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The permeability and cytotoxicity of insulin-mimetic vanadium compounds
Yang, XG; Yang, XD; Yuan, L; Wang, K; Crans, DC
关键词Caco-2 cells confocal laser scanning microscopy cytotoxicity F-actin vanadium compounds
刊名PHARMACEUTICAL RESEARCH
2004-06-01
21期:6页:1026-1033
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]CACO-2 CELLS ; DIABETES-MELLITUS ; MODEL SYSTEM ; IN-VIVO ; CHEMISTRY ; TRANSPORT ; COMPLEXES ; MONOLAYERS ; TOXICITY ; SULFATE
英文摘要

Purpose. The aim of this study was to investigate the mechanism of permeation and cytotoxicity of vanadium compounds, [VO(acac)(2)], [VO(ma)(2)], and vanadate.

Methods. Absorptive transport were carried out in Caco-2 monolayers grown on transwell inserts. Vanadium was quantified using inductively coupled plasma atomic emission spectrometry (ICP-AES). The change of Caco-2 cells in the microvilli morphology and F-actin structure was visualized by transmission electron microscopy and confocal laser scanning microscopy.

Results. The three vanadium compounds were taken up by Caco-2 cells via simple passive diffusion. [VO(acac)(2)] were mainly transcellularly transported and exhibited the highest apparent permeabilty coefficients (8.2 x 10(-6) cm(-1)). The cell accumulation of [VO(acac)(2)] was found to be greater than that of [VO(ma)(2)], and vanadate caused much less accumulation than the other two compounds. Vanadium compounds induced intracellular reactive oxygen species, reduced the transepithelial electric resistance, caused morphological change in microvilli, and led to different perturbation of F-actin structure.

Conclusions. The three compounds exhibited different permeability due to different diffusion process and cellular uptake. The toxicity of vanadium complexes on Caco-2 monolayer involved F-actin-related change of tight junction and impairment of microvilli. The toxicity was also related to elevated intracellular reactive oxygen species (ROS) and their cellular accumulation.

语种英语
WOS记录号WOS:000221685800019
引用统计
被引频次:75[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66372
专题北京大学药学院_化学生物学系
作者单位1.Colorado State Univ, Dept Chem, Ft Collins, CO 80523 USA
2.Peking Univ, Analyt Ctr, Beijing 100083, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
4.Colorado State Univ, Coll Nat Sci, Program Mol Biol, Ft Collins, CO 80523 USA
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GB/T 7714
Yang, XG,Yang, XD,Yuan, L,et al. The permeability and cytotoxicity of insulin-mimetic vanadium compounds[J]. PHARMACEUTICAL RESEARCH,2004,21(6):1026-1033.
APA Yang, XG,Yang, XD,Yuan, L,Wang, K,&Crans, DC.(2004).The permeability and cytotoxicity of insulin-mimetic vanadium compounds.PHARMACEUTICAL RESEARCH,21(6),1026-1033.
MLA Yang, XG,et al."The permeability and cytotoxicity of insulin-mimetic vanadium compounds".PHARMACEUTICAL RESEARCH 21.6(2004):1026-1033.
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