IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Study on the adoption of Schwann cell phenotype by bone marrow stromal cells in vitro and in vivo
Zhao, FQ; Zhang, PX; He, XJ; Du, C; Fu, ZG; Zhang, DY; Jiang, BG
关键词bone marrow stromal cells differentiation Schwann cells
刊名BIOMEDICAL AND ENVIRONMENTAL SCIENCES
2005-10-01
18期:5页:326-333
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Environmental Sciences ; Public, Environmental & Occupational Health
研究领域[WOS]Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
关键词[WOS]NERVE REGENERATION ; STEM-CELLS ; DIFFERENTIATE ; TRANSPLANTATION ; ENGRAFTMENT ; BRAINS ; FUSION ; RATS
英文摘要

Objective: To explore the possibilities of bone marrow stromal cells (MSCs) to adopt Schwann cell phenotype in vitro and in vivo in SD rats. Methods: MSCs were obtained from tibia and femur bone marrow and cultured in culture flasks. Beta-mercaptoethanol followed by retinoic acid, forskolin, basic-FGF, PDGF and heregulin were added to induce differentiation of MSCs′. Schwann cell markers, p75, S-100 and GFAP were used to discriminate induced properties of MSCs′ by immunofluorescent staining. PKH-67-labelled MSCs were transplanted into the mechanically injured rat sciatic nerve, and laser confocal microscopy was performed to localize the PKH67 labelled MSCs in the injured sciatic nerve two weeks after the operation. Fluorescence PKH67 attenuation rule was evaluated by flow cytometry in vitro. Results: MSCs changed morphologically into cells resembling primary cultured Schwann cells after their induction in vitro. In vivo, a large number of MSCs were cumulated within the layer of epineurium around the injured nerve and expressed Schwann cell markers, p75, S-100,and GFAP. Conclusion: MSCs are able to support nerve fiber regeneration and re-myelination by taking on Schwann cell function, and can be potentially used as possible substitutable cells for artificial nerve conduits to promote nerve regeneration.

语种英语
WOS记录号WOS:000232650200008
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66330
专题北京大学第二临床医学院
作者单位Peking Univ, Dept Orthoped & Trauma, Peoples Hosp, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Zhao, FQ,Zhang, PX,He, XJ,et al. Study on the adoption of Schwann cell phenotype by bone marrow stromal cells in vitro and in vivo[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2005,18(5):326-333.
APA Zhao, FQ.,Zhang, PX.,He, XJ.,Du, C.,Fu, ZG.,...&Jiang, BG.(2005).Study on the adoption of Schwann cell phenotype by bone marrow stromal cells in vitro and in vivo.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,18(5),326-333.
MLA Zhao, FQ,et al."Study on the adoption of Schwann cell phenotype by bone marrow stromal cells in vitro and in vivo".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 18.5(2005):326-333.
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