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学科主题临床医学
Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells
Jing, Hongmei1,2; Wang, Jing1,2; Yang, Ping1,2; Ke, Xiaoyan1,2; Xia, Guohua3; Chen, Baoan3
关键词magnetic nanoparticles Raji cells apoptosis p53 NF-kappa B
刊名INTERNATIONAL JOURNAL OF NANOMEDICINE
2010
DOI10.2147/IJN.S14957
5页:999-1004
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
研究领域[WOS]Science & Technology - Other Topics ; Pharmacology & Pharmacy
关键词[WOS]NF-KAPPA-B ; CANCER ; THERAPY
英文摘要

The purpose of this study was to investigate the potential effects of combination therapy using magnetic nanoparticles of Fe3O4 (MNP-Fe3O4) and chemotherapeutic drugs on lymphoma cells. Proliferation, inhibition, and viability of Raji cells were detected by MTT and trypan blue exclusion. The percentage of cells undergoing apoptosis was detected by flow cytometry using fluorescein isothiocyanate-annexin V and propidium iodide staining. p53 and nuclear factor-kappa B (NF-kappa B) protein levels were measured by Western blot. The results showed that proliferation of Raji cells was inhibited by adriamycin or daunorubicin in a dose-and time-dependent manner. Cell sensitivity was improved and the 50% inhibitory concentrations of adriamycin and daunorubicin decreased when combined with a MNP-Fe3O4 carrier. Interestingly, increased apoptosis in Raji lymphoma cells was accompanied by upregulation of p53 protein and downregulation of NF-kappa B protein. Furthermore, the combination of MNP-Fe3O4 with adriamycin or daunorubicin increased p53 protein levels and decreased NF-kappa B protein levels more than adriamycin or daunorubicin alone, indicating that MNP-Fe3O4 could enhance the effect of chemotherapeutic drugs on p53 and NF-kappa B. Similar results for cell apoptosis and protein expression were not observed for the groups treated with dexamethasone +/- MNP-Fe3O4 (P > 0.05). These findings suggest a potential clinical application for MNP-Fe3O4 in combination with daunorubicin or adriamycin in the treatment of lymphoma.

语种英语
WOS记录号WOS:000284397200002
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66331
专题北京大学第三临床医学院_血液内科
北京大学护理学院_护理学院
作者单位1.Peking Univ, Hosp 3, Dept Hematol, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Lymphoma Res Ctr, Beijing 100191, Peoples R China
3.Southeast Univ, Sch Clin Med, Affiliated Zhongda Hosp, Dept Hematol, Nanjing, Peoples R China
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GB/T 7714
Jing, Hongmei,Wang, Jing,Yang, Ping,et al. Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2010,5:999-1004.
APA Jing, Hongmei,Wang, Jing,Yang, Ping,Ke, Xiaoyan,Xia, Guohua,&Chen, Baoan.(2010).Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells.INTERNATIONAL JOURNAL OF NANOMEDICINE,5,999-1004.
MLA Jing, Hongmei,et al."Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells".INTERNATIONAL JOURNAL OF NANOMEDICINE 5(2010):999-1004.
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