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学科主题临床医学
The molecular impact of pigment epithelium-derived factor, PEDF, on lung cancer cells and the clinical significance
Chen, Jinfeng1,2; Ye, Lin1; Zhang, Lijian2; Jiang, Wen G.1
关键词PEDF lung cancer cellular motility metastasis prognosis
刊名INTERNATIONAL JOURNAL OF ONCOLOGY
2009-07-01
DOI10.3892/ijo_00000324
35期:1页:159-166
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]HUMAN BREAST-CANCER ; FACTOR EXPRESSION ; IN-VIVO ; KAPPA-B ; GROWTH ; ANGIOGENESIS ; GENES ; TUMOR ; OVEREXPRESSION ; PROGRESSION
英文摘要

Pigment epithelium-derived factor (PEDF) is an endogenous protein factor that has been shown to act as antiangiognesis factor. The present study aimed to determine the direct biological effects of PEDF on lung cancer cells and deduce a clinical relevance in patients with lung cancer, major cause of death worldwide in which the knowledge of PEDF remains poor. We constructed a mammalian expression system for human PEDF produced recombinant PEDF (rhPEDF) protein from 3T3 cells. The expression of PEDF was examined using SDS-PAGE and Western blot analysis. Using the rhPEDF protein, we investigated the biological function of PEDF in the lung cancer cells as well as endothelial cells. PEDF expression levels were assessed in a cohort of human lung cancer specimen (77 pairs of matched normal and tumour tissues), in association with patient clinical variables and Survival, using quantitative analysis of PEDF. In vitro, we found that administration of rhPEDF on two lung cancer cell lines (A549 and SK-MES1) significantly reduced tumour cell growth (P<0.05) with no significant effect on the growth of vascular cell line (HECV). We also found that rhPEDF significantly decreased lung cancer motility and adhesion to extracellular matrix (Matrigel) when compared with the control cells (P<0.05). We showed that reduced PEDF levels in lung cancer tissues significantly correlated with lymph node metastasis and an overall poor prognosis in the lung cancer patients. PEDF Suppresses the growth and Motility Of lung cancer cells and has a significant correlation with the clinical outcome of the patients. These results contribute to our understanding of the molecular mechanisms of PEDF and indicates a potential prognostic and therapeutic impact of PEDF in lung cancer.

语种英语
WOS记录号WOS:000267231400018
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66347
专题北京大学临床肿瘤学院_胸外科
北京大学临床肿瘤学院_胸部肿瘤外二科
作者单位1.Cardiff Univ, Metastasis & Angiogenesis Res Grp, Dept Surg, Sch Med, Cardiff CF14 4XN, S Glam, Wales
2.Peking Univ, Key Lab Carcinogenesis & Translat Res, Dept Thorac Surg, Beijing Canc Hosp & Inst,Sch Oncol,Minist Educ, Beijing 100036, Peoples R China
推荐引用方式
GB/T 7714
Chen, Jinfeng,Ye, Lin,Zhang, Lijian,et al. The molecular impact of pigment epithelium-derived factor, PEDF, on lung cancer cells and the clinical significance[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2009,35(1):159-166.
APA Chen, Jinfeng,Ye, Lin,Zhang, Lijian,&Jiang, Wen G..(2009).The molecular impact of pigment epithelium-derived factor, PEDF, on lung cancer cells and the clinical significance.INTERNATIONAL JOURNAL OF ONCOLOGY,35(1),159-166.
MLA Chen, Jinfeng,et al."The molecular impact of pigment epithelium-derived factor, PEDF, on lung cancer cells and the clinical significance".INTERNATIONAL JOURNAL OF ONCOLOGY 35.1(2009):159-166.
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