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学科主题: 临床医学
题名:
Drug-resistant genes carried by Acinetobacter baumanii isolated from patients with lower respiratory tract infection
作者: Dai Ning1; Li De-zhi1; Chen Ji-chao1; Chen Yu-sheng4; Geng Rong3; Hu Ying-hui3; Yang Jing-ping5; Du Juan6; Hu Cheng-ping7; Zhang Wei8; Li Jia-shu9; Yu Qin10; Wan Huan-ying11; Mu Lan12; Zhong Xiao-ning13; We Li-ping14; Ma Jian-jun15; Wang Qiu-yue16; Hu Ke17; Tian Gui-zhen18; Cai Shao-xi19; Wang Rui-qin20; He Bei21; Wang Si-qin22; Wang Zhan-wei2; Zhao Su-rui2; Gao Zhan-cheng1
关键词: Acinetobacter baumanii ; lower respiratory tract infections ; beta-lactamase ; drug-resistant gene
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2010-09-20
DOI: 10.3760/cma.j.issn.0366-6999.2010.18.015
卷: 123, 期:18, 页:2571-2575
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: BETA-LACTAMASES ; OUTBREAK ; STRAINS ; ENZYME
英文摘要:

Background Acinetobacter baumanii (A. baumanii) remains an important microbial pathogen resulting in nosocomial acquired infections with significant morbidity and mortality. The mechanism by which nosocomial bacteria, like A. baumanii, attain multidrug resistance to antibiotics is of considerable interest. The aim in this study was to investigate the spread status of antibiotic resistance genes, such as multiple beta-lactamase genes and aminoglycoside-modifying enzyme genes, from A. baumanii strains isolated from patients with lower respiratory tract infections (LRTIs).

Methods Two thousand six hundred and ninety-eight sputum or the bronchoalveolar lavage samples from inpatients with LRTIs were collected in 21 hospitals in the mainland of China from November 2007 to February 2009. All samples were routinely inoculated. The isolated bacterial strains and their susceptibility were analyzed via VITEK-2 expert system. Several kinds of antibiotic resistant genes were further differentiated via polymerase chain reaction and sequencing methods.

Results Totally, 39 A. baumanii strains were isolated from 2698 sputum or bronchoalveolar lavage samples. There was not only a high resistant rate of the isolated A. baumanii strains to ampicillin and first- and second-generation cephalosporins (94.87%, 100% and 97.44%, respectively), but also to the third-generation cephalosporins (ceftriaxone at 92.31%, ceftazidine at 51.28%) and imipenem (43.59%) as well. The lowest antibiotic resistance rate of 20.51% was found to amikacin. The OXA-23 gene was identified in 17 strains of A. baumanii, and the AmpC gene in 23 strains. The TEM-1 gene was carried in 15 strains. PER-1 and SHV-2 genes were detected in two different strains. Aminoglycoside-modifying enzyme gene aac-3-la was found in 23 strains, and the aac-6′-lb gene in 19 strains. aac-3-la and aac-6′-lb genes hibernated in three A. baumanii strains that showed no drug-resistant phenotype.

Conclusions A. baumanii can carry multiple drug-resistant genes at the same time and result in multi-drug resistance. Aminoglycoside-modifying enzyme genes could be hibernating in aminoglycoside sensitive strains without expressing their phenotype. Chin Med J 2010;123(18):2571-2575

语种: 英语
所属项目编号: 2006AA02Z4A9
项目资助者: National Hi-Tech Project
WOS记录号: WOS:000282806200015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66424
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Resp & Crit Care Med, Beijing 100044, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Microbiol, Beijing 100044, Peoples R China
3.Capital Med Univ, Beijing Childrens Hosp, Dept Resp Med, Beijing 100045, Peoples R China
4.Fujian Prov Hosp, Dept Resp Med, Fuzhou 350001, Fujian, Peoples R China
5.Aerosp Cent Hosp, Dept Resp Med, Beijing 100049, Peoples R China
6.Lanzhou Pulm Hosp, Dept Resp Med, Lanzhou 730046, Gansu, Peoples R China
7.Inner Mongolia Med Coll, Affiliated Hosp 3, Dept Resp Med, Baotou 014010, Inner Mongolia, Peoples R China
8.Guiyang Med Coll, Affiliated Hosp, Dept Resp Med, Guiyang 550004, Guizhou, Peoples R China
9.Cent S Univ, Xiangya Hosp, Dept Resp Med, Changsha 410008, Hunan, Peoples R China
10.Nanchang Univ, Affiliated Hosp 1, Dept Resp Med, Nanchang 330006, Jiangxi, Peoples R China
11.Xuzhou Med Coll, Lianyungang Peoples Hosp 1, Dept Resp Med, Lianyungang 222002, Jiangsu, Peoples R China
12.Lanzhou Univ, Hosp 1, Dept Resp Med, Lanzhou 730000, Gansu, Peoples R China
13.Shanghai Jiao Tong Univ, Ruijin Hosp, Dept Resp Med, Shanghai 200025, Peoples R China
14.Guangxi Med Univ, Affiliated Hosp 1, Dept Resp Med, Nanning 530021, Guangxi, Peoples R China
15.Guangzhou Med Univ, Affiliated Hosp 3, Dept Resp Med, Guangzhou 510150, Guangdong, Peoples R China
16.China Med Univ, Affiliated Hosp 1, Inst Resp Dis, Shenyang 110001, Liaoning, Peoples R China
17.Wuhan Univ, Renmin Hosp, Dept Resp Med, Wuhan 430060, Hubei, Peoples R China
18.Mil Gen Hosp Beijing, Clin Sect 263, Dept Resp Med, Beijing 101149, Peoples R China
19.So Med Univ, Nanfang Hosp, Dept Resp Med, Guangzhou 510515, Guangdong, Peoples R China
20.Tsinghua Univ, Affiliated Hosp 1, Dept Resp Med, Beijing 100016, Peoples R China
21.Peking Univ, Hosp 3, Dept Resp Med, Beijing 100191, Peoples R China
22.Henan Prov People Hosp, Dept Resp Med, Zhengzhou 450003, Henan, Peoples R China

Recommended Citation:
Dai Ning,Li De-zhi,Chen Ji-chao,et al. Drug-resistant genes carried by Acinetobacter baumanii isolated from patients with lower respiratory tract infection[J]. CHINESE MEDICAL JOURNAL,2010,123(18):2571-2575.
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