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学科主题临床医学
Rapid identification of clinical members of Fusarium fujikuroi complex using MALDI-TOF MS
Al-Hatmi, Abdullah M. S.1,2,3; Normand, Anne-Cecile4; van Diepeningen, Anne D.1; Hendrickx, Marijke5; de Hoog, G. Sybren1,2,6,7,8; Piarroux, Renaud4
关键词database fingerprint Fusarium fujikuroi complex MALDI-TOF MS multi-locus sequence analysis spectral analysis
刊名FUTURE MICROBIOLOGY
2015
DOI10.2217/fmb.15.108
10期:12页:1939-1952
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
研究领域[WOS]Microbiology
关键词[WOS]FLIGHT MASS-SPECTROMETRY ; DESORPTION IONIZATION-TIME ; SPECIES IDENTIFICATION ; FILAMENTOUS FUNGI ; MICROBIOLOGY ; INFECTIONS ; MYCOLOGY ; LEVEL
英文摘要

Aim: To develop the matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) method for identification of Fusarium species within Fusarium fujikuroi complex for use in clinical microbiology laboratories. Materials & methods: A total of 24 reference and 60 clinical and environmental isolates belonging to the F. fujikuroi complex were subjected to MALDI-TOF MS identification. Protein extracts of Fusarium isolates were obtained using formic acid extraction. Multilocus sequence analysis was used as a gold standard. Results: The MALDI-TOF MS Biotyper correctly identified 93.6% of the strains down to the species level, while the remaining isolates (6.4%) were identified at the genus level when using scores of 2.0 as cut-off values. Correct identification was obtained despite large intraspecific heterogeneities in MALDI-TOF spectra. Conclusion: MALDI-TOF MS is a rapid identification tool for the recognition of species within F. fujikuroi complex, provided a database is available.

语种英语
WOS记录号WOS:000365746600005
项目编号28/2014
资助机构Ministry of Health, Oman
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66512
专题北京大学第一临床医学院_皮肤性病科
作者单位1.CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands
2.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands
3.Ibri Hosp, Minist Hlth, Directorate Gen Hlth Serv, Ibri, Oman
4.Univ Aix Marseille 2, CHU Timone, Lab Parasitol Mycol, Marseille, France
5.BCCM IHEM Sci Inst Publ Hlth, Mycol Aerobiol Sect, Brussels, Belgium
6.Peking Univ, Hlth Sci Ctr, Res Ctr Med Mycol, Beijing 100871, Peoples R China
7.Second Mil Med Univ, Changzheng Hosp, Shanghai Inst Med Mycol, Shanghai, Peoples R China
8.King Abdulaziz Univ, Jeddah 21413, Saudi Arabia
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GB/T 7714
Al-Hatmi, Abdullah M. S.,Normand, Anne-Cecile,van Diepeningen, Anne D.,et al. Rapid identification of clinical members of Fusarium fujikuroi complex using MALDI-TOF MS[J]. FUTURE MICROBIOLOGY,2015,10(12):1939-1952.
APA Al-Hatmi, Abdullah M. S.,Normand, Anne-Cecile,van Diepeningen, Anne D.,Hendrickx, Marijke,de Hoog, G. Sybren,&Piarroux, Renaud.(2015).Rapid identification of clinical members of Fusarium fujikuroi complex using MALDI-TOF MS.FUTURE MICROBIOLOGY,10(12),1939-1952.
MLA Al-Hatmi, Abdullah M. S.,et al."Rapid identification of clinical members of Fusarium fujikuroi complex using MALDI-TOF MS".FUTURE MICROBIOLOGY 10.12(2015):1939-1952.
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