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学科主题: 药学
题名:
Quantification of myrislignan in rat plasma by solid-phase extraction and reversed-phase high-performance liquid chromatography
作者: Li, Fei; Yang, Xiuwei
关键词: Myristica fragrans ; myrislignan ; reversed-phase HPLC ; solid-phase extraction ; pharmacokinetics
刊名: BIOMEDICAL CHROMATOGRAPHY
发表日期: 2008-06-01
DOI: 10.1002/bmc.975
卷: 22, 期:6, 页:601-605
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: NUTMEG
英文摘要:

A rapid and simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for determination of myrislignan in rat plasma after intravenous administration. The analytes extracted from plasma samples by solid-phase extraction were successfully carried out on a Diamonsil (TM) ODS C-18 column (250 x 4.6 mm i.d., 5 mu m) with an RP18 guard column (8 x 4.6 mm id., 5 mu m) and a mobile phase of MeOH-H2O (4:1, v/v). The UV detector was set at a single wave-length of 270 nm. The linear ranges of the standard curves were 0.5-30.0 mu g/mL with the correlation coefficients greater than 0.9992. The lower limits of detection and quantification were 0.1 and 0.3 mu g/mL for myrislignan. Intra- and inter-day precisions were 2.4-7.5 and 1.3-5.7%, respectively. The extraction recovery from plasma was more than 90%. This assay method has been successfully used to study the pharmacokinetics of myrislignan in rats. Copyright (C) 2008 John Wiley & Sons, Ltd.

语种: 英语
WOS记录号: WOS:000256875200007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66357
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China

Recommended Citation:
Li, Fei,Yang, Xiuwei. Quantification of myrislignan in rat plasma by solid-phase extraction and reversed-phase high-performance liquid chromatography[J]. BIOMEDICAL CHROMATOGRAPHY,2008,22(6):601-605.
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