学科主题临床医学
Hepatic differentiation capability of rat bone marrow-derived mesenchymal stem cells and hematopoietic stem cells
Shu, Sai-Nan1; Wei, Lai1; Wang, Jiang-Hua1; Zhan, Yu-Tao2; Chen, Hong-Song1; Wang, Yu1
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2004-10-01
10期:19页:2818-2822
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
英文摘要

AIM: To investigate the different effects of mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) on hepatic differentiation.

METHODS: MSCs from rat bone marrow were isolated and cultured by standard methods. HSCs from rat bone marrow were isolated and purified by magnetic activated cell sorting. Both cell subsets were induced. Morphology, RT-PCR and immunocytochemistry were used to identify the hepatic differentiation grade.

RESULTS: MSCs exhibited round in shape after differentiation, instead of fibroblast-like morphology before differentiation. Albumin mRNA and protein were expressed positively in MSCs, without detection of alpha-fetoprotein (AFP). HSCs were polygonal in shape after differentiation. The expression of albumin signal decreased and AFP signal increased. The expression of CK18 was continuous in MSCs and HSCs both before and after induction.

CONCLUSION: Both MSCs and HSCs have hepatic differentiation capabilities. However, their capabilities are not the same. MSCs can differentiate into mature hepatocyte-like cells, never expressing early hepatic specific genes, while Thy-1.1(+) cells are inclined to differentiate into hepatic stem cell-like cells, with an increasing AFP expression and a decreasing albumin signal. CK18 mRNA is positive in Thy-1.1(+) cells and MSCs, negative in Thy-1.1(-) cells. It seems that CK18 has some relationship with Thy-1.1 antigen, and CK18 may be a predictive marker of hepatic differentiation capability.

语种英语
WOS记录号WOS:000208097300011
项目编号2001AA216031
资助机构National High Technology Research and Development Program of China (863 Program)
引用统计
被引频次:55[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66395
专题北京大学第二临床医学院_北京大学肝病研究所
作者单位1.Peking Univ, Inst Hepatol, Peoples Hosp, Beijing 100044, Peoples R China
2.Capital Med Univ, Dept Gastroenterol, Tong Ren Hosp, Beijing 100730, Peoples R China
推荐引用方式
GB/T 7714
Shu, Sai-Nan,Wei, Lai,Wang, Jiang-Hua,et al. Hepatic differentiation capability of rat bone marrow-derived mesenchymal stem cells and hematopoietic stem cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(19):2818-2822.
APA Shu, Sai-Nan,Wei, Lai,Wang, Jiang-Hua,Zhan, Yu-Tao,Chen, Hong-Song,&Wang, Yu.(2004).Hepatic differentiation capability of rat bone marrow-derived mesenchymal stem cells and hematopoietic stem cells.WORLD JOURNAL OF GASTROENTEROLOGY,10(19),2818-2822.
MLA Shu, Sai-Nan,et al."Hepatic differentiation capability of rat bone marrow-derived mesenchymal stem cells and hematopoietic stem cells".WORLD JOURNAL OF GASTROENTEROLOGY 10.19(2004):2818-2822.
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