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学科主题: 临床医学
题名:
Icariin stimulates the proliferation of rat bone mesenchymal stem cells via ERK and p38 MAPK signaling
作者: Qin, Shuyan1; Zhou, Wei2; Liu, Shaoying3; Chen, Puxiang4; Wu, Hongjin1
关键词: Icariin ; bone mesenchymal stem cells ; ERK ; p38 MAPK
刊名: INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
发表日期: 2015
卷: 8, 期:5, 页:7125-7133
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: IN-VITRO ; STROMAL CELLS ; OSTEOGENESIS IMPERFECTA ; DIFFERENTIATION ; EXPRESSION ; TRANSPLANTATION ; ACTIVATION ; INHIBITOR ; CHILDREN ; SURVIVAL
英文摘要:

Bone mesenchymal stem cells (BMSCs) are able to differentiate into multi types of lineages, so they have been widely applied in the stem cell transplantation. The BMSCs are usually needed to be expanded before transplantation due to their limited content in bone marrow. It has recently been reported that Icariin (ICA), a major constituent of flavonoids from the Chinese medical herb Epimedium brevicornum Maxim, promotes the proliferation of various types of differentiated cells. However, whether ICA can enhance BMSCs proliferation and the possible underlying mechanisms are still unknown. After being isolated and purified from rat bone marrow, cultured BMSCs are stimulated with different concentrations of ICA. The cytotoxicity of ICA is evaluated by the Cell Counting Kit-8 (CCK-8) assay method and the ICA optimal concentration for BMSCs proliferation is determined at 320 mu g/L. Our work reveals that ICA induces an obvious phosphorylation of ERK and p38 kinases in BMSCs, no matter serum exists or not. Inhibition of ERK or p38 MAPK signaling by their specific inhibitors PD98059 or SP600125, respectively, not only prevents the activation of these kinases, but also attenuates cell proliferation induced by ICA. Furthermore, the downstream transcription factors of MAPK pathway, Elk1, Stat3, c-Myc and Fos, are also monitored by RT-PCR, and our results show that among them, Elk1 and c-Myc are significantly upregulated after ICA treatment. Taken together, our results demonstrate that ICA promotes the proliferation of rat BMSCs through activating ERK and p38 MAPK signaling which further leads to upregulation of their downstream transcription factors Elk1 and c-Myc. Our work provides a novel effective way to expand the content of BMSCs in vitro, which casts light on clinical applications of stem cell transplantation in the future.

语种: 英语
所属项目编号: JZZ-312
项目资助者: Beijing Key Disciplines Project of Traditional Chinese Medicine
WOS记录号: WOS:000359293200050
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66445
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Haidian Sect, Beijing Haidian Hosp, Beijing, Peoples R China
2.Hubei Univ Med, Taihe Hosp, Dept Orthoped Microsurg, Shiyan 442000, Peoples R China
3.Beijing Hosp Integrated Tradit Chinese & Western, Beijing, Peoples R China
4.Cent S Univ, Xiangya Hosp 2, Dept Gynecol & Obstet, Changsha 410011, Hunan, Peoples R China

Recommended Citation:
Qin, Shuyan,Zhou, Wei,Liu, Shaoying,et al. Icariin stimulates the proliferation of rat bone mesenchymal stem cells via ERK and p38 MAPK signaling[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,2015,8(5):7125-7133.
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