IR@PKUHSC  > 北京大学基础医学院
学科主题基础医学
GATA family members as inducers for cellular reprogramming to pluripotency
Shu, Jian1; Zhang, Ke1,2; Zhang, Minjie3,4; Yao, Anzhi1; Shao, Sida1; Du, Fengxia3; Yang, Caiyun3; Chen, Wenhan1; Wu, Chen1; Yang, Weifeng5; Sun, Yingli3; Deng, Hongkui1,6,7
关键词GATA transcription factors lineage specifier iPSCs
刊名CELL RESEARCH
2015-02-01
DOI10.1038/cr.2015.6
25期:2页:169-180
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]LINEAGE SPECIFIERS ; STEM-CELLS ; TRANSCRIPTION FACTORS ; HUMAN FIBROBLASTS ; DEFINED FACTORS ; RNA-SEQ ; INTERACTION NETWORK ; GENE-EXPRESSION ; SOMATIC-CELLS ; MOUSE
英文摘要

Members of the GATA protein family play important roles in lineage specification and transdifferentiation. Previous reports show that some members of the GATA protein family can also induce pluripotency in somatic cells by substituting for Oct4, a key pluripotency-associated factor. However, the mechanism linking lineage-specifying cues and the activation of pluripotency remains elusive. Here, we report that all GATA family members can substitute for Oct4 to induce pluripotency. We found that all members of the GATA family could inhibit the overrepresented ectodermal-lineage genes, which is consistent with previous reports indicating that a balance of different lineage-specifying forces is important for the restoration of pluripotency. A conserved zinc-finger DNA-binding domain in the C-terminus is critical for the GATA family to induce pluripotency. Using RNA-seq and ChIP-seq, we determined that the pluripotency-related gene Sall4 is a direct target of GATA family members during reprogramming and serves as a bridge linking the lineage-specifying GATA family to the pluripotency circuit. Thus, the GATA family is the first protein family of which all members can function as inducers of the reprogramming process and can substitute for Oct4. Our results suggest that the role of GATA family in reprogramming has been underestimated and that the GATA family may serve as an important mediator of cell fate conversion.

语种英语
WOS记录号WOS:000349334100006
项目编号2012CB966401 ; 91319305 ; 91019024 ; 31100558 ; 2013ZX10001003 ; 2013DFG30680 ; XDA01040407
资助机构National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China ; National Science and Technology Major Project ; Ministry of Science and Technology of China ; Ministry of Education of China (111 Project) ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66458
专题北京大学基础医学院
北京大学第三临床医学院_耳鼻喉科
作者单位1.Beijing Vitalstar Biotechnol Co Ltd, Beijing 100012, Peoples R China
2.Peking Univ, Peking Tsinghua Ctr Life Sci, Coll Life Sci, MOE Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
4.Chinese Acad Sci, Beijing Inst Genom, Key Lab Genom & Precis Med, Beijing 100101, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Peking Univ, Shenzhen Grad Sch, Key Lab Chem Genom, Shenzhen Stem Cell Engn Lab, Shenzhen 518055, Guangdong, Peoples R China
7.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Stem Cell Res Ctr,Dept Cell Biol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Shu, Jian,Zhang, Ke,Zhang, Minjie,et al. GATA family members as inducers for cellular reprogramming to pluripotency[J]. CELL RESEARCH,2015,25(2):169-180.
APA Shu, Jian.,Zhang, Ke.,Zhang, Minjie.,Yao, Anzhi.,Shao, Sida.,...&Deng, Hongkui.(2015).GATA family members as inducers for cellular reprogramming to pluripotency.CELL RESEARCH,25(2),169-180.
MLA Shu, Jian,et al."GATA family members as inducers for cellular reprogramming to pluripotency".CELL RESEARCH 25.2(2015):169-180.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
GATA family members (6244KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Shu, Jian]的文章
[Zhang, Ke]的文章
[Zhang, Minjie]的文章
百度学术
百度学术中相似的文章
[Shu, Jian]的文章
[Zhang, Ke]的文章
[Zhang, Minjie]的文章
必应学术
必应学术中相似的文章
[Shu, Jian]的文章
[Zhang, Ke]的文章
[Zhang, Minjie]的文章
相关权益政策
暂无数据
收藏/分享
文件名: GATA family members as inducers for cellular reprogramming to pluripotency.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。