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Synaptotagmin1 is required for spindle stability and metaphase-to-anaphase transition in mouse oocytes
Zhu, Xiu-Lan1,2; Qi, Shu-Tao2; Liu, Jun1; Chen, Lei2; Zhang, Chun-Hui3; Yang, Shang-Wu1,2; Ouyang, Ying-Chun2; Hou, Yi2; Schatten, Heide4; Song, Ya-Li1; Xing, Fu-Qi1; Sun, Qing-Yuan2
关键词synaptotagmin1 mouse oocyte meiosis MTOC spindle metaphase-anaphase transition
刊名CELL CYCLE
2012-02-15
DOI10.4161/cc.11.4.19329
11期:4页:818-826
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]ACCURATE CHROMOSOME SEGREGATION ; CORTICAL GRANULE EXOCYTOSIS ; CELL-CYCLE PROGRESSION ; POLO-LIKE KINASE-1 ; SEA-URCHIN ; ASYMMETRIC DIVISION ; MEIOTIC MATURATION ; PROTEIN ; ORGANIZATION ; CENTROSOME
英文摘要

Synaptotagmin1, a calcium sensor for exocytosis, forms the 7S complex, or so-called SNARE protein complex, together with SNAP-25, syntaxin and synaptobrevin to mediate docking and fusion of synaptic vesicles to the plasma membrane of the nerve terminal. Here, we identified the unique localization, expression and function of Syt1 during mouse oocyte meiotic maturation by using confocal microscopy, western blotting, Morpholino-based knockdown and time-lapse live cell imaging. We showed that Syt1 expression was gradually increased during oocyte maturation. Syt1 was localized at the oocyte cortex from GV to MII stages and at the spindle poles in MI and MII phases, with one third of a signal-free zone at the oocyte cortex, where the chromosomes are located, which is similar to the distribution pattern of CGs from the pro-MI to MII stages. Knockdown of Syt1 resulted in pro-MI/MI arrest and PB1 extrusion decrease, with severely disrupted spindles and misaligned chromosomes. Knockdown of Syt1 also caused abnormal localization of gamma-tubulin, which became redistributed into the cytoplasm. Chromosome spreading showed failure of homologous chromosome segregation. The spindle assembly checkpoint protein Bub3 was detected at the kinetochores even after 10 h of oocyte culture. Live cell imaging analysis revealed that knockdown of Syt1 resulted in abnormal spindles with various morphologies and chromosomes arrested at the pro-MI/MI stage. Defective spindles failed to support chromosome alignment along microtubules, which led to repetitive unsuccessful metaphase-anaphase transitions and failure of PB1 extrusion after extended culture. Taken together, we suggest that Syt1 may act as a MTOC-associated protein to play important roles in mouse oocyte spindle organization/stability, and that it is indispensable for the metaphase-anaphase transition to promote mouse oocyte meiotic maturation.

语种英语
WOS记录号WOS:000300621400032
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66358
专题北京大学深圳医院
作者单位1.So Med Univ, Ctr Reprod Med, Dept Ob Gy, Nanfang Hosp, Guangzhou, Guangdong, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
3.Peking Univ, Dept Reprod Med, Shenzhen Hosp, Med Ctr, Shenzhen, Guangdong, Peoples R China
4.Univ Missouri, Dept Vet Pathobiol, Columbia, MO USA
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Zhu, Xiu-Lan,Qi, Shu-Tao,Liu, Jun,et al. Synaptotagmin1 is required for spindle stability and metaphase-to-anaphase transition in mouse oocytes[J]. CELL CYCLE,2012,11(4):818-826.
APA Zhu, Xiu-Lan.,Qi, Shu-Tao.,Liu, Jun.,Chen, Lei.,Zhang, Chun-Hui.,...&Sun, Qing-Yuan.(2012).Synaptotagmin1 is required for spindle stability and metaphase-to-anaphase transition in mouse oocytes.CELL CYCLE,11(4),818-826.
MLA Zhu, Xiu-Lan,et al."Synaptotagmin1 is required for spindle stability and metaphase-to-anaphase transition in mouse oocytes".CELL CYCLE 11.4(2012):818-826.
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