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学科主题: 临床医学
题名:
Comparative analysis of the protein profiles from primary gastric tumors and their adjacent regions: MAWBP could be a new protein candidate involved in gastric cancer
作者: Zhang, Jun; Kang, Bin; Tan, Xiaohui; Bai, Zhigang; Liang, Yumei; Xing, Rui; Shao, Jianmin; Xu, Ningzhi; Wang, Rong; Liu, Siqi; Lu, Youyong
关键词: gastric cancer ; MAWBP ; MAWD ; proteomics ; tissue microarray
刊名: JOURNAL OF PROTEOME RESEARCH
发表日期: 2007-11-01
DOI: 10.1021/pr0703425
卷: 6, 期:11, 页:4423-4432
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: PROTEOMIC APPROACH ; PROGNOSTIC-FACTORS ; BREAST-CANCER ; BIOMARKERS ; IDENTIFICATION ; CARCINOMA ; TISSUES ; GENE ; ELECTROPHORESIS ; PATTERNS
英文摘要:

The study of tumor biomarkers is generally facilitated by the adoption of proteomic strategies. With limitations of techniques and individual varieties of biological samples, the biomarkers for gastric cancer (GC) have not reached a common agreement derived from the proteomic investigations. Herein, we reported a new set of data for screening the biomarkers from the gastric tissues, on the basis of the proteomic strategy developed in our laboratory. Ten pairs of the clinic samples were collected and treated with protein extraction. The gastric, proteins were well-resolved by 2-DE, and the GC-associated proteins were identified by MALDI-TOF/TOF MS following image analysis, including 12 up-regulated and 13 down-regulated unique ones. MAWBP was found to be one of the new GC proteins which appeared with lower expression in the GC tissues. We expanded a systematical examination to deeply pursue the relevance between MAWBP and GC. Quantitatively, we measured the expression of MAWBP with Western blot and Real-Time PCR. Extendedly, we estimated the existence of MAWBP with. immunohistochemical staining in a large number of the GC cases. Specifically, we inquired whether MAWD, a protein with high affinity to MAWBP, could coexpress and interact with MAWBP in vivo. On the basis of all the results, we concluded that MAWBP could be a new GC-related protein even though its physiological roles remain unexplored.

语种: 英语
WOS记录号: WOS:000250718700034
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66318
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Beijing 100034, Peoples R China
2.Beijing Proteom Inst, Beijing 101318, Peoples R China
3.Chinese Acad Sci, Beijing Genom Inst, Beijing 101318, Peoples R China
4.Peking Univ, Beijing Canc Hosp Inst, Sch Oncol, Beijing 100036, Peoples R China
5.CUNY Mt Sinai Sch Med, Dept Genet & Genom Sci, New York, NY 10029 USA
6.PLA, Dept Pathol, Gen Hosp, Beijing 100853, Peoples R China

Recommended Citation:
Zhang, Jun,Kang, Bin,Tan, Xiaohui,et al. Comparative analysis of the protein profiles from primary gastric tumors and their adjacent regions: MAWBP could be a new protein candidate involved in gastric cancer[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(11):4423-4432.
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