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学科主题临床医学
Direct chemiluminescent imaging method used to detect HbA(0), HbA(1), HbA(2) and HbF in human erythrocyte separated by polyacrylamide gel electrophoresis
Shen, SH; Zhai, SD; Ouyang, J; Zhang, XH; Zhang, HY
关键词direct chemiluminescent imaging polyacrylamide gel electrophoresis hemoglobin
刊名ACTA CHIMICA SINICA
2004-07-28
62期:14页:1327-1332
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]EXPRESSION ; PROTEINS
英文摘要

A new method of direct chemiluminescent imaging to detect proteins separated by polyacrylamide gel eletrophoresis was established. This method has been applied to the separation and detection of hemoglobins in erythrocyte of healthy voluntary people and umbilical cord blood. Serum proteins were also detected using hemoglobin as probe. Compared with the traditional Coomassie brilliant blue R250 stain method, direct chemiluminescent imaging offers a fast, convenient and inexpensive method with high sensitivity and selectivity. Furthermore, the effect of human serum albumin on the electrophoretic result of the hemoglobin was studied. The chemiluminescent imaging conditions have been optimized in the experiment.

语种中文
WOS记录号WOS:000222935900012
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66416
专题北京大学第三临床医学院
作者单位1.Peking Univ, Hosp 3, Beijing 100083, Peoples R China
2.Beijing Jishuitan Hosp, Beijing 100875, Peoples R China
3.Beijing Normal Univ, Dept Chem, Beijing 100875, Peoples R China
推荐引用方式
GB/T 7714
Shen, SH,Zhai, SD,Ouyang, J,et al. Direct chemiluminescent imaging method used to detect HbA(0), HbA(1), HbA(2) and HbF in human erythrocyte separated by polyacrylamide gel electrophoresis[J]. ACTA CHIMICA SINICA,2004,62(14):1327-1332.
APA Shen, SH,Zhai, SD,Ouyang, J,Zhang, XH,&Zhang, HY.(2004).Direct chemiluminescent imaging method used to detect HbA(0), HbA(1), HbA(2) and HbF in human erythrocyte separated by polyacrylamide gel electrophoresis.ACTA CHIMICA SINICA,62(14),1327-1332.
MLA Shen, SH,et al."Direct chemiluminescent imaging method used to detect HbA(0), HbA(1), HbA(2) and HbF in human erythrocyte separated by polyacrylamide gel electrophoresis".ACTA CHIMICA SINICA 62.14(2004):1327-1332.
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