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学科主题: 临床医学
题名:
Hepatic differentiation capability of rat bone marrow-derived mesenchymal stem cells and hematopoietic stem cells
作者: Shu, Sai-Nan1; Wei, Lai1; Wang, Jiang-Hua1; Zhan, Yu-Tao2; Chen, Hong-Song1; Wang, Yu1
刊名: WORLD JOURNAL OF GASTROENTEROLOGY
发表日期: 2004-10-01
卷: 10, 期:19, 页:2818-2822
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
英文摘要:

AIM: To investigate the different effects of mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) on hepatic differentiation.

METHODS: MSCs from rat bone marrow were isolated and cultured by standard methods. HSCs from rat bone marrow were isolated and purified by magnetic activated cell sorting. Both cell subsets were induced. Morphology, RT-PCR and immunocytochemistry were used to identify the hepatic differentiation grade.

RESULTS: MSCs exhibited round in shape after differentiation, instead of fibroblast-like morphology before differentiation. Albumin mRNA and protein were expressed positively in MSCs, without detection of alpha-fetoprotein (AFP). HSCs were polygonal in shape after differentiation. The expression of albumin signal decreased and AFP signal increased. The expression of CK18 was continuous in MSCs and HSCs both before and after induction.

CONCLUSION: Both MSCs and HSCs have hepatic differentiation capabilities. However, their capabilities are not the same. MSCs can differentiate into mature hepatocyte-like cells, never expressing early hepatic specific genes, while Thy-1.1(+) cells are inclined to differentiate into hepatic stem cell-like cells, with an increasing AFP expression and a decreasing albumin signal. CK18 mRNA is positive in Thy-1.1(+) cells and MSCs, negative in Thy-1.1(-) cells. It seems that CK18 has some relationship with Thy-1.1 antigen, and CK18 may be a predictive marker of hepatic differentiation capability.

语种: 英语
所属项目编号: 2001AA216031
项目资助者: National High Technology Research and Development Program of China (863 Program)
WOS记录号: WOS:000208097300011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66395
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: 1.Peking Univ, Inst Hepatol, Peoples Hosp, Beijing 100044, Peoples R China
2.Capital Med Univ, Dept Gastroenterol, Tong Ren Hosp, Beijing 100730, Peoples R China

Recommended Citation:
Shu, Sai-Nan,Wei, Lai,Wang, Jiang-Hua,et al. Hepatic differentiation capability of rat bone marrow-derived mesenchymal stem cells and hematopoietic stem cells[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(19):2818-2822.
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