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学科主题: 临床医学
题名:
Involvement of Hepatopoietin Cn in the development of human hepatocellular carcinoma
作者: Zhu, Bu-Dong1; Li, Xiao-Li2,3; Liu, Yong2; Chang, Jing2; Liu, Yang2; Zhang, Dong-Dong2; Wang, Qiang3; Ren, Jun1; Cui, Chun-Ping2
关键词: Hepatopoietin Cn ; Hepatocellular carcinoma ; Cell proliferation ; Prognosis
刊名: CLINICAL & EXPERIMENTAL METASTASIS
发表日期: 2010-12-01
DOI: 10.1007/s10585-010-9346-8
卷: 27, 期:8, 页:571-580
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: LEUCINE-RICH REPEATS ; GROWTH-FACTOR ; CELLS ; GENE ; IDENTIFICATION ; EXPRESSION ; APOPTOSIS ; PROGNOSIS ; FAMILY ; P53
英文摘要:

Hepatopoietin Cn (HPPCn) is a novel nuclear protein with the ability to promote liver regeneration. In the present study, we investigated the expression profile of HPPCn and its functional activity in human hepatocellular carcinoma (HCC) cell line and tissue samples. HPPCn expression was detected in HCC cell lines and 54 paired HCC carcinomas by immunochemical staining and Western blotting. The functional activity of HPPCn in cell lines was evaluated by MTT and colony formation assays and with nude mouse model. The correlation of HPPCn expression with clinicopathological characteristics of 54 HCC patients was also analyzed. Our results showed that HPPCn protein was prominently located within the nuclei of hepatocytes and the expression level was evidently increased in HepG2 and Bel7402 cell lines compared with L02 normal hepatocytes. HPPCn silencing by small interfering RNA greatly suppressed HepG2 cell proliferation and colony formation capacity and the inhibitory effect was also observed in a Balb/c-null mouse model. The silencing HPPCn expression effectively enhanced the apoptosis of HepG2 cells. In addition, HPPCn expression was detected in 48 of 54 (89%) human HCC tissues in sharp contrast with the corresponding non-tumor liver tissues. HPPCn protein was mainly accumulated in the tumor nucleus. The elevated expression of HPPCn protein in tumors was significantly associated with poor tumor cellular differentiation and present of vascular invasion. Patients with higher HPPCn expression in tumors had significantly shorter overall survival (OS) of both all of patients and the patients at the early stage. On multivariate Cox analysis, elevated expression of HPPCn in tumors was found to be an independent prognostic factor for OS. Therefore, these data suggest that HPPCn expression might be involved in the development of HCC and could be served as a promising biomarker.

语种: 英语
所属项目编号: 30800558 ; 30930041 ; 2009ZX09103-617
项目资助者: National Natural Science Foundation of China ; Chinese major special S &amp ; T project for development of major new drug
WOS记录号: WOS:000285242800003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66519
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst,Dept Breast Oncol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
2.Beijing Inst Radiat Med, Dept Expt Hematol, Beijing 100850, Peoples R China
3.Beijing 309 Hosp, Beijing 100094, Peoples R China

Recommended Citation:
Zhu, Bu-Dong,Li, Xiao-Li,Liu, Yong,et al. Involvement of Hepatopoietin Cn in the development of human hepatocellular carcinoma[J]. CLINICAL & EXPERIMENTAL METASTASIS,2010,27(8):571-580.
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