|Nuclear matrix associated protein PML: an arsenic trioxide apoptosis therapeutic target protein in HepG2 cells|
|Yu, D; Wang, ZH; Zhu, LY; Ec, C|
|关键词||nuclear matrix arsenic trioxide PML protein apoptosis HepG2 cell line|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||ACUTE PROMYELOCYTIC LEUKEMIA ; ESOPHAGEAL-CARCINOMA CELLS ; IN-VITRO ; GROWTH-INHIBITION ; RETINOIC ACID ; CANCER CELLS ; EXPRESSION ; CYTOTOXICITY ; ALPHA ; AS2O3|
Objective To investigate arsenic trioxide (As2O3)-induced apoptosis and the effects on cell nuclear matrix related protein promyelocytic leukaemia (PML).
Methods HepG2 cells were cultured in MEM medium and treated with 0.5, 2, 5 and 10 mumol/L As2O3 for either 24 h or 96 h at each concentration. In situ terminal deoxynucleotidyl transferase (TdT) labeling (TUNEL) and DNA ladders were used to detect apoptosis. Confocal microscopy and Western blotting were used to observe the expression of PML.
Results The growth rates of HepG2 cells were slower in the As2O3 treated than the untreated control group. DNA ladder and TUNEL positive apoptotic cells could be detected in As2O3 treated groups. The expression of PML decreased in HepG2 cells with 2 mumol/L As2O3 treatment. Confocal images demonstrated that the expression, of PML protein in HepG2 cell nuclei decreased after treatment with 2 mumol/L As2O3, and micropunctates characteristic of PML protein in HepG2 cell nuclei disappeared after treatment with 5 mumol/L As2O3.
Conclusions Our results show that arsenic trioxide can significantly inhibit the growth of HepG2 cells in vitro. As2O3 induces apoptosis in HepG2 tumor cells in a time and concentration dependent manner. As2O3 may degrade the PML protein in HepG2 cell nuclei. The decreased expression of PML in As2O3 treated tumor cells is most likely to be caused by apoptosis. Nuclear matrix associated protein PML could be the target of As2O3 therapy.
|作者单位||1.Beijing Univ, Shenzhen Hosp, Dept Pathol, Shenzhen 518036, Peoples R China|
2.Beijing Univ, Shenzhen Hosp, Dept Surg, Shenzhen 518036, Peoples R China
3.Chinese Univ Hong Kong, Dept Anat, Hong Kong, Hong Kong, Peoples R China
|Yu, D,Wang, ZH,Zhu, LY,et al. Nuclear matrix associated protein PML: an arsenic trioxide apoptosis therapeutic target protein in HepG2 cells[J]. CHINESE MEDICAL JOURNAL,2003,116(1):93-98.|
|APA||Yu, D,Wang, ZH,Zhu, LY,&Ec, C.(2003).Nuclear matrix associated protein PML: an arsenic trioxide apoptosis therapeutic target protein in HepG2 cells.CHINESE MEDICAL JOURNAL,116(1),93-98.|
|MLA||Yu, D,et al."Nuclear matrix associated protein PML: an arsenic trioxide apoptosis therapeutic target protein in HepG2 cells".CHINESE MEDICAL JOURNAL 116.1(2003):93-98.|