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学科主题基础医学
Characterization of kinesin-like proteins in silkworm posterior silkgland cells
Wang, Qiao1; Teng, Junlin1; Shen, Birong1; Zhang, Wei1; Guo, Yige1; Su, Xiaolei1; Zhang, Chuanxi3; Yu, Albert C. H.4,5; Chen, Jianguo1,2
关键词Kinesin-like Proteins Posterior Silkgland Cells Intracellular Transport Exuperantia Fibroin Granules Bmkinesin-1
刊名CELL RESEARCH
2010-06-01
DOI10.1038/cr.2010.47
20期:6页:713-727
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]MESSENGER-RNA LOCALIZATION ; BOMBYX-MORI ; SEQUENCE ALIGNMENT ; AXONAL-TRANSPORT ; MOLECULAR MOTORS ; MICROTUBULE ENDS ; GLAND CELLS ; FIBROIN ; IDENTIFICATION ; SUPERFAMILY
英文摘要

Kinesins are microtubule-based motors involved in various intracellular transports. Neurons, flagellated cells, and pigment cells have been traditionally used as model systems to study the cellular functions of kinesins. Here, we report silkworm posterior silkgland (PSG), specialized cells with an extensive endomembrane system for intracellular transport and efficient secretion of fibroin, as a novel model for kinesin study. To investigate kinesin-driven intracellular transport in PSG cells, we cloned five silkworm kinesin-like proteins (KLPs), BmKinesin-1, BmKinesin-6, BmKinesin-7, BmKinesin-13, and BmKinesin-14A. We determined their expression patterns by relative real-time PCR and western blotting. Immunofluorescence microscopy verified their colocalization with microtubules. By combining pull-down assays, LC-MS/MS, and western blotting analysis, we identified many potential cargoes of BmKinesin-1 in PSG, including fibroin-containing granules and exuperantia-associated ribonucleoprotein (RNP) complexes. Moreover, BmKinesin-13 overexpression disrupted the microtubule network in BmN cells, which is consistent with a role of Kinesin-13 in regulating microtubule dynamics in other organisms. On the basis of these results, we concluded that PSG might have advantages in elucidating mechanisms of intracellular transport in secretory tissues and could serve as a potential model for kinesin studies.

语种英语
WOS记录号WOS:000278726600013
项目编号0670659 ; 30771086 ; 30721064 ; 2006CB500700 ; 2006CB910700 ; 2010CB833705 ; 2006AA10A119
资助机构National Natural Science Foundation of China ; Major State Basic Research Development Program of China (973 Program) ; National High Technology Research and Development Program of China (863 Program)
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66386
专题北京大学基础医学院_神经生物学系
北京大学口腔医学院_义齿加工中心
作者单位1.Peking Univ, Coll Life Sci, State Key Lab Biomembrane & Membrane Bioengn, Key Lab Cell Proliferat & Differentiat,Minist Edu, Beijing 100871, Peoples R China
2.Zhejiang Univ, Inst Insect Sci, Hangzhou 310029, Zhejiang, Peoples R China
3.Peking Univ, Dept Neurobiol, Neurosci Res Inst, Sch Basic Med Sci,Key Lab Neurosci,Minist Educ, Beijing 100191, Peoples R China
4.Peking Univ, Dept Neurobiol, Neurosci Res Inst, Sch Basic Med Sci,Key Lab Neurosci,Minist Publ Hl, Beijing 100191, Peoples R China
5.Peking Univ, Ctr Theoret Biol, Beijing 100871, Peoples R China
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GB/T 7714
Wang, Qiao,Teng, Junlin,Shen, Birong,et al. Characterization of kinesin-like proteins in silkworm posterior silkgland cells[J]. CELL RESEARCH,2010,20(6):713-727.
APA Wang, Qiao.,Teng, Junlin.,Shen, Birong.,Zhang, Wei.,Guo, Yige.,...&Chen, Jianguo.(2010).Characterization of kinesin-like proteins in silkworm posterior silkgland cells.CELL RESEARCH,20(6),713-727.
MLA Wang, Qiao,et al."Characterization of kinesin-like proteins in silkworm posterior silkgland cells".CELL RESEARCH 20.6(2010):713-727.
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