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学科主题: 基础医学
题名:
The protein profile of acetazolamide-treated sera in mice bearing Lewis neoplasm
作者: Xiang, Y; Ma, B; Yu, HM; Li, XJ
关键词: neoplasm ; acetazolamide ; proteomics
刊名: LIFE SCIENCES
发表日期: 2004-07-30
DOI: 10.1016/j.lfs.2003.12.032
卷: 75, 期:11, 页:1277-1285
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: CELL-LINES ; IDENTIFICATION ; EXPRESSION ; DIFFERENTIATION ; ELECTROPHORESIS ; MARKERS ; DEFINE
英文摘要:

The aim of the present research is to analyze the protcome of neoplasm serum before and after treated with acetazolamide (20, 40, 80 mg kg(-1) d(-1) for 3 days p.o.). The Lewis lung carcinoma mice were used and carried out a comprehensive proteomic analysis by using the technologies of high-resolution two-dimensional polyacrylamide gel electrophoresis (21) PAGE) and mass spectrometry (MS). The results showed that the acetazolamide could dramatically reduce the lung metastasis and primary tumor growth. Its most potent inhibition rate on lung metastases was reach to 77.7% at the dose of 80 mg kg(-1) d(-1). The two dimension electrophoresis and software analysis reveal 393 protein spots in control gel, 385 protein spots were detected in treated gel and matched 209 protein spots with control gel, indicating that intensive changes had occurred during the process of treatment. Two obviously different spots were cut off from gel and for the peptide mass fingerprinting. Data base searching showed the two proteins′ peptide much more mach with Historic H2B fragment and Ubc-like protein CROC1 fragment. The results suggest that acetazolamide has a strong anti-tumor and anti-metastasis effect on Lewis-lung-carcinoma. The mechanism may be related to its regulation on plenty of proteins, in particular, on upregulation of H2B and CROC-I expression of postreplicational DNA repair related protein in serum. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000222575500001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66401
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Peking Univ, Dept Pharmacol, Sch Basic Med Sci, Beijing 100083, Peoples R China
2.Natl Res Inst Family Planning, Beijing 100081, Peoples R China

Recommended Citation:
Xiang, Y,Ma, B,Yu, HM,et al. The protein profile of acetazolamide-treated sera in mice bearing Lewis neoplasm[J]. LIFE SCIENCES,2004,75(11):1277-1285.
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