IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Purification and characterization of 66-kDa glycoprotein from human breast carcinoma
Feng, Renqing; Liu, Xiang; Guo, Zhenquan; Li, Jinfeng
刊名CANCER SCIENCE
2007-09-01
DOI10.1111/j.1349-7006.2007.00539.x
98期:9页:1344-1349
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]TUMOR-ASSOCIATED ANTIGENS ; CANCER PATIENTS ; C-ERBB-2 ONCOPROTEIN ; PROGNOSTIC VALUE ; CEA ; TAMOXIFEN ; DIAGNOSIS ; MARKERS
英文摘要

We extracted a 66-kDa glycoprotein (GP-1D8) from breast invasive ductal carcinoma tissues. The monoclonal antibody (mAb) against GP-1D8 was prepared in our laboratory. Western blotting with the purified protein using the mAb demonstrated a single band of 66 kDa. Immunocytochemical and immunohistochemical analysis revealed strong expression of GP-1D8 protein in the cytoplasm of MCF-7 cells and different types of breast carcinoma tissues, but GP-1D8 is absent in normal breast and benign breast tumor tissues. Glycosylation analysis showed GP-1D8 contained methylated salic acid. GP-1D8 was identified using mass-spectrometric techniques and N-terminal sequencing. These data were used to identify the protein through the SWISSPROT protein sequence database and BLAST homology search. These results showed GP-1D8 had some similarity to human albumin precursor. Co-immunoprecipitation assays of lysate from MCF-7 cells and mass spectrometric analysis revealed the interaction of GP-1D8 with beta-tubulin. This is the first time human breast carcinoma tissues and MCF-7 cells have been shown to express a 66-kDa glycoprotein similar to human albumin precursor. These results might be important in the detection of novel potential biomarkers and may provide insight into the molecular mechanisms of tumorigenesis.

语种英语
WOS记录号WOS:000248968500010
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66511
专题北京大学临床肿瘤学院
作者单位1.Peking Univ, Coll Life Sci, Dept Biochem, Beijing 100871, Peoples R China
2.Peking Univ, Sch Oncol, Dept Surg, Beijing 100036, Peoples R China
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Feng, Renqing,Liu, Xiang,Guo, Zhenquan,et al. Purification and characterization of 66-kDa glycoprotein from human breast carcinoma[J]. CANCER SCIENCE,2007,98(9):1344-1349.
APA Feng, Renqing,Liu, Xiang,Guo, Zhenquan,&Li, Jinfeng.(2007).Purification and characterization of 66-kDa glycoprotein from human breast carcinoma.CANCER SCIENCE,98(9),1344-1349.
MLA Feng, Renqing,et al."Purification and characterization of 66-kDa glycoprotein from human breast carcinoma".CANCER SCIENCE 98.9(2007):1344-1349.
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