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学科主题: 临床医学
题名:
Promoter hypermethylation of PTPL1, PTPN6, DAPK, p16 and 5-azacitidine inhibits growth in DLBCL
作者: Wang, Wenming1; Wang, Jing1; Li, Zhengqian2; Zhu, Mingxia1; Zhang, Zhe3; Wang, Yanfang1; Jing, Hongmei1
关键词: protein tyrosine phosphatase 1 ; PTPN6 ; DAPK ; p16 ; diffuse large B cell lymphoma ; methylation ; 5-azacitidine
刊名: ONCOLOGY REPORTS
发表日期: 2016
DOI: 10.3892/or.2015.4347
卷: 35, 期:1, 页:139-146
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: B-CELL LYMPHOMA ; EPIGENETIC THERAPY ; GENE-EXPRESSION ; DNA METHYLATION ; CANCER ; PROLIFERATION ; APOPTOSIS ; DNMT3B ; BAX
英文摘要:

Aberrant hypermethylation of CpG islands of tumor suppressor is one of the mechanisms for epigenetic loss of gene function. In the present study, the methylation status of the promoter regions of protein tyrosine phosphatase (PTPN) 6, DAPK, and p16 were studied using methylation-specific polymerase chain reaction (MSP) in 26 diffuse large B cell lymphoma (DLBCL) lymphomas. In OCI-LY1 cell line, gene methylation status, expression of PTPL1 and its reactivation by DNA demethylation was determined by PCR and on the protein level by western blotting. ELISA-like reaction was used to detect global DNA methylation measurement. Induction of apoptosis by 5-azacitidine was analyzed by Annexin V/PI staining and flow cytometry. Our results show that hypermethylation of the PTPN6 gene promoter region was found in 15.4% (4/26), the DAPK gene promoter region in 30.8% (8/26), the p16 gene promoter region in 7.7% (2/26). Notably, we identified that PTPL1 was hypermethylated and transcriptionally silenced in OCI-LY1 cell line. The expression of PTPL1 was re-inducible by 5-azacytidine. 5-azacytidine also inhibits the proliferation and decreases the global methylation level of the OCI-LY1 cell line. We can conclude from our study that a higher prevalence of methylation of PTPL1, PTPN6, DAPK and p16 occur in DLBCL. Our data also highlights 5-azacytidine as a potential therapeutic candidate for DLBCL. Further studies are required to substantiate the role of methylation of PTPL1, PTPN6, DAPK and p16 as a marker in diffuse large B cell lymphoma.

语种: 英语
WOS记录号: WOS:000366072500016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66432
Appears in Collections:北京大学第三临床医学院_血液内科_期刊论文

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作者单位: 1.Peking Univ, Dept Hematol, Hosp 3, Beijing 100191, Peoples R China
2.Peking Univ, Dept Anesthesiol, Hosp 3, Beijing 100191, Peoples R China
3.Peking Univ, Dept Urol, Hosp 3, Beijing 100191, Peoples R China

Recommended Citation:
Wang, Wenming,Wang, Jing,Li, Zhengqian,et al. Promoter hypermethylation of PTPL1, PTPN6, DAPK, p16 and 5-azacitidine inhibits growth in DLBCL[J]. ONCOLOGY REPORTS,2016,35(1):139-146.
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