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学科主题临床医学
17 beta-Estradiol Attenuates Neural Cell Apoptosis Through Inhibition of JNK Phosphorylation in SCI Rats and Excitotoxicity Induced by Glutamate In Vitro
Rong, Wei; Wang, Jun; Liu, Xiaoguang; Jiang, Liang; Wei, Feng; Zhou, Hua; Han, Xiaoguang; Liu, Zhongjun
关键词apoptosis JNK spinal cord injury 17 beta-estradiol
刊名INTERNATIONAL JOURNAL OF NEUROSCIENCE
2012-07-01
DOI10.3109/00207454.2012.668726
122期:7页:381-387
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
资助者Nature Science Foundation of China ; Nature Science Foundation of China
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]SPINAL-CORD-INJURY ; PROTEIN-KINASE PATHWAY ; OXIDATIVE STRESS ; ACTIVATION ; ESTROGEN ; NEUROPROTECTION ; TOXICITY ; OLIGODENDROCYTES ; ANTAGONIST ; MECHANISM
英文摘要

We investigated whether 17 beta-estradiol (E2) treatment could prevent the apoptosis of neural cells after spinal cord injury (SCI) and cultured cortical cells through inhibition of JNK (c-Jun N-terminal kinase) phosphorylation. SCI-induced rats were randomly divided into three groups: control, E2-treated, and sham-treated. Five rats from each group were sacrificed at 2, 4, 6, 12, or 24 h postinjury. Apoptotic neural cells were assessed using the TUNEL method. JNK phosphorylation was detected with immunohistochemistry. Cultured cortical cells were pretreated with E2 and the specific JNK inhibitor SP600125 and then treated with glutamate-induced cytotoxicity in vitro. Neuron viability was determined with an methyl thiazolyl tetrazolium (MTT) assay, morphology of apoptotic cells was observed with 4′,6-diamidino-2-phenylindole (DAPI) staining, and JNK phosphorylation was detected using Western blot analysis. Treatment with E2 reduced neuron apoptosis and inhibited JNK phosphorylation. Moreover, the number of apoptotic cells was correlated with JNK phosphorylation 24 h after the rats suffered the SCI. Pretreatment with E2 significantly maintained neural cell viability, attenuated apoptosis, and inhibited JNK phosphorylation induced by glutamate in vitro. These neuroprotective effects of E2 on neural cells were blocked by the co-administration of SP600125. Our results suggest that neuroprotection from E2 is partially mediated by the inhibition of JNK phosphorylation.

语种英语
所属项目编号58441-06
资助者Nature Science Foundation of China ; Nature Science Foundation of China
WOS记录号WOS:000305011800009
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66473
Collection北京大学第三临床医学院_骨科
作者单位Peking Univ, Hosp 3, Dept Orthopaed, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Rong, Wei,Wang, Jun,Liu, Xiaoguang,et al. 17 beta-Estradiol Attenuates Neural Cell Apoptosis Through Inhibition of JNK Phosphorylation in SCI Rats and Excitotoxicity Induced by Glutamate In Vitro[J]. INTERNATIONAL JOURNAL OF NEUROSCIENCE,2012,122(7):381-387.
APA Rong, Wei.,Wang, Jun.,Liu, Xiaoguang.,Jiang, Liang.,Wei, Feng.,...&Liu, Zhongjun.(2012).17 beta-Estradiol Attenuates Neural Cell Apoptosis Through Inhibition of JNK Phosphorylation in SCI Rats and Excitotoxicity Induced by Glutamate In Vitro.INTERNATIONAL JOURNAL OF NEUROSCIENCE,122(7),381-387.
MLA Rong, Wei,et al."17 beta-Estradiol Attenuates Neural Cell Apoptosis Through Inhibition of JNK Phosphorylation in SCI Rats and Excitotoxicity Induced by Glutamate In Vitro".INTERNATIONAL JOURNAL OF NEUROSCIENCE 122.7(2012):381-387.
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