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学科主题: 临床医学
题名:
Direct chemiluminescent imaging method used to detect HbA(0), HbA(1), HbA(2) and HbF in human erythrocyte separated by polyacrylamide gel electrophoresis
作者: Shen, SH; Zhai, SD; Ouyang, J; Zhang, XH; Zhang, HY
关键词: direct chemiluminescent imaging ; polyacrylamide gel electrophoresis ; hemoglobin
刊名: ACTA CHIMICA SINICA
发表日期: 2004-07-28
卷: 62, 期:14, 页:1327-1332
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: EXPRESSION ; PROTEINS
英文摘要:

A new method of direct chemiluminescent imaging to detect proteins separated by polyacrylamide gel eletrophoresis was established. This method has been applied to the separation and detection of hemoglobins in erythrocyte of healthy voluntary people and umbilical cord blood. Serum proteins were also detected using hemoglobin as probe. Compared with the traditional Coomassie brilliant blue R250 stain method, direct chemiluminescent imaging offers a fast, convenient and inexpensive method with high sensitivity and selectivity. Furthermore, the effect of human serum albumin on the electrophoretic result of the hemoglobin was studied. The chemiluminescent imaging conditions have been optimized in the experiment.

语种: 中文
WOS记录号: WOS:000222935900012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66416
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Beijing 100083, Peoples R China
2.Beijing Jishuitan Hosp, Beijing 100875, Peoples R China
3.Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China

Recommended Citation:
Shen, SH,Zhai, SD,Ouyang, J,et al. Direct chemiluminescent imaging method used to detect HbA(0), HbA(1), HbA(2) and HbF in human erythrocyte separated by polyacrylamide gel electrophoresis[J]. ACTA CHIMICA SINICA,2004,62(14):1327-1332.
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