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学科主题: 临床医学
题名:
Sorafenib Induces Growth Inhibition and Apoptosis of Human Chondrosarcoma Cells by Blocking the RAF/ERK/MEK Pathway
作者: Lu, Xinchang; Tang, Xiaodong; Guo, Wei; Ren, Tingting; Zhao, Hui
关键词: chondrosarcoma ; sorafenib ; MAPK ; apoptosis
刊名: JOURNAL OF SURGICAL ONCOLOGY
发表日期: 2010-12-01
DOI: 10.1002/jso.21661
卷: 102, 期:7, 页:821-826
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Surgery
研究领域[WOS]: Oncology ; Surgery
关键词[WOS]: DOWN-REGULATION ; CANCER ; P38 ; BAY-43-9006 ; CARCINOMA ; MIGRATION ; SARCOMA ; ERK
英文摘要:

Background: Chondrosarcoma represents the second most common primary malignant bone tumor causing significant morbidity due to local recurrence and limited treatment options. Conventional cytotoxic chemotherapy has been proven to be largely ineffective to this sarcoma. Here we report that sorafenib is effective in growth inhibition of chondrosarcoma cell lines in vitro.

Methods: Chondrosarcoma cell lines (SW 1353 and CRL7891) were treated with sorafenib. Flow cytometry, DAPI assay, and Western blotting were employed to determine the effects of sorafenib in inhibitory proliferation and induce apoptosis in chondrosarcoma cells in vitro.

Results: The results showed that sorafenib effectively inhibited cell growth and induced apoptosis in chondrosarcoma cells, which was concurrent with inhibition of the expression of phospho-MEK and phospho-ERK. Further more the expression levels of cyclin D1, Rb and anti-apoptotic proteins Bcl-xl and Mcl-1 significantly reduced, but no changes in Bcl-2 and Bax. We although detected the expression of Akt, JNK, p38 and their respective phosphoprotein, but did not found meaningful changes.

Conclusions: Our findings demonstrate that sorafenib inhibited the Ras/Raf/MAPK pathway in a time-and dose-dependent fashion in chondrosarcoma cell lines SW 1353 and CRL7891 and suggest that sorafenib may be a new therapeutic option for patients with chondrosarcorna. J. Surg. Oncol. 2010;102:821-826. (C) 2010 Wiley-Liss, Inc.

语种: 英语
所属项目编号: 30700838 ; 20070001775
项目资助者: National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China
WOS记录号: WOS:000285281300022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66468
Appears in Collections:北京大学第二临床医学院_骨肿瘤科_期刊论文

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作者单位: Peking Univ, Musculoskeletal Tumor Ctr, Peoples Hosp, Beijing 100044, Peoples R China

Recommended Citation:
Lu, Xinchang,Tang, Xiaodong,Guo, Wei,et al. Sorafenib Induces Growth Inhibition and Apoptosis of Human Chondrosarcoma Cells by Blocking the RAF/ERK/MEK Pathway[J]. JOURNAL OF SURGICAL ONCOLOGY,2010,102(7):821-826.
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