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学科主题: 临床医学
题名:
Ghrelin promotes the differentiation of human embryonic stem cells in infarcted cardiac microenvironment
作者: Gao, Meijuan2,3; Yang, Jin2,3; Liu, Guoqiang2,3; Wei, Rui2,3; Zhang, Lin2,3; Wang, Haining2,3; Wang, Guang2,3; Gao, Hongwei2,3; Chen, Guian1,3; Hong, Tianpei2,3
关键词: Ghrelin ; Embryonic stem cells ; Differentiation ; Myocardial infarction
刊名: PEPTIDES
发表日期: 2012-04-01
DOI: 10.1016/j.peptides.2012.02.006
卷: 34, 期:2, 页:373-379
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]: MYOCARDIAL-INFARCTION ; ENDOTHELIAL-CELLS ; HEART ; PEPTIDE ; SOX9 ; RATS ; CARDIOMYOCYTES ; EXPRESSION ; SECRETION
英文摘要:

Ghrelin is broadly expressed in myocardial tissues, where it exerts different functions. It also has been found to have a wide variety of biological functions on cell differentiation and tissue development. The aim of this study was to investigate the effect of ghrelin on human embryonic stem cell (hESC) differentiation in infarcted cardiac microenvironment. The hESCs grown on feeder layers expressed several pluripotential markers including alkaline phosphatase (AKP). Four weeks after transplantation into rat infarcted hearts, the hESCs and their progeny cells survived and formed intracardiac grafts were 54.7% and 19.6% respectively in ghrelin- and phosphate-buffered saline (PBS)-treated groups. Double immunostaining with anti-human Sox9 and anti-HNA or anti-human fetal liver kinase-1 (Flk1) and anti beta-tubulin showed that the human grafts were in development. However, double positive stains were only found in the ghrelin-treated group. In addition, the hESC injection protocol was insufficient to restore heart function of the acute myocardial infarction model. Our study, therefore, provides a new insight of ghrelin on promoting hESC survival and differentiation in rat infarcted cardiac microenvironment. This may give a clue for therapy for myocardial infarction by hESCs or progeny cells. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.

语种: 英语
所属项目编号: 30771032 ; 30700879 ; 81070701 ; 81000315 ; 2006AA02A112 ; 2012CB517502 ; 20100001110083
项目资助者: National Natural Sciences Foundation of China ; Chinese National 863 Program ; Chinese National 973 Program ; Specialized Research Fund for the Doctoral Program of Higher Education
WOS记录号: WOS:000303181600014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66482
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Reprod Med Ctr, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Endocrinol & Metab, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Clin Stem Cell Res Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Gao, Meijuan,Yang, Jin,Liu, Guoqiang,et al. Ghrelin promotes the differentiation of human embryonic stem cells in infarcted cardiac microenvironment[J]. PEPTIDES,2012,34(2):373-379.
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