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Analytical Performance of the Abbott Architect i2000 Tacrolimus Assay in Chinese Patients After Renal Transplantation
Li, Z. Y.; Yan, C. L.; Yan, R.; Feng, Z. R.
刊名TRANSPLANTATION PROCEEDINGS
2010-12-01
DOI10.1016/j.transproceed.2010.09.155
42期:10页:4534-4537
收录类别SCI ; ISTP
文章类型Proceedings Paper
WOS标题词Science & Technology
类目[WOS]Immunology ; Surgery ; Transplantation
研究领域[WOS]Immunology ; Surgery ; Transplantation
关键词[WOS]TANDEM MASS-SPECTROMETRY ; FK506 ; CYCLOSPORINE ; FK-506 ; BLOOD
英文摘要

Background. Monitoring of tacrolimus concentrations has played a crucial role to control the efficacy versus adverse effects of treatment, because of the drug′s narrow therapeutic range, inter- and intraindividual variabilities, and drug interactions mediated by hepatic cytochrome P450. Therefore, the stability of methods to monitor tacrolimus is of major importance. This study evaluated the analytical performance of the Abbott Architect i2000 tacrolimus assay, which was recently released in China to monitor tacrolimus therapy. We compared the results using the Abbott Architect i2000 Tacrolimus assay with the traditional Abbott IMx method.

Methods. We measured concentrations of tacrolimus in 100 samples from renal transplant patients by Architect i2000 and the commonly used Abbott IMx.

Results. We observed that the total %CV of the Architect i2000 assay was <10%, both at low and high concentration levels, with a functional sensitivity of <0.5 ng/mL. The Architect i2000 assay was linear over the reportable range with recoveries ranging from 90.6% to 106.7%. In addition, this assay was not affected by high concentrations of hemoglobin, lipids, or bilirubin in the samples. The 2 assays yielded similar results. The regression equation obtained from the Passing Bablok analysis was: y = 0.8788x - 0.3485 with a Spearman correlation coefficient (r) of 0.9922. The bias plot between the Architect i2000 and Abbott IMx assay yielded an average negative bias (-1.4 ng/mL).

Conclusion. We concluded that, because of its high precision and sensitivity, low cross-reactivity, and acceptable accuracy, the Architect i2000 assay is a suitable alternative to monitor tacrolimus concentrations in renal transplant recipients.

语种英语
WOS记录号WOS:000285732200148
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66411
专题北京大学第一临床医学院
北京大学第一临床医学院_检验科
作者单位Peking Univ, Dept Clin Lab, Hosp 1, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Li, Z. Y.,Yan, C. L.,Yan, R.,et al. Analytical Performance of the Abbott Architect i2000 Tacrolimus Assay in Chinese Patients After Renal Transplantation[J]. TRANSPLANTATION PROCEEDINGS,2010,42(10):4534-4537.
APA Li, Z. Y.,Yan, C. L.,Yan, R.,&Feng, Z. R..(2010).Analytical Performance of the Abbott Architect i2000 Tacrolimus Assay in Chinese Patients After Renal Transplantation.TRANSPLANTATION PROCEEDINGS,42(10),4534-4537.
MLA Li, Z. Y.,et al."Analytical Performance of the Abbott Architect i2000 Tacrolimus Assay in Chinese Patients After Renal Transplantation".TRANSPLANTATION PROCEEDINGS 42.10(2010):4534-4537.
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