IR@PKUHSC  > 北京大学药学院
学科主题药学
Interactions of KChIP4a and its mutants with Ca2+ or Kv4.3 N-terminus by affinity capillary electrophoresis
Li, Meina1,2; Lei, Lei1,3; Jia, Linghan1,3; Ling, Xiaomei1,2; Zhang, Jianmei1,2; Zhao, Yiran1,2; Wang, KeWei1,3
关键词ACE Interaction Ca2+ KChIPs KvN
刊名ANALYTICAL BIOCHEMISTRY
2014-03-15
DOI10.1016/j.ab.2013.12.015
449页:99-105
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical
资助者National Natural Science Foundation of China ; Open Foundation of State Key Laboratory of Natural and Biomimetic Drugs ; Specialized Research Fund for the Doctoral Program of Higher Education of China ; Ministry of Science and Technology of China ; National Science Foundation of China ; National Natural Science Foundation of China ; Open Foundation of State Key Laboratory of Natural and Biomimetic Drugs ; Specialized Research Fund for the Doctoral Program of Higher Education of China ; Ministry of Science and Technology of China ; National Science Foundation of China
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]POTASSIUM CHANNELS ; BINDING CONSTANTS ; K+ CHANNELS ; MODULATION ; PROTEINS ; SUBUNITS ; DOMAINS
英文摘要

The specific binding of auxiliary Kv channel-interacting proteins (KChIPs) to the N terminus of Kv4 poreforming alpha-subunits results in modulation of gating properties, surface expression, and subunit assembly of Kv4 channels. However, the interactions between KChIPs and Kv4 remain elusive. Thus, affinity capillary electrophoresis (ACE) was employed to quantitatively evaluate the interactions between KChIPs and Kv4.3 N terminus (KvN) and between KChIP4a/related mutants and Ca2+ for the first time. The mobility ratio, derivatives calculated from the mobility shift method, was used to deduce the binding constants (K-b). As a result, the binding constants for KChIP4a/KvN and KChIP1/KvN complexes were (8.32 +/- 1.66) x 10(6) L mol(-1) and (5.26 +/- 0.71) x 10(6) L mol(-1), respectively. In addition, in the presence of calcium (10 mu mol L-1), the binding constant of KChIP4a/KvN increased to (6.72 +/- 1.66) x 10(7) L mol(-1). In addition, the binding constant of KChIP4a with Ca2+ was (7.1 +/- 1.5) x 10(7) L mol(-1). Besides, studies on the effect of truncated mutants revealed that the third EF hand of KChIP4a was related to high-affinity binding with Ca2+, and the integrity of the molecular structure of KChIP4a was important for Ca2+ binding. This method profits from small samples, rapid analysis, and simple operation without being time-consuming. (C) 2013 Elsevier Inc. All rights reserved.

语种英语
所属项目编号30872292 ; 90813025 ; 81072612 ; 81373372 ; SKL2012004 ; 20110001110021 ; 2013CB531300 ; 81221002
资助者National Natural Science Foundation of China ; Open Foundation of State Key Laboratory of Natural and Biomimetic Drugs ; Specialized Research Fund for the Doctoral Program of Higher Education of China ; Ministry of Science and Technology of China ; National Science Foundation of China ; National Natural Science Foundation of China ; Open Foundation of State Key Laboratory of Natural and Biomimetic Drugs ; Specialized Research Fund for the Doctoral Program of Higher Education of China ; Ministry of Science and Technology of China ; National Science Foundation of China
WOS记录号WOS:000332816100014
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66321
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut Anal, Beijing 100191, Peoples R China
3.Peking Univ, Dept Mol & Cellular Pharmacol, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Li, Meina,Lei, Lei,Jia, Linghan,et al. Interactions of KChIP4a and its mutants with Ca2+ or Kv4.3 N-terminus by affinity capillary electrophoresis[J]. ANALYTICAL BIOCHEMISTRY,2014,449:99-105.
APA Li, Meina.,Lei, Lei.,Jia, Linghan.,Ling, Xiaomei.,Zhang, Jianmei.,...&Wang, KeWei.(2014).Interactions of KChIP4a and its mutants with Ca2+ or Kv4.3 N-terminus by affinity capillary electrophoresis.ANALYTICAL BIOCHEMISTRY,449,99-105.
MLA Li, Meina,et al."Interactions of KChIP4a and its mutants with Ca2+ or Kv4.3 N-terminus by affinity capillary electrophoresis".ANALYTICAL BIOCHEMISTRY 449(2014):99-105.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Meina]的文章
[Lei, Lei]的文章
[Jia, Linghan]的文章
百度学术
百度学术中相似的文章
[Li, Meina]的文章
[Lei, Lei]的文章
[Jia, Linghan]的文章
必应学术
必应学术中相似的文章
[Li, Meina]的文章
[Lei, Lei]的文章
[Jia, Linghan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。