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学科主题: 药学
题名:
gamma-Cyclodextrin-folate complex-functionalized quantum dots for tumor-targeting and site-specific labeling
作者: Zhao, Mei-Xia2; Huang, Hai-Feng1; Xia, Qing1; Ji, Liang-Nian2; Mao, Zong-Wan1,2
刊名: JOURNAL OF MATERIALS CHEMISTRY
发表日期: 2011
DOI: 10.1039/c1jm11066g
卷: 21, 期:28, 页:10290-10297
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Physical ; Materials Science, Multidisciplinary
研究领域[WOS]: Chemistry ; Materials Science
关键词[WOS]: CDSE/CDS CORE/SHELL NANOCRYSTALS ; BETA-CYCLODEXTRIN ; LIVING CELLS ; NANOPARTICLES ; RECEPTOR ; CANCER ; DELIVERY ; DRUG ; PROBES ; LUMINESCENCE
英文摘要:

Quantum dots (QDs) encapsulated with high affinity ligands, specifically binding organic ligands, such as antibodies, peptides or small molecules, have been used in intracellular imaging and targeting. In this work, a series of folate-receptor targeted QDs, in which tumor-targeting folic acid (FA) was conjugated to the surface of QDs through cell-penetrated gamma-cyclodextrin (gamma-CD), was synthesized. The QDs showed good optical properties and biocompatibility, such as strong optical emission, long luminescent lifetime, appropriate size (4-5 nm in diameter), and lower toxicity. In particular, the gamma-CD-FA-coated CdSe-ZnSe QDs presented lower cytotoxicity to these cells at higher IC(50) concentrations of above 200 mu g mL(-1) for 48 h. Folate-receptor overexpressed FR(+) and folate-receptor deficient FR(-) cells were incubated with folate-receptor targeted gamma-CD-FA-coated QDs and non-targeted L-Cys-beta-CD-coated QDs in vitro. It was found that folate-receptor targeted QDs could more effectively recognize cancer cells with folate receptor (FR) over-expression compared to non-targeted QDs by flow cytometry and confocal laser scanning microscopy (CLSM). Confirming the specificity of folate-receptor targeted QDs, binding and internalization were inhibited by free folate, and hardly any uptake was found in FR (+) cells. Inside the cells, the gamma-CD-FA-functionalized QDs are mostly distributed within lysosomes. These properties not only offer insights into the mechanism of the functionalized QDs delivery but also will guide the design and development of nanoparticle probes for intracellular imaging and targeting applications.

语种: 英语
所属项目编号: 20725103 ; 20831006 ; 20821001 ; 20901005 ; 9351027501000003 ; 2007CB815306
项目资助者: National Natural Science Foundation of China ; Guangdong Provincial Natural Science Foundation ; National Basic Research Program of China (973 Program) ; Fundamental Research Funds for the Central Universities
WOS记录号: WOS:000292982800012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66542
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, State Key Lab Nat & Biomimet Drug, Beijing 100191, Peoples R China
2.Sun Yat Sen Univ, Sch Chem & Chem Engn, MOE Key Lab Bioinorgan & Synthet Chem, Guangzhou 510275, Guangdong, Peoples R China

Recommended Citation:
Zhao, Mei-Xia,Huang, Hai-Feng,Xia, Qing,et al. gamma-Cyclodextrin-folate complex-functionalized quantum dots for tumor-targeting and site-specific labeling[J]. JOURNAL OF MATERIALS CHEMISTRY,2011,21(28):10290-10297.
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