|Effect of curettage and copper wire on rabbit endometrium: a novel rabbit model of endometrial mechanical injury|
|Li Li1; Shi Jing2; Zhang Qiu-fang1; Yan Jie1; Yan Li-ying1; Shen Fei2; Qiao Jie1; Feng Huai-liang1,3|
|关键词||endometrium injury repair animal model|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||VAGINAL SILDENAFIL ; WOUND REPAIR ; IN-VITRO ; REGENERATION ; THERAPY ; CITRATE ; PHASE ; WOMEN ; IVF|
Background It remains almost a helpless situation for the recurrent implantation failure and pregnancy loss caused by endometrial injury at present. The purpose of this study was to develop a rabbit model of endometrial mechanical injury that could provide a research platform for this difficult clinical predicament.
Methods Three experiments were conducted. Experiment 1: Curettages in both uterus horns and copper wire inserting after curettage (double-injury) in one horn. The histological changes were monitored at 0, 24, 48, 72 hours, as well as in 1 and 2 weeks after operation. Experiment 2: Direct copper wire inserting in one horn and double-injury in other horn. The wires in both horns were removed after 2 weeks. The histological changes were recorded at 0, 1 and 2 weeks after wire removal. Experiment 3: Double-injury procedure in one horn was performed and wire was removed after 2 weeks; another horn was remained normal to serve as control. Histological changes were recorded, tissue areas were measured, and proliferation indices (PIs, %) were calculated at 1, 2, 4 and 8 weeks after wire removal, respectively.
Results The experiments revealed that the injured endometrium by simple curettage or copper wire could be fully repaired. While the endometrial regeneration was severely impaired by double-injury, both areas of endometrium and uterine cavity decreased (P <0.05); both Pis of glandular epithelial and stromal cells increased and reached maximum at 4 weeks (P <0.05), but returned by 8 weeks.
Conclusion This study demonstrated that a rabbit model of endometrial injury could be effectively established through a double-injury procedure of curettage and copper wire with comparable clinical index. Chin Med J 2011;124(11):1708-1713
|项目编号||2007-1002 ; BMU20090514-603 ; JSW08-7-08|
|资助机构||Capital Medical Development Scientific Research Fund ; Fundamental Research Funds for the Central Universities ; National Population and Family Planning Commission of China|
|作者单位||1.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China|
2.Peking Univ, Hosp 3, Ctr Lab Anim Sci, Beijing 100191, Peoples R China
3.Hofstra Univ, Sch Med, N Shore Univ Hosp LIJ, Ctr Human Reprod,Dept Obstet & Gynecol, Manhasset, NY 10030 USA
|Li Li,Shi Jing,Zhang Qiu-fang,et al. Effect of curettage and copper wire on rabbit endometrium: a novel rabbit model of endometrial mechanical injury[J]. CHINESE MEDICAL JOURNAL,2011,124(11):1708-1713.|
|APA||Li Li.,Shi Jing.,Zhang Qiu-fang.,Yan Jie.,Yan Li-ying.,...&Feng Huai-liang.(2011).Effect of curettage and copper wire on rabbit endometrium: a novel rabbit model of endometrial mechanical injury.CHINESE MEDICAL JOURNAL,124(11),1708-1713.|
|MLA||Li Li,et al."Effect of curettage and copper wire on rabbit endometrium: a novel rabbit model of endometrial mechanical injury".CHINESE MEDICAL JOURNAL 124.11(2011):1708-1713.|