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学科主题口腔医学
Up-regulation of estrogen receptor-beta expression during osteogenic differentiation of human periodontal ligament cells
Tang, X.1,2; Meng, H.1; Han, J.1; Zhang, L.1; Hou, J.1; Zhang, F.1
关键词estrogen receptors human periodontal ligament cells osteogenic differentiation stem cells
刊名JOURNAL OF PERIODONTAL RESEARCH
2008-06-01
DOI10.1111/j.1600-0765.2007.01031.x
43期:3页:311-321
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]BONE-MINERAL DENSITY ; POLYMERASE CHAIN-REACTION ; NODULES FORMED INVITRO ; MESSENGER-RNA ; ER-BETA ; POSTMENOPAUSAL WOMEN ; GENE POLYMORPHISMS ; INTERNAL CONTROL ; ALPHA ; TRANSCRIPTION
英文摘要

Background and Objective: Estrogen has been shown to up-regulate the expression of osteoblastic phenotypes of human periodontal ligament cells via binding to estrogen receptors and may also help periodontal tissue regeneration. However, which subtype of estrogen receptor (alpha or beta) is predominately expressed in human periodontal ligament cells, and how estrogen receptor expression is regulated during the osteogenic differentiation of human periodontal ligament cells, is still unclear. This study aimed to explore the expression and regulation of estrogen receptor subtypes in human periodontal ligament cells and during their osteogenic differentiation.

Material and Methods: Human periodontal ligament cells derived from 10 individual age-matched donors (five male and five female donors) were cultured. Human periodontal ligament cells under osteogenic induction (group M) and the corresponding controls (group C) were harvested on days 7, 14 and 21 for estrogen receptor detection.

Results: Both estrogen receptor-alpha and estrogen receptor-beta mRNAs were expressed in human periodontal ligament cells from all of the 10 donors. Protein only of estrogen receptor-beta (not of estrogen receptor-alpha) was detected and was shown to be located in the nuclei of human periodontal ligament cells. The expression levels of estrogen receptor-beta mRNA and protein from both male and female donors in group M were significantly higher compared with group C during the 21-d study period. In comparison, the expression level of estrogen receptor-alpha mRNA of the donors was not significantly different from that of the controls during osteogenic differentiation and no estrogen receptor-alpha protein was detected.

Conclusion: The results suggest that estrogen receptor-beta may be the predominant subtype expressed in human periodontal ligament cells and may actively participate in the osteogenic differentiation process of human periodontal ligament cells, both in male and in female subjects.

语种英语
WOS记录号WOS:000255464700008
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66461
Collection北京大学口腔医学院_牙周科
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100081, Haidian, Peoples R China
2.China Med Univ, Sch Stomatol, Dept Periodontol, Shenyang, Peoples R China
Recommended Citation
GB/T 7714
Tang, X.,Meng, H.,Han, J.,et al. Up-regulation of estrogen receptor-beta expression during osteogenic differentiation of human periodontal ligament cells[J]. JOURNAL OF PERIODONTAL RESEARCH,2008,43(3):311-321.
APA Tang, X.,Meng, H.,Han, J.,Zhang, L.,Hou, J.,&Zhang, F..(2008).Up-regulation of estrogen receptor-beta expression during osteogenic differentiation of human periodontal ligament cells.JOURNAL OF PERIODONTAL RESEARCH,43(3),311-321.
MLA Tang, X.,et al."Up-regulation of estrogen receptor-beta expression during osteogenic differentiation of human periodontal ligament cells".JOURNAL OF PERIODONTAL RESEARCH 43.3(2008):311-321.
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