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学科主题基础医学
The protein profile of acetazolamide-treated sera in mice bearing Lewis neoplasm
Xiang, Y; Ma, B; Yu, HM; Li, XJ
关键词neoplasm acetazolamide proteomics
刊名LIFE SCIENCES
2004-07-30
DOI10.1016/j.lfs.2003.12.032
75期:11页:1277-1285
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]CELL-LINES ; IDENTIFICATION ; EXPRESSION ; DIFFERENTIATION ; ELECTROPHORESIS ; MARKERS ; DEFINE
英文摘要

The aim of the present research is to analyze the protcome of neoplasm serum before and after treated with acetazolamide (20, 40, 80 mg kg(-1) d(-1) for 3 days p.o.). The Lewis lung carcinoma mice were used and carried out a comprehensive proteomic analysis by using the technologies of high-resolution two-dimensional polyacrylamide gel electrophoresis (21) PAGE) and mass spectrometry (MS). The results showed that the acetazolamide could dramatically reduce the lung metastasis and primary tumor growth. Its most potent inhibition rate on lung metastases was reach to 77.7% at the dose of 80 mg kg(-1) d(-1). The two dimension electrophoresis and software analysis reveal 393 protein spots in control gel, 385 protein spots were detected in treated gel and matched 209 protein spots with control gel, indicating that intensive changes had occurred during the process of treatment. Two obviously different spots were cut off from gel and for the peptide mass fingerprinting. Data base searching showed the two proteins′ peptide much more mach with Historic H2B fragment and Ubc-like protein CROC1 fragment. The results suggest that acetazolamide has a strong anti-tumor and anti-metastasis effect on Lewis-lung-carcinoma. The mechanism may be related to its regulation on plenty of proteins, in particular, on upregulation of H2B and CROC-I expression of postreplicational DNA repair related protein in serum. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000222575500001
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66401
专题北京大学基础医学院_药理学系
作者单位1.Peking Univ, Dept Pharmacol, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Natl Res Inst Family Planning, Beijing 100081, Peoples R China
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GB/T 7714
Xiang, Y,Ma, B,Yu, HM,et al. The protein profile of acetazolamide-treated sera in mice bearing Lewis neoplasm[J]. LIFE SCIENCES,2004,75(11):1277-1285.
APA Xiang, Y,Ma, B,Yu, HM,&Li, XJ.(2004).The protein profile of acetazolamide-treated sera in mice bearing Lewis neoplasm.LIFE SCIENCES,75(11),1277-1285.
MLA Xiang, Y,et al."The protein profile of acetazolamide-treated sera in mice bearing Lewis neoplasm".LIFE SCIENCES 75.11(2004):1277-1285.
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