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学科主题: 基础医学
题名:
GATA family members as inducers for cellular reprogramming to pluripotency
作者: Shu, Jian1; Zhang, Ke1,2; Zhang, Minjie3,4; Yao, Anzhi1; Shao, Sida1; Du, Fengxia3; Yang, Caiyun3; Chen, Wenhan1; Wu, Chen1; Yang, Weifeng5; Sun, Yingli3; Deng, Hongkui1,6,7
关键词: GATA transcription factors ; lineage specifier ; iPSCs
刊名: CELL RESEARCH
发表日期: 2015-02-01
DOI: 10.1038/cr.2015.6
卷: 25, 期:2, 页:169-180
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: LINEAGE SPECIFIERS ; STEM-CELLS ; TRANSCRIPTION FACTORS ; HUMAN FIBROBLASTS ; DEFINED FACTORS ; RNA-SEQ ; INTERACTION NETWORK ; GENE-EXPRESSION ; SOMATIC-CELLS ; MOUSE
英文摘要:

Members of the GATA protein family play important roles in lineage specification and transdifferentiation. Previous reports show that some members of the GATA protein family can also induce pluripotency in somatic cells by substituting for Oct4, a key pluripotency-associated factor. However, the mechanism linking lineage-specifying cues and the activation of pluripotency remains elusive. Here, we report that all GATA family members can substitute for Oct4 to induce pluripotency. We found that all members of the GATA family could inhibit the overrepresented ectodermal-lineage genes, which is consistent with previous reports indicating that a balance of different lineage-specifying forces is important for the restoration of pluripotency. A conserved zinc-finger DNA-binding domain in the C-terminus is critical for the GATA family to induce pluripotency. Using RNA-seq and ChIP-seq, we determined that the pluripotency-related gene Sall4 is a direct target of GATA family members during reprogramming and serves as a bridge linking the lineage-specifying GATA family to the pluripotency circuit. Thus, the GATA family is the first protein family of which all members can function as inducers of the reprogramming process and can substitute for Oct4. Our results suggest that the role of GATA family in reprogramming has been underestimated and that the GATA family may serve as an important mediator of cell fate conversion.

语种: 英语
所属项目编号: 2012CB966401 ; 91319305 ; 91019024 ; 31100558 ; 2013ZX10001003 ; 2013DFG30680 ; XDA01040407
项目资助者: National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China ; National Science and Technology Major Project ; Ministry of Science and Technology of China ; Ministry of Education of China (111 Project) ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Hundred Talents Program of the Chinese Academy of Sciences
WOS记录号: WOS:000349334100006
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66458
Appears in Collections:基础医学院_期刊论文

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作者单位: 1.Beijing Vitalstar Biotechnol Co Ltd, Beijing 100012, Peoples R China
2.Peking Univ, Peking Tsinghua Ctr Life Sci, Coll Life Sci, MOE Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
4.Chinese Acad Sci, Beijing Inst Genom, Key Lab Genom & Precis Med, Beijing 100101, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
6.Peking Univ, Shenzhen Grad Sch, Key Lab Chem Genom, Shenzhen Stem Cell Engn Lab, Shenzhen 518055, Guangdong, Peoples R China
7.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Stem Cell Res Ctr,Dept Cell Biol, Beijing 100191, Peoples R China

Recommended Citation:
Shu, Jian,Zhang, Ke,Zhang, Minjie,et al. GATA family members as inducers for cellular reprogramming to pluripotency[J]. CELL RESEARCH,2015,25(2):169-180.
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