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学科主题临床医学
High-efficiency cell-substrate displacement acquisition via digital image correlation method using basis functions
Huang, Jianyong1,2; Pan, Xiaochang1,2; Peng, Xiaoling1,2; Zhu, Tao1,2; Qin, Lei2,3; Xiong, Chunyang1,2; Fang, Jing1,2
关键词Cellular deformation measurement Cellular traction force microscope Digital image processing Fast normalized cross correlation
刊名OPTICS AND LASERS IN ENGINEERING
2010-11-01
DOI10.1016/j.optlaseng.2009.12.009
48期:11页:1058-1066
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Optics
资助者National Basic Research Program ; NSFC ; National Basic Research Program ; NSFC
研究领域[WOS]Optics
关键词[WOS]NORMALIZED CROSS-CORRELATION ; TRACTION FORCE MICROSCOPY ; REGISTRATION ALGORITHMS ; GENERATED DEFORMATIONS ; FLEXIBLE SUBSTRATA ; FOCAL ADHESIONS ; FIBROBLASTS ; LOCOMOTION ; MECHANICS ; FIELDS
英文摘要

Cellular traction force microscopy (CTFM) has become an emerging technique to investigate spatiotemporal dynamics of cell-substrate interaction The displacement field acquisition in CTFM, however, is essentially quite time-consuming due to the computational complexity using digital image correlation (DIC) method. Addressing the intrinsic feature of substrate fluorescent images, this paper introduces the concept of basis function to simplify the zero-normalized cross-correlation (ZNCC) expression used in the conventional DIC calculation. By approximating fluorescent particles inside single reference subsets as a series of basis functions, all the terms in the modified ZNCC formula can be efficiently evaluated with the help of a sum-table approach The effectiveness and efficiency of the proposed fast algorithm are verified through simulations and actual cell-substrate displacement computations, which indicate that the integer-pixel correlation calculation based upon the new algorithm is about 10-20 times faster than that founded upon the conventional DIC algorithm where the ZNCC computation is performed in a straightforward way. (C) 2009 Elsevier Ltd. All rights reserved.

语种英语
所属项目编号2007CB935602 ; 90607004 ; 10672005 ; 10872008
资助者National Basic Research Program ; NSFC ; National Basic Research Program ; NSFC
WOS记录号WOS:000281505800003
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66402
Collection北京大学第三临床医学院_心血管内科
作者单位1.Peking Univ, Dept Biomed Engn, Beijing 100871, Peoples R China
2.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
3.Peking Univ, Inst Vasc Med, Hosp 3, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Huang, Jianyong,Pan, Xiaochang,Peng, Xiaoling,et al. High-efficiency cell-substrate displacement acquisition via digital image correlation method using basis functions[J]. OPTICS AND LASERS IN ENGINEERING,2010,48(11):1058-1066.
APA Huang, Jianyong.,Pan, Xiaochang.,Peng, Xiaoling.,Zhu, Tao.,Qin, Lei.,...&Fang, Jing.(2010).High-efficiency cell-substrate displacement acquisition via digital image correlation method using basis functions.OPTICS AND LASERS IN ENGINEERING,48(11),1058-1066.
MLA Huang, Jianyong,et al."High-efficiency cell-substrate displacement acquisition via digital image correlation method using basis functions".OPTICS AND LASERS IN ENGINEERING 48.11(2010):1058-1066.
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