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Genetic polymorphism of medicinally-used Codonopsis species in an internal transcribed spacer sequence of nuclear ribosomal DNA and its application to authenticate Codonopsis Radix
He, Jing Yu1; Zhu, Shu1; Komatsu, Katsuko1; Goda, Yukihiro2; Cai, Shao-Qing3
关键词Codonopsis Genetic polymorphism Internal transcribed spacer Molecular identification
刊名JOURNAL OF NATURAL MEDICINES
2014
DOI10.1007/s11418-013-0780-1
68期:1页:112-124
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Medicinal ; Pharmacology & Pharmacy
资助者JSPS KAKENHI ; Japan Health Sciences Foundation ; Expansion Program, Regional Innovation Cluster Program, Global Type (II) from MEXT, Japan ; JSPS KAKENHI ; Japan Health Sciences Foundation ; Expansion Program, Regional Innovation Cluster Program, Global Type (II) from MEXT, Japan
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]NUCLEOTIDE-SEQUENCE ; RDNA ; DIVERSITY ; REGION ; PLANTS
英文摘要

Codonopsis Radix has been prescribed as the roots of Codonopsis pilosula, C. pilosula var. modesta and C. tangshen in Chinese Pharmacopoeia. In order to find out genetic markers for identifying the 3 taxa and to authenticate Codonopsis Radix, the molecular analysis of the internal transcribed spacer sequence of nuclear ribosomal DNA was conducted on Codonopsis plants collected widely from Gansu Prov. and Chongqing city of China, the main producing areas of Codonopsis Radix. Significant genetic polymorphism was observed, represented by 11 types of ITS sequences in C. pilosula, 5 types in C. pilosula var. modesta and 5 types in C. tangshen. Among the determined sequences, 1, 1 and 2 types were thought to be of pure lines of each taxon, respectively, designated as types P0, PM0, T1 and T3, and the rest might be derived from hybridization. Hybrid lines were inferred to be resulting from the combination of these pure lines. The informative sites for discriminating the 3 taxa were detected at the nucleotide positions 122nd, 226th, 441st and 489th from upstream of the ITS sequence. For discrimination of the types of C. tangshen, including one type T0 registered in GenBank, the nucleotides at positions 135th, 489th and 500th were informative. Botanical sources of the crude drugs produced in a wide range of the southeast Gansu Prov. were C. pilosula, just those from Wenxian of Gansu Prov. were C. pilosula var. modesta. The crude drugs produced in Chongqing were derived from C. tangshen.

语种英语
所属项目编号21406004 ; 24406005 ; KHB1008
资助者JSPS KAKENHI ; Japan Health Sciences Foundation ; Expansion Program, Regional Innovation Cluster Program, Global Type (II) from MEXT, Japan ; JSPS KAKENHI ; Japan Health Sciences Foundation ; Expansion Program, Regional Innovation Cluster Program, Global Type (II) from MEXT, Japan
WOS记录号WOS:000328211400012
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66404
Collection北京大学药学院
作者单位1.Toyama Univ, Inst Nat Med, Div Pharmacognosy, Dept Med Resources, Toyama 9300194, Japan
2.Natl Inst Hlth Sci, Div Pharmacognosy Phytochem & Narcot, Setagaya Ku, Tokyo 1588501, Japan
3.Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
He, Jing Yu,Zhu, Shu,Komatsu, Katsuko,et al. Genetic polymorphism of medicinally-used Codonopsis species in an internal transcribed spacer sequence of nuclear ribosomal DNA and its application to authenticate Codonopsis Radix[J]. JOURNAL OF NATURAL MEDICINES,2014,68(1):112-124.
APA He, Jing Yu,Zhu, Shu,Komatsu, Katsuko,Goda, Yukihiro,&Cai, Shao-Qing.(2014).Genetic polymorphism of medicinally-used Codonopsis species in an internal transcribed spacer sequence of nuclear ribosomal DNA and its application to authenticate Codonopsis Radix.JOURNAL OF NATURAL MEDICINES,68(1),112-124.
MLA He, Jing Yu,et al."Genetic polymorphism of medicinally-used Codonopsis species in an internal transcribed spacer sequence of nuclear ribosomal DNA and its application to authenticate Codonopsis Radix".JOURNAL OF NATURAL MEDICINES 68.1(2014):112-124.
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