学科主题基础医学
DA neurons derived from hES cells that express HLA-G1 are capable of immunosuppression
Zhu, Yajing3,4; Zhao, Hongxi5; Wang, Li1,2; Zhao, Sanjun6; Jiang, Feng5; Li, Lingsong1,2; Yao, Yuanqing3
关键词Human embryonic stem cell Human leukocyte antigen G Differentiation Immune rejection
刊名BRAIN RESEARCH
2012-02-09
DOI10.1016/j.brainres.2011.12.033
1437期:0页:134-142
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
资助者Chinese National 973 Projects ; Chinese National 973 Projects
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]EMBRYONIC STEM-CELLS ; DOPAMINERGIC-NEURONS ; ES CELLS ; DIFFERENTIATION ; BLASTOCYSTS ; INDUCTION ; COCULTURE ; ANTIGEN ; SYSTEM
英文摘要

Human embryonic stem (hES) cells have the capacity for self-renewal and exhibit multipotentiality. hES cells have promise for serving as an unlimited source of ideal seed cells for cell transplantation. However, the rejection that occurs between the transplant recipient and the transplanted cell poses a major challenge for therapeutic transplantation. This study was designed to devise methods to enhance immune tolerance in cell therapy. We established an hES cell line that could stably express human leukocyte antigen-G1 (HLA-G1). The established HLA-G1-H1 hES cells still retained all the characteristics of normal human embryonic stem cells. By using the SDIA method, we induced dopaminergic (DA) neurons by coculturing HLA-G1-H1 hES cells with the mouse stromal cell line PA6. Tyrosine hydroxylase (TH)+neurons were detected on the 10th day of differentiation, and 70% of the HLA-G1-H1 hES cells were TH+mature DA neurons because the differentiation time was only 3 weeks. Cells that had been differentiating for different periods of time still expressed HLA-G1, and these differentiated DA neurons released dopamine and other catecholamines in response to K+ depolarization as measured by HPLC. After careful study, we found that HLA-G1-H1 hES cells are capable of inhibiting the proliferation of mixed T-lymphocytes. DA neurons derived from HLA-G1-H1 hES attenuated the release of proinflammatory cytokines IL-1 beta and IFN-gamma from lipopolysaccharide (LPS)-stimulated BV2 microglia. The efficiency of inhibition was significant and dose-dependent. This method might be used to treat Parkinson′s patients via cell transplantation. (C) 2011 Elsevier B.V. All rights reserved.

语种英语
所属项目编号2007CB948102
资助者Chinese National 973 Projects ; Chinese National 973 Projects
WOS记录号WOS:000300965700013
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66456
专题北京大学基础医学院_北京大学干细胞研究中心
作者单位1.Peking Univ, Stem Cell Res Ctr, Beijing 100191, Peoples R China
2.China Natl Ctr Int Res, Beijing 100191, Peoples R China
3.Nankai Univ, Sch Med, Tianjin 30071, Peoples R China
4.Peoples Liberat Army, Dept Obstet & Gynecol, Gen Hosp, Beijing 100853, Peoples R China
5.Fourth Mil Med Univ, Dept Obstet & Gynecol, Tangdu Hosp, Xian 710038, Peoples R China
6.Peoples Liberat Army, Dept Anesthesiol, Gen Hosp, Beijing 100853, Peoples R China
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GB/T 7714
Zhu, Yajing,Zhao, Hongxi,Wang, Li,et al. DA neurons derived from hES cells that express HLA-G1 are capable of immunosuppression[J]. BRAIN RESEARCH,2012,1437(0):134-142.
APA Zhu, Yajing.,Zhao, Hongxi.,Wang, Li.,Zhao, Sanjun.,Jiang, Feng.,...&Yao, Yuanqing.(2012).DA neurons derived from hES cells that express HLA-G1 are capable of immunosuppression.BRAIN RESEARCH,1437(0),134-142.
MLA Zhu, Yajing,et al."DA neurons derived from hES cells that express HLA-G1 are capable of immunosuppression".BRAIN RESEARCH 1437.0(2012):134-142.
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