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学科主题: 临床医学
题名:
Potential transcriptional regulatory regions exist upstream of the human ezrin gene promoter in esophageal carcinoma cells
作者: Gao, Shuying1; Dai, Yanpeng1; Yin, Meijun1; Ye, Jing1; Li, Gang2; Yu, Jie1,3
关键词: ezrin ; VIL2 ; promoter ; enhancer ; esophageal carcinoma
刊名: ACTA BIOCHIMICA ET BIOPHYSICA SINICA
发表日期: 2011-06-01
DOI: 10.1093/abbs/gmr033
卷: 43, 期:6, 页:455-464
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: CROSS-LINKER EZRIN ; EPITHELIAL-CELLS ; SV40 ENHANCER ; HIGH-LEVEL ; EXPRESSION ; METASTASIS ; PROTEIN ; ADENOCARCINOMA ; PHENOTYPE ; PATHWAY
英文摘要:

We previously demonstrated that the region -87/+134 of the human ezrin gene (VIL2) exhibited promoter activity in human esophageal carcinoma EC109 cells, and a further upstream region -1324/-890 positively regulated transcription. In this study, to identify the transcriptional regulatory regions upstream of the VIL2 promoter, we cloned VIL2 -1541/-706 segment containing the -1324/-890, and investigated its transcriptional regulatory properties via luciferase assays in transiently transfected cells. In EC109 cells, it was found that VIL2 -1541/-706 possessed promoter and enhancer activities. We also localized transcriptional regulatory regions by fusing 5′- or 3′-deletion segments of VIL2 -1541/-706 to a luciferase reporter. We found that there were three positive and one negative transcriptional regulatory regions within VIL2 -1541/-706 in EC109 cells. When these regions were separately located upstream of the luciferase gene without promoter, or located upstream of the VIL2 promoter or SV40 promoter directing the luciferase gene, only VIL2 -1297/-1186 exhibited considerable promoter and enhancer activities, which were lower than those of -1541/-706. In addition, transient expression of Sp1 increased ezrin expression and the transcriptional activation of VIL2 -1297/-1186. Other three regions, although exhibiting significantly positive or negative transcriptional regulation in deletion experiments, showed a weaker or absent regulation. These data suggested that more than one region upstream of the VIL2 promoter participated in VIL2 transcription, and the VIL2 -1297/-1186, probably as a key transcriptional regulatory region, regulated VIL2 transcription in company with other potential regulatory regions.

语种: 英语
所属项目编号: 20090450100 ; 201003361 ; 9152800001000017 ; 9151030002000008 ; JC200903180676A ; 200901012 ; 200801001
项目资助者: China Postdoctoral Science Foundation ; Natural Science Foundation of Guangdong Province ; Basic Research Projects of Shenzhen Bureau of Science, Technology, and Information
WOS记录号: WOS:000291258100005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66376
Appears in Collections:北京大学深圳医院_中心实验室_期刊论文

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作者单位: 1.Peking Univ, Shenzhen Hosp, Cent Lab, Shenzhen PKU HKUST Med Ctr, Shenzhen 518036, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, Beijing 10081, Peoples R China
3.Peking Univ, Shenzhen Hosp, Lab Male Reprod, Shenzhen PKU HKUST Med Ctr, Shenzhen 518036, Peoples R China

Recommended Citation:
Gao, Shuying,Dai, Yanpeng,Yin, Meijun,et al. Potential transcriptional regulatory regions exist upstream of the human ezrin gene promoter in esophageal carcinoma cells[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2011,43(6):455-464.
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