北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科  > 期刊论文
学科主题: 临床医学
题名:
Molecular typing study of the Microsporum canis strains isolated from an outbreak of tinea capitis in a school
作者: Yu, J; Wan, Z; Chen, W; Wang, WL; Li, RY
关键词: tinea capits ; outbreak ; M. canis ; RAPD ; NTS ; DNA sequencing
刊名: MYCOPATHOLOGIA
发表日期: 2004
卷: 157, 期:1, 页:37-41
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Mycology
研究领域[WOS]: Mycology
关键词[WOS]: INTERGENIC SPACER SEQUENCES ; PCR AMPLIFICATION ; IDENTIFICATION ; UNIT
英文摘要:

Tinea capitis is a dermatophyte infection of the scalp that occurs most often in prepubescent children. Tinea capitis may be transmitted by shared use of contaminated hairbrush, by contact with fomites or by direct physical contact with an infected person. Occasionally, outbreak of tinea capitis would happen under some special conditions. Last year, we found an outbreak of tinea capitis in a school due to Microsporum canis. In epidemiological study, we performed the prevalence survey to all of the exposed persons by physical examinations and mycological laboratory tests, including KOH preparation and fungal cultures. We also investigated the environment in the school. In molecular typing study of the M. canis isolated from patients and the environment, random primer amplification polymorphic DNA (RAPD) method, the specific amplification of subrepeat element in the ribosomal DNA nontranscribed spacer (NTS), and the analysis of DNA sequence in the intertranscribed spacer (ITS) of rDNA were performed. The total number of exposed children was seventy-one, among them forty-two were attacked by tinea capitis. The ratio between boy and girl was 13:1. The ages of the patients was ranged from 3.5 years old to 10 years old. Four patients bred cat or dog as pet. Most patients appeared noninflammatory type of tinea capitis and several patients were inflammatory type. Under microscopic examination the invaded hair were all ectothrix. The pathogens isolated from these patients were M. canis. And we also isolated M. canis from the carpet and the pillowcase in the school. The patterns of total strains of M. canis in the RAPD method and PCR amplification of the rDNA NTS region study were identical, and the isolates from patients and the environment contained the same DNA sequences in the ITS region. The outbreak of tinea capitis was caused by M. canis. The M. canis isolated from patients and from the environment were probably the same origin.

语种: 英语
WOS记录号: WOS:000188120500004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66302
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Hosp 1, Dept Dermatol, Res Ctr Med Mycol, Beijing 100034, Peoples R China

Recommended Citation:
Yu, J,Wan, Z,Chen, W,et al. Molecular typing study of the Microsporum canis strains isolated from an outbreak of tinea capitis in a school[J]. MYCOPATHOLOGIA,2004,157(1):37-41.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Yu, J]'s Articles
[Wan, Z]'s Articles
[Chen, W]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Yu, J]‘s Articles
[Wan, Z]‘s Articles
[Chen, W]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace