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学科主题: 临床医学
题名:
Synaptotagmin1 is required for spindle stability and metaphase-to-anaphase transition in mouse oocytes
作者: Zhu, Xiu-Lan1,2; Qi, Shu-Tao2; Liu, Jun1; Chen, Lei2; Zhang, Chun-Hui3; Yang, Shang-Wu1,2; Ouyang, Ying-Chun2; Hou, Yi2; Schatten, Heide4; Song, Ya-Li1; Xing, Fu-Qi1; Sun, Qing-Yuan2
关键词: synaptotagmin1 ; mouse oocyte meiosis ; MTOC ; spindle ; metaphase-anaphase transition
刊名: CELL CYCLE
发表日期: 2012-02-15
DOI: 10.4161/cc.11.4.19329
卷: 11, 期:4, 页:818-826
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: ACCURATE CHROMOSOME SEGREGATION ; CORTICAL GRANULE EXOCYTOSIS ; CELL-CYCLE PROGRESSION ; POLO-LIKE KINASE-1 ; SEA-URCHIN ; ASYMMETRIC DIVISION ; MEIOTIC MATURATION ; PROTEIN ; ORGANIZATION ; CENTROSOME
英文摘要:

Synaptotagmin1, a calcium sensor for exocytosis, forms the 7S complex, or so-called SNARE protein complex, together with SNAP-25, syntaxin and synaptobrevin to mediate docking and fusion of synaptic vesicles to the plasma membrane of the nerve terminal. Here, we identified the unique localization, expression and function of Syt1 during mouse oocyte meiotic maturation by using confocal microscopy, western blotting, Morpholino-based knockdown and time-lapse live cell imaging. We showed that Syt1 expression was gradually increased during oocyte maturation. Syt1 was localized at the oocyte cortex from GV to MII stages and at the spindle poles in MI and MII phases, with one third of a signal-free zone at the oocyte cortex, where the chromosomes are located, which is similar to the distribution pattern of CGs from the pro-MI to MII stages. Knockdown of Syt1 resulted in pro-MI/MI arrest and PB1 extrusion decrease, with severely disrupted spindles and misaligned chromosomes. Knockdown of Syt1 also caused abnormal localization of gamma-tubulin, which became redistributed into the cytoplasm. Chromosome spreading showed failure of homologous chromosome segregation. The spindle assembly checkpoint protein Bub3 was detected at the kinetochores even after 10 h of oocyte culture. Live cell imaging analysis revealed that knockdown of Syt1 resulted in abnormal spindles with various morphologies and chromosomes arrested at the pro-MI/MI stage. Defective spindles failed to support chromosome alignment along microtubules, which led to repetitive unsuccessful metaphase-anaphase transitions and failure of PB1 extrusion after extended culture. Taken together, we suggest that Syt1 may act as a MTOC-associated protein to play important roles in mouse oocyte spindle organization/stability, and that it is indispensable for the metaphase-anaphase transition to promote mouse oocyte meiotic maturation.

语种: 英语
所属项目编号: 2012CB944404 ; 2011CB944501 ; 30930065
项目资助者: Major Basic Research Program ; National Natural Science Foundation of China
WOS记录号: WOS:000300621400032
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66358
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.So Med Univ, Ctr Reprod Med, Dept Ob Gy, Nanfang Hosp, Guangzhou, Guangdong, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing, Peoples R China
3.Peking Univ, Dept Reprod Med, Shenzhen Hosp, Med Ctr, Shenzhen, Guangdong, Peoples R China
4.Univ Missouri, Dept Vet Pathobiol, Columbia, MO USA

Recommended Citation:
Zhu, Xiu-Lan,Qi, Shu-Tao,Liu, Jun,et al. Synaptotagmin1 is required for spindle stability and metaphase-to-anaphase transition in mouse oocytes[J]. CELL CYCLE,2012,11(4):818-826.
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