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A resonance light scattering quenching system for studying DNA sequence recognition of actinomycin D
Chen, Zhanguang1; Zhang, Guomin1; Chen, Xi2; Chen, Junhui3; Qian, Sihua1; Li, Qiang1
刊名ANALYST
2012
DOI10.1039/c1an15855d
137期:3页:722-728
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
资助者National Natural Science Foundation of China ; Science Foundation of Shantou University ; National Natural Science Foundation of China ; Science Foundation of Shantou University
研究领域[WOS]Chemistry
关键词[WOS]ANTICANCER COMPOUNDS ; SURFACTANTS ; DRUGS ; NANOPARTICLES ; AGGREGATION ; DISTORTIONS ; ASSEMBLIES ; MECHANISM ; PROTEINS ; BINDING
英文摘要

The DNA sequence recognition study of DNA-targeted anticancer drugs is a theoretical basis for improving the selectivity of anticancer drugs. With the high synergy effect of cocoamidopropyl hydroxy sulfobetaine (HSB), a resonance light scattering (RLS) quenching system for DNA sequence recognition studies of actinomycin D (ACTD) was developed in this contribution. By the strategy, DNA sequence selectivity as well as the recognition mechanisms of ACTD was systematically investigated. The results suggested that ACTD had the selectivity to single-stranded DNA (ssDNA) with an equilibrium constant (K-RLS) of 12.4 mmol mg(-1). Also it had a preference for Guanine and Cytosine bases with a K-RLS of 6.69 L mmol(-1). The selectivity mechanism between ACTD and DNA was also well discussed with the help of UV-Vis absorption spectroscopy. Compared with other methods, the RLS quenching system has the advantages of reliability and speediness, and it avoids complex modification processes and is a better bionic system for the above research. Results obtained from this work would supply a theoretical basis for improving anticancer activity and designing similar anticancer drugs.

语种英语
所属项目编号39670298 ; YR09009
资助者National Natural Science Foundation of China ; Science Foundation of Shantou University ; National Natural Science Foundation of China ; Science Foundation of Shantou University
WOS记录号WOS:000298969000029
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66296
专题北京大学深圳医院
作者单位1.Shantou Univ, Dept Chem, Shantou 515063, Peoples R China
2.Guangdong Pharmaceut Univ, Guangzhou 510006, Guangdong, Peoples R China
3.Peking Univ, Shenzhen Hosp, Dept Radiol, Shenzhen 518036, Peoples R China
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GB/T 7714
Chen, Zhanguang,Zhang, Guomin,Chen, Xi,et al. A resonance light scattering quenching system for studying DNA sequence recognition of actinomycin D[J]. ANALYST,2012,137(3):722-728.
APA Chen, Zhanguang,Zhang, Guomin,Chen, Xi,Chen, Junhui,Qian, Sihua,&Li, Qiang.(2012).A resonance light scattering quenching system for studying DNA sequence recognition of actinomycin D.ANALYST,137(3),722-728.
MLA Chen, Zhanguang,et al."A resonance light scattering quenching system for studying DNA sequence recognition of actinomycin D".ANALYST 137.3(2012):722-728.
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