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学科主题: 临床医学
题名:
The molecular impact of pigment epithelium-derived factor, PEDF, on lung cancer cells and the clinical significance
作者: Chen, Jinfeng1,2; Ye, Lin1; Zhang, Lijian2; Jiang, Wen G.1
关键词: PEDF ; lung cancer ; cellular motility ; metastasis ; prognosis
刊名: INTERNATIONAL JOURNAL OF ONCOLOGY
发表日期: 2009-07-01
DOI: 10.3892/ijo_00000324
卷: 35, 期:1, 页:159-166
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: HUMAN BREAST-CANCER ; FACTOR EXPRESSION ; IN-VIVO ; KAPPA-B ; GROWTH ; ANGIOGENESIS ; GENES ; TUMOR ; OVEREXPRESSION ; PROGRESSION
英文摘要:

Pigment epithelium-derived factor (PEDF) is an endogenous protein factor that has been shown to act as antiangiognesis factor. The present study aimed to determine the direct biological effects of PEDF on lung cancer cells and deduce a clinical relevance in patients with lung cancer, major cause of death worldwide in which the knowledge of PEDF remains poor. We constructed a mammalian expression system for human PEDF produced recombinant PEDF (rhPEDF) protein from 3T3 cells. The expression of PEDF was examined using SDS-PAGE and Western blot analysis. Using the rhPEDF protein, we investigated the biological function of PEDF in the lung cancer cells as well as endothelial cells. PEDF expression levels were assessed in a cohort of human lung cancer specimen (77 pairs of matched normal and tumour tissues), in association with patient clinical variables and Survival, using quantitative analysis of PEDF. In vitro, we found that administration of rhPEDF on two lung cancer cell lines (A549 and SK-MES1) significantly reduced tumour cell growth (P<0.05) with no significant effect on the growth of vascular cell line (HECV). We also found that rhPEDF significantly decreased lung cancer motility and adhesion to extracellular matrix (Matrigel) when compared with the control cells (P<0.05). We showed that reduced PEDF levels in lung cancer tissues significantly correlated with lymph node metastasis and an overall poor prognosis in the lung cancer patients. PEDF Suppresses the growth and Motility Of lung cancer cells and has a significant correlation with the clinical outcome of the patients. These results contribute to our understanding of the molecular mechanisms of PEDF and indicates a potential prognostic and therapeutic impact of PEDF in lung cancer.

语种: 英语
项目资助者: Cancer Research Wales and Cancer Research, UK ; Beijing Municipal Science Technology ; Albert Hung Foundation (HK)
WOS记录号: WOS:000267231400018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66347
Appears in Collections:北京大学临床肿瘤学院_胸外科_期刊论文

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作者单位: 1.Cardiff Univ, Metastasis & Angiogenesis Res Grp, Dept Surg, Sch Med, Cardiff CF14 4XN, S Glam, Wales
2.Peking Univ, Key Lab Carcinogenesis & Translat Res, Dept Thorac Surg, Beijing Canc Hosp & Inst,Sch Oncol,Minist Educ, Beijing 100036, Peoples R China

Recommended Citation:
Chen, Jinfeng,Ye, Lin,Zhang, Lijian,et al. The molecular impact of pigment epithelium-derived factor, PEDF, on lung cancer cells and the clinical significance[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2009,35(1):159-166.
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