IR@PKUHSC  > 北京大学第一临床医学院  > 皮肤性病科
学科主题临床医学
Molecular typing study of the Microsporum canis strains isolated from an outbreak of tinea capitis in a school
Yu, J; Wan, Z; Chen, W; Wang, WL; Li, RY
关键词tinea capits outbreak M. canis RAPD NTS DNA sequencing
刊名MYCOPATHOLOGIA
2004
157期:1页:37-41
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Mycology
研究领域[WOS]Mycology
关键词[WOS]INTERGENIC SPACER SEQUENCES ; PCR AMPLIFICATION ; IDENTIFICATION ; UNIT
英文摘要

Tinea capitis is a dermatophyte infection of the scalp that occurs most often in prepubescent children. Tinea capitis may be transmitted by shared use of contaminated hairbrush, by contact with fomites or by direct physical contact with an infected person. Occasionally, outbreak of tinea capitis would happen under some special conditions. Last year, we found an outbreak of tinea capitis in a school due to Microsporum canis. In epidemiological study, we performed the prevalence survey to all of the exposed persons by physical examinations and mycological laboratory tests, including KOH preparation and fungal cultures. We also investigated the environment in the school. In molecular typing study of the M. canis isolated from patients and the environment, random primer amplification polymorphic DNA (RAPD) method, the specific amplification of subrepeat element in the ribosomal DNA nontranscribed spacer (NTS), and the analysis of DNA sequence in the intertranscribed spacer (ITS) of rDNA were performed. The total number of exposed children was seventy-one, among them forty-two were attacked by tinea capitis. The ratio between boy and girl was 13:1. The ages of the patients was ranged from 3.5 years old to 10 years old. Four patients bred cat or dog as pet. Most patients appeared noninflammatory type of tinea capitis and several patients were inflammatory type. Under microscopic examination the invaded hair were all ectothrix. The pathogens isolated from these patients were M. canis. And we also isolated M. canis from the carpet and the pillowcase in the school. The patterns of total strains of M. canis in the RAPD method and PCR amplification of the rDNA NTS region study were identical, and the isolates from patients and the environment contained the same DNA sequences in the ITS region. The outbreak of tinea capitis was caused by M. canis. The M. canis isolated from patients and from the environment were probably the same origin.

语种英语
WOS记录号WOS:000188120500004
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66302
Collection北京大学第一临床医学院_皮肤性病科
作者单位Peking Univ, Hosp 1, Dept Dermatol, Res Ctr Med Mycol, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Yu, J,Wan, Z,Chen, W,et al. Molecular typing study of the Microsporum canis strains isolated from an outbreak of tinea capitis in a school[J]. MYCOPATHOLOGIA,2004,157(1):37-41.
APA Yu, J,Wan, Z,Chen, W,Wang, WL,&Li, RY.(2004).Molecular typing study of the Microsporum canis strains isolated from an outbreak of tinea capitis in a school.MYCOPATHOLOGIA,157(1),37-41.
MLA Yu, J,et al."Molecular typing study of the Microsporum canis strains isolated from an outbreak of tinea capitis in a school".MYCOPATHOLOGIA 157.1(2004):37-41.
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