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学科主题: 临床医学
题名:
Rapid Identification of Pseudallescheria and Scedosporium Strains by Using Rolling Circle Amplification
作者: Lackner, Michaela1,2,3; Najafzadeh, Mohammad Javad4; Sun, Jiufeng1,5; Lu, Qiaoyun1,6; de Hoog, G. Sybren1,5,7
刊名: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
发表日期: 2012
DOI: 10.1128/AEM.05280-11
卷: 78, 期:1, 页:126-133
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]: BOYDII SPECIES COMPLEX ; PRACTICAL METHOD ; IN-VITRO ; FONSECAEA ; APIOSPERMUM ; ASPERGILLUS ; ECOLOGY ; MEMBERS ; NOV. ; PCR
英文摘要:

The Pseudallescheria boydii complex, comprising environmental pathogens with Scedosporium anamorphs, has recently been subdivided into five main species: Scedosporium dehoogii, S. aurantiacum, Pseudallescheria minutispora, P. apiosperma, and P. boydii, while the validity of some other taxa is being debated. Several Pseudallescheria and Scedosporium species are indicator organisms of pollution in soil and water. Scedosporium dehoogii in particular is enriched in soils contaminated by aliphatic hydrocarbons. In addition, the fungi may cause life-threatening infections involving the central nervous system in severely impaired patients. For screening purposes, rapid and economic tools for species recognition are needed. Our aim is to establish rolling circle amplification (RCA) as a screening tool for species-specific identification of Pseudallescheria and Scedosporium. With this aim, a set of padlock probes was designed on the basis of the internal transcribed spacer (ITS) region, differing by up to 13 fixed mutations. Padlock probes were unique as judged from sequence comparison by BLAST search in GenBank and in dedicated research databases at CBS (Centraalbureau voor Schimmelcultures Fungal Biodiversity Centre). RCA was applied as an in vitro tool, tested with pure DNA amplified from cultures. The species-specific padlock probes designed in this study yielded 100% specificity. The method presented here was found to be an attractive alternative to identification by restriction fragment length polymorphism (RFLP) or sequencing. The rapidity (<1 day), specificity, and low costs make RCA a promising screening tool for environmentally and clinically relevant fungi.

语种: 英语
项目资助者: Mashhad University of Medical Sciences, Mashhad, Iran
WOS记录号: WOS:000298396700017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66490
Appears in Collections:北京大学第一临床医学院_皮肤性病科_期刊论文

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作者单位: 1.Univ Innsbruck, Inst Microbiol, A-6020 Innsbruck, Austria
2.Mashhad Univ Med Sci, Dept Parasitol & Mycol, Fac Med, Mashhad, Iran
3.CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands
4.Fed Inst Drugs & Med Devices BfArM Biosafety Lab, Bonn, Germany
5.Sun Yat Sen Univ, Dept Dermatol, Affiliated Hosp 2, Guangzhou 510275, Guangdong, Peoples R China
6.Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Beijing 100871, Peoples R China
7.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands

Recommended Citation:
Lackner, Michaela,Najafzadeh, Mohammad Javad,Sun, Jiufeng,et al. Rapid Identification of Pseudallescheria and Scedosporium Strains by Using Rolling Circle Amplification[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2012,78(1):126-133.
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