IR@PKUHSC  > 北京大学第三临床医学院  > 生殖医学中心
学科主题临床医学
Ghrelin promotes the differentiation of human embryonic stem cells in infarcted cardiac microenvironment
Gao, Meijuan2,3; Yang, Jin2,3; Liu, Guoqiang2,3; Wei, Rui2,3; Zhang, Lin2,3; Wang, Haining2,3; Wang, Guang2,3; Gao, Hongwei2,3; Chen, Guian1,3; Hong, Tianpei2,3
关键词Ghrelin Embryonic stem cells Differentiation Myocardial infarction
刊名PEPTIDES
2012-04-01
DOI10.1016/j.peptides.2012.02.006
34期:2页:373-379
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]MYOCARDIAL-INFARCTION ; ENDOTHELIAL-CELLS ; HEART ; PEPTIDE ; SOX9 ; RATS ; CARDIOMYOCYTES ; EXPRESSION ; SECRETION
英文摘要

Ghrelin is broadly expressed in myocardial tissues, where it exerts different functions. It also has been found to have a wide variety of biological functions on cell differentiation and tissue development. The aim of this study was to investigate the effect of ghrelin on human embryonic stem cell (hESC) differentiation in infarcted cardiac microenvironment. The hESCs grown on feeder layers expressed several pluripotential markers including alkaline phosphatase (AKP). Four weeks after transplantation into rat infarcted hearts, the hESCs and their progeny cells survived and formed intracardiac grafts were 54.7% and 19.6% respectively in ghrelin- and phosphate-buffered saline (PBS)-treated groups. Double immunostaining with anti-human Sox9 and anti-HNA or anti-human fetal liver kinase-1 (Flk1) and anti beta-tubulin showed that the human grafts were in development. However, double positive stains were only found in the ghrelin-treated group. In addition, the hESC injection protocol was insufficient to restore heart function of the acute myocardial infarction model. Our study, therefore, provides a new insight of ghrelin on promoting hESC survival and differentiation in rat infarcted cardiac microenvironment. This may give a clue for therapy for myocardial infarction by hESCs or progeny cells. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000303181600014
项目编号30771032 ; 30700879 ; 81070701 ; 81000315 ; 2006AA02A112 ; 2012CB517502 ; 20100001110083
资助机构National Natural Sciences Foundation of China ; Chinese National 863 Program ; Chinese National 973 Program ; Specialized Research Fund for the Doctoral Program of Higher Education
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66482
专题北京大学第三临床医学院_生殖医学中心
北京大学第三临床医学院_内分泌科
作者单位1.Peking Univ, Hosp 3, Reprod Med Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Endocrinol & Metab, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Clin Stem Cell Res Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Gao, Meijuan,Yang, Jin,Liu, Guoqiang,et al. Ghrelin promotes the differentiation of human embryonic stem cells in infarcted cardiac microenvironment[J]. PEPTIDES,2012,34(2):373-379.
APA Gao, Meijuan.,Yang, Jin.,Liu, Guoqiang.,Wei, Rui.,Zhang, Lin.,...&Hong, Tianpei.(2012).Ghrelin promotes the differentiation of human embryonic stem cells in infarcted cardiac microenvironment.PEPTIDES,34(2),373-379.
MLA Gao, Meijuan,et al."Ghrelin promotes the differentiation of human embryonic stem cells in infarcted cardiac microenvironment".PEPTIDES 34.2(2012):373-379.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Gao, Meijuan]的文章
[Yang, Jin]的文章
[Liu, Guoqiang]的文章
百度学术
百度学术中相似的文章
[Gao, Meijuan]的文章
[Yang, Jin]的文章
[Liu, Guoqiang]的文章
必应学术
必应学术中相似的文章
[Gao, Meijuan]的文章
[Yang, Jin]的文章
[Liu, Guoqiang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。