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Potential transcriptional regulatory regions exist upstream of the human ezrin gene promoter in esophageal carcinoma cells
Gao, Shuying1; Dai, Yanpeng1; Yin, Meijun1; Ye, Jing1; Li, Gang2; Yu, Jie1,3
关键词ezrin VIL2 promoter enhancer esophageal carcinoma
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
2011-06-01
DOI10.1093/abbs/gmr033
43期:6页:455-464
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]CROSS-LINKER EZRIN ; EPITHELIAL-CELLS ; SV40 ENHANCER ; HIGH-LEVEL ; EXPRESSION ; METASTASIS ; PROTEIN ; ADENOCARCINOMA ; PHENOTYPE ; PATHWAY
英文摘要

We previously demonstrated that the region -87/+134 of the human ezrin gene (VIL2) exhibited promoter activity in human esophageal carcinoma EC109 cells, and a further upstream region -1324/-890 positively regulated transcription. In this study, to identify the transcriptional regulatory regions upstream of the VIL2 promoter, we cloned VIL2 -1541/-706 segment containing the -1324/-890, and investigated its transcriptional regulatory properties via luciferase assays in transiently transfected cells. In EC109 cells, it was found that VIL2 -1541/-706 possessed promoter and enhancer activities. We also localized transcriptional regulatory regions by fusing 5′- or 3′-deletion segments of VIL2 -1541/-706 to a luciferase reporter. We found that there were three positive and one negative transcriptional regulatory regions within VIL2 -1541/-706 in EC109 cells. When these regions were separately located upstream of the luciferase gene without promoter, or located upstream of the VIL2 promoter or SV40 promoter directing the luciferase gene, only VIL2 -1297/-1186 exhibited considerable promoter and enhancer activities, which were lower than those of -1541/-706. In addition, transient expression of Sp1 increased ezrin expression and the transcriptional activation of VIL2 -1297/-1186. Other three regions, although exhibiting significantly positive or negative transcriptional regulation in deletion experiments, showed a weaker or absent regulation. These data suggested that more than one region upstream of the VIL2 promoter participated in VIL2 transcription, and the VIL2 -1297/-1186, probably as a key transcriptional regulatory region, regulated VIL2 transcription in company with other potential regulatory regions.

语种英语
WOS记录号WOS:000291258100005
项目编号20090450100 ; 201003361 ; 9152800001000017 ; 9151030002000008 ; JC200903180676A ; 200901012 ; 200801001
资助机构China Postdoctoral Science Foundation ; Natural Science Foundation of Guangdong Province ; Basic Research Projects of Shenzhen Bureau of Science, Technology, and Information
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66376
专题北京大学深圳医院_中心实验室
北京大学基础医学院
北京大学第三临床医学院_胸外科
作者单位1.Peking Univ, Shenzhen Hosp, Cent Lab, Shenzhen PKU HKUST Med Ctr, Shenzhen 518036, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 10081, Peoples R China
3.Peking Univ, Shenzhen Hosp, Lab Male Reprod, Shenzhen PKU HKUST Med Ctr, Shenzhen 518036, Peoples R China
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Gao, Shuying,Dai, Yanpeng,Yin, Meijun,et al. Potential transcriptional regulatory regions exist upstream of the human ezrin gene promoter in esophageal carcinoma cells[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2011,43(6):455-464.
APA Gao, Shuying,Dai, Yanpeng,Yin, Meijun,Ye, Jing,Li, Gang,&Yu, Jie.(2011).Potential transcriptional regulatory regions exist upstream of the human ezrin gene promoter in esophageal carcinoma cells.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,43(6),455-464.
MLA Gao, Shuying,et al."Potential transcriptional regulatory regions exist upstream of the human ezrin gene promoter in esophageal carcinoma cells".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 43.6(2011):455-464.
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