IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Involvement of Hepatopoietin Cn in the development of human hepatocellular carcinoma
Zhu, Bu-Dong1; Li, Xiao-Li2,3; Liu, Yong2; Chang, Jing2; Liu, Yang2; Zhang, Dong-Dong2; Wang, Qiang3; Ren, Jun1; Cui, Chun-Ping2
关键词Hepatopoietin Cn Hepatocellular carcinoma Cell proliferation Prognosis
刊名CLINICAL & EXPERIMENTAL METASTASIS
2010-12-01
DOI10.1007/s10585-010-9346-8
27期:8页:571-580
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]LEUCINE-RICH REPEATS ; GROWTH-FACTOR ; CELLS ; GENE ; IDENTIFICATION ; EXPRESSION ; APOPTOSIS ; PROGNOSIS ; FAMILY ; P53
英文摘要

Hepatopoietin Cn (HPPCn) is a novel nuclear protein with the ability to promote liver regeneration. In the present study, we investigated the expression profile of HPPCn and its functional activity in human hepatocellular carcinoma (HCC) cell line and tissue samples. HPPCn expression was detected in HCC cell lines and 54 paired HCC carcinomas by immunochemical staining and Western blotting. The functional activity of HPPCn in cell lines was evaluated by MTT and colony formation assays and with nude mouse model. The correlation of HPPCn expression with clinicopathological characteristics of 54 HCC patients was also analyzed. Our results showed that HPPCn protein was prominently located within the nuclei of hepatocytes and the expression level was evidently increased in HepG2 and Bel7402 cell lines compared with L02 normal hepatocytes. HPPCn silencing by small interfering RNA greatly suppressed HepG2 cell proliferation and colony formation capacity and the inhibitory effect was also observed in a Balb/c-null mouse model. The silencing HPPCn expression effectively enhanced the apoptosis of HepG2 cells. In addition, HPPCn expression was detected in 48 of 54 (89%) human HCC tissues in sharp contrast with the corresponding non-tumor liver tissues. HPPCn protein was mainly accumulated in the tumor nucleus. The elevated expression of HPPCn protein in tumors was significantly associated with poor tumor cellular differentiation and present of vascular invasion. Patients with higher HPPCn expression in tumors had significantly shorter overall survival (OS) of both all of patients and the patients at the early stage. On multivariate Cox analysis, elevated expression of HPPCn in tumors was found to be an independent prognostic factor for OS. Therefore, these data suggest that HPPCn expression might be involved in the development of HCC and could be served as a promising biomarker.

语种英语
WOS记录号WOS:000285242800003
项目编号30800558 ; 30930041 ; 2009ZX09103-617
资助机构National Natural Science Foundation of China ; Chinese major special S &amp ; T project for development of major new drug
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66519
专题北京大学临床肿瘤学院
北京大学临床肿瘤学院_乳腺肿瘤内科
作者单位1.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst,Dept Breast Oncol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
2.Beijing Inst Radiat Med, Dept Expt Hematol, Beijing 100850, Peoples R China
3.Beijing 309 Hosp, Beijing 100094, Peoples R China
推荐引用方式
GB/T 7714
Zhu, Bu-Dong,Li, Xiao-Li,Liu, Yong,et al. Involvement of Hepatopoietin Cn in the development of human hepatocellular carcinoma[J]. CLINICAL & EXPERIMENTAL METASTASIS,2010,27(8):571-580.
APA Zhu, Bu-Dong.,Li, Xiao-Li.,Liu, Yong.,Chang, Jing.,Liu, Yang.,...&Cui, Chun-Ping.(2010).Involvement of Hepatopoietin Cn in the development of human hepatocellular carcinoma.CLINICAL & EXPERIMENTAL METASTASIS,27(8),571-580.
MLA Zhu, Bu-Dong,et al."Involvement of Hepatopoietin Cn in the development of human hepatocellular carcinoma".CLINICAL & EXPERIMENTAL METASTASIS 27.8(2010):571-580.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhu, Bu-Dong]的文章
[Li, Xiao-Li]的文章
[Liu, Yong]的文章
百度学术
百度学术中相似的文章
[Zhu, Bu-Dong]的文章
[Li, Xiao-Li]的文章
[Liu, Yong]的文章
必应学术
必应学术中相似的文章
[Zhu, Bu-Dong]的文章
[Li, Xiao-Li]的文章
[Liu, Yong]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。