shantou 515063, Peoples R China
2.Guangdong Pharmaceut Univ, Guangzhou 510006, Guangdong, Peoples R China
3.Peking Univ, Shenzhen Hosp, Dept Radiol, Shenzhen 518036, Peoples R China

Recommended Citation:
Chen, Zhanguang,Zhang, Guomin,Chen, Xi,et al. A resonance light scattering quenching system for studying DNA sequence recognition of actinomycin D[J]. ANALYST,2012,137(3):722-728.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Chen, Zhanguang]'s Articles
[Zhang, Guomin]'s Articles
[Chen, Xi]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Chen, Zhanguang]‘s Articles
[Zhang, Guomin]‘s Articles
[Chen, Xi]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace