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学科主题: 临床医学
题名:
Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells
作者: Jing, Hongmei1,2; Wang, Jing1,2; Yang, Ping1,2; Ke, Xiaoyan1,2; Xia, Guohua3; Chen, Baoan3
关键词: magnetic nanoparticles ; Raji cells ; apoptosis ; p53 ; NF-kappa B
刊名: INTERNATIONAL JOURNAL OF NANOMEDICINE
发表日期: 2010
DOI: 10.2147/IJN.S14957
卷: 5, 页:999-1004
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Nanoscience & Nanotechnology ; Pharmacology & Pharmacy
研究领域[WOS]: Science & Technology - Other Topics ; Pharmacology & Pharmacy
关键词[WOS]: NF-KAPPA-B ; CANCER ; THERAPY
英文摘要:

The purpose of this study was to investigate the potential effects of combination therapy using magnetic nanoparticles of Fe3O4 (MNP-Fe3O4) and chemotherapeutic drugs on lymphoma cells. Proliferation, inhibition, and viability of Raji cells were detected by MTT and trypan blue exclusion. The percentage of cells undergoing apoptosis was detected by flow cytometry using fluorescein isothiocyanate-annexin V and propidium iodide staining. p53 and nuclear factor-kappa B (NF-kappa B) protein levels were measured by Western blot. The results showed that proliferation of Raji cells was inhibited by adriamycin or daunorubicin in a dose-and time-dependent manner. Cell sensitivity was improved and the 50% inhibitory concentrations of adriamycin and daunorubicin decreased when combined with a MNP-Fe3O4 carrier. Interestingly, increased apoptosis in Raji lymphoma cells was accompanied by upregulation of p53 protein and downregulation of NF-kappa B protein. Furthermore, the combination of MNP-Fe3O4 with adriamycin or daunorubicin increased p53 protein levels and decreased NF-kappa B protein levels more than adriamycin or daunorubicin alone, indicating that MNP-Fe3O4 could enhance the effect of chemotherapeutic drugs on p53 and NF-kappa B. Similar results for cell apoptosis and protein expression were not observed for the groups treated with dexamethasone +/- MNP-Fe3O4 (P > 0.05). These findings suggest a potential clinical application for MNP-Fe3O4 in combination with daunorubicin or adriamycin in the treatment of lymphoma.

语种: 英语
WOS记录号: WOS:000284397200002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66331
Appears in Collections:北京大学第三临床医学院_血液内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Hematol, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Lymphoma Res Ctr, Beijing 100191, Peoples R China
3.Southeast Univ, Sch Clin Med, Affiliated Zhongda Hosp, Dept Hematol, Nanjing, Peoples R China

Recommended Citation:
Jing, Hongmei,Wang, Jing,Yang, Ping,et al. Magnetic Fe3O4 nanoparticles and chemotherapy agents interact synergistically to induce apoptosis in lymphoma cells[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2010,5:999-1004.
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