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学科主题: 临床医学
题名:
17 beta-Estradiol Attenuates Neural Cell Apoptosis Through Inhibition of JNK Phosphorylation in SCI Rats and Excitotoxicity Induced by Glutamate In Vitro
作者: Rong, Wei; Wang, Jun; Liu, Xiaoguang; Jiang, Liang; Wei, Feng; Zhou, Hua; Han, Xiaoguang; Liu, Zhongjun
关键词: apoptosis ; JNK ; spinal cord injury ; 17 beta-estradiol
刊名: INTERNATIONAL JOURNAL OF NEUROSCIENCE
发表日期: 2012-07-01
DOI: 10.3109/00207454.2012.668726
卷: 122, 期:7, 页:381-387
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: SPINAL-CORD-INJURY ; PROTEIN-KINASE PATHWAY ; OXIDATIVE STRESS ; ACTIVATION ; ESTROGEN ; NEUROPROTECTION ; TOXICITY ; OLIGODENDROCYTES ; ANTAGONIST ; MECHANISM
英文摘要:

We investigated whether 17 beta-estradiol (E2) treatment could prevent the apoptosis of neural cells after spinal cord injury (SCI) and cultured cortical cells through inhibition of JNK (c-Jun N-terminal kinase) phosphorylation. SCI-induced rats were randomly divided into three groups: control, E2-treated, and sham-treated. Five rats from each group were sacrificed at 2, 4, 6, 12, or 24 h postinjury. Apoptotic neural cells were assessed using the TUNEL method. JNK phosphorylation was detected with immunohistochemistry. Cultured cortical cells were pretreated with E2 and the specific JNK inhibitor SP600125 and then treated with glutamate-induced cytotoxicity in vitro. Neuron viability was determined with an methyl thiazolyl tetrazolium (MTT) assay, morphology of apoptotic cells was observed with 4′,6-diamidino-2-phenylindole (DAPI) staining, and JNK phosphorylation was detected using Western blot analysis. Treatment with E2 reduced neuron apoptosis and inhibited JNK phosphorylation. Moreover, the number of apoptotic cells was correlated with JNK phosphorylation 24 h after the rats suffered the SCI. Pretreatment with E2 significantly maintained neural cell viability, attenuated apoptosis, and inhibited JNK phosphorylation induced by glutamate in vitro. These neuroprotective effects of E2 on neural cells were blocked by the co-administration of SP600125. Our results suggest that neuroprotection from E2 is partially mediated by the inhibition of JNK phosphorylation.

语种: 英语
所属项目编号: 58441-06
项目资助者: Nature Science Foundation of China
WOS记录号: WOS:000305011800009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66473
Appears in Collections:北京大学第三临床医学院_骨科_期刊论文

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作者单位: Peking Univ, Hosp 3, Dept Orthopaed, Beijing 100191, Peoples R China

Recommended Citation:
Rong, Wei,Wang, Jun,Liu, Xiaoguang,et al. 17 beta-Estradiol Attenuates Neural Cell Apoptosis Through Inhibition of JNK Phosphorylation in SCI Rats and Excitotoxicity Induced by Glutamate In Vitro[J]. INTERNATIONAL JOURNAL OF NEUROSCIENCE,2012,122(7):381-387.
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