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学科主题: 基础医学
题名:
Alpha-fetoprotein: a new member of intracellular signal molecules in regulation of the PI3K/AKT signaling in human hepatoma cell lines
作者: Li, Mengsen1,2; Li, Hui1; Li, Chaoying1; Wang, Shanshan1; Jiang, Wei1; Liu, Zhongmin1; Zhou, Sheng2; Liu, Xinhua1; McNutt, Michael A.3; Li, Gang1
关键词: AFP ; signal molecule ; PTEN ; PI3K ; AKT ; hepatoma
刊名: INTERNATIONAL JOURNAL OF CANCER
发表日期: 2011-02-01
DOI: 10.1002/ijc.25373
卷: 128, 期:3, 页:524-532
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: MU-OPIOID RECEPTOR ; HEPATOCELLULAR-CARCINOMA ; PEPTIDES ; PATHWAY ; CANCER ; LYMPHOCYTES ; APOPTOSIS ; GROWTH ; PTEN ; METASTASIS
英文摘要:

Despite its well-defined role as a serum growth factor during fetal liver development and hepatic oncogenesis, the biological significance of cytoplasmic alpha-fetoprotein (AFP) remains incompletely understood. Here, we provide evidence to illustrate that cytoplasmic AFP may function as a regulator in the phosphatidylinositol 3-kinase (PI3K)/AKT pathway in human hepatocellular carcinoma cells. The results demonstrated colocalization and interaction of AFP and phosphatase and tensin homolog deleted on chromosome 10 (PTEN) in the cytoplasm of AFP-producing Bel 7402 and HepG2 cells, with an interaction distance of 12.6 +/- 2.7 angstrom as determined with the fluorescence resonance energy transfer technique. Knockdown of AFP mRNA or inhibition of AFP expression by all trans-retinoic acid resulted in enhancement of the PTEN level with a synchronous decrease in phosphorylated AKT. Transfection of the afp gene into HLE cells (originally AFP negative) led to a significant activation of AKT signaling. The inhibition of PI3K signaling by LY 294002 was simultaneously reversed by transfection, accompanied by diminution of all trans-retinoic acid-induced upregulation of PTEN and enhancement of cell growth. In conclusion, these results demonstrate that cytoplasmic AFP is involved in regulation of hepatocellular growth and tumorigenesis.

语种: 英语
所属项目编号: 30671856 ; 30772536 ; 30660071 ; 30760090 ; 7101006 ; 309034
项目资助者: National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Natural Science Foundation of Hainan Province
WOS记录号: WOS:000285264000003
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66310
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Peking Univ Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
2.Hainan Med Coll, Key Lab Mol Biol, Haikou, Hainan, Peoples R China
3.Peking Univ Hlth Sci Ctr, Dept Pathol, Beijing 100191, Peoples R China

Recommended Citation:
Li, Mengsen,Li, Hui,Li, Chaoying,et al. Alpha-fetoprotein: a new member of intracellular signal molecules in regulation of the PI3K/AKT signaling in human hepatoma cell lines[J]. INTERNATIONAL JOURNAL OF CANCER,2011,128(3):524-532.
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