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学科主题: 基础医学
题名:
Oxidative stress mediates CoCl2-induced prostate tumour cell adhesion: Role of protein kinase C and p38 mitogen-activated protein kinase
作者: Lu, Ning; Zhou, Hong; Lin, Yan-hua; Chen, Zhi-qiang; Pan, Yan; Li, Xue-jun
刊名: BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
发表日期: 2007-07-01
卷: 101, 期:1, 页:41-46
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Pharmacology & Pharmacy ; Toxicology
关键词[WOS]: HYPOXIA-INDUCIBLE FACTOR-1-ALPHA ; ERYTHROPOIETIN GENE ; INDUCED APOPTOSIS ; CANCER-THERAPY ; OXYGEN SENSOR ; HEME PROTEIN ; EXPRESSION ; INDUCTION ; COBALT ; HIF-1-ALPHA
英文摘要:

Cobalt chloride (CoCl2), an agent demonstrated to stabilize hypoxia-inducible factor-1, has been associated with various hypoxic responses, and recently, some reports have linked it to increasing tumour malignancy. In this study, we observed the alteration of cell adhesion after CoCl2 treatment and analysed the potential mechanisms responsible for such adaptations in a prostate cancer cell line PC-3 M cell. We found that CoCl2 increased the tumour cell adhesion in a dose-dependent manner, which correlated with reactive oxygen species (ROS) generation. When cells were incubated with the thiol reductive agent pyrrolidine dithiocarbamate (PDTC), both the ROS generation and the COCl2-induced cell adhesion were abolished. Moreover, p38 mitogen-activated protein kinase (p38 MAPK) was activated in COCl2-treated cells, which could be antagonized by PDTC. And when cells were pre-incubated with specific p38 MAPK inhibitor, SB203580, the cell adhesion induced by CoCl2 was diminished. Moreover, the protein kinase C could up-regulate cell adhesion through activating p38 MAPK. In conclusion, CoCl2 induced ROS generation, thereby placing cells under oxidative stress and up-regulating cell adhesion; p38 MAPK and protein kinase C could be activated in a ROS-dependent fashion, which in turn contributed to cell adhesion induced by CoCl2.

语种: 英语
WOS记录号: WOS:000247681900007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66354
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Peking Univ, Dept Pharmacol, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China

Recommended Citation:
Lu, Ning,Zhou, Hong,Lin, Yan-hua,et al. Oxidative stress mediates CoCl2-induced prostate tumour cell adhesion: Role of protein kinase C and p38 mitogen-activated protein kinase[J]. BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY,2007,101(1):41-46.
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