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学科主题: 口腔医学
题名:
Magnetic bead-based salivary peptidome profiling for periodontal-orthodontic treatment
作者: Zhang, Jieni1,2; Zhou, Shaonan1,2; Li, Ruoxuan3; Cao, Tian1,2; Zheng, Hui1,2; Wang, Xuedong1,2; Zhou, Yanheng1,2; Du, Ning1; Chen, Feng1; Lin, Jiuxiang1,2
关键词: Periodontal-orthodontic treatment ; Peptidome ; Saliva ; MALDI-TOF MS
刊名: PROTEOME SCIENCE
发表日期: 2012-11-06
DOI: 10.1186/1477-5956-10-63
卷: 10
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: BREAST-CANCER PATIENTS ; BIOMARKER DISCOVERY ; THROMBOMODULIN ; INFLAMMATION ; PROTEOMICS ; OBESITY ; CELLS ; STAGE
英文摘要:

Background: Patients with periodontitis seek periodontal-orthodontic treatment to address certain functional and aesthetic problems. However, little is known of the effect of periodontitis on orthodontic treatment. Thus, we compared the differences in peptide mass fingerprints of orthodontic patients with and without periodontitis by MALDI-TOF MS using a magnetic bead-based peptidome analysis of saliva samples. In this way, we aimed to identify and explore a panel of differentially-expressed specific peptides.

Results: Saliva samples from 24 patients (eight orthodontic patients without periodontitis, eight with periodontitis and another eight with periodontitis but no orthodontic treatment) were analyzed, and peptide mass fingerprints were created by scanning MS signals using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with magnetic beads. Nine mass peaks showed significant differences. Orthodontic patients in the group without periodontal disease showed higher mass peaks for seven peptides of the nine, whereas the mass peaks for the other two peptides were higher in the periodontal-orthodontic patients. Besides, these differentially-expressed peptides were sequenced.

Conclusions: The elucidated candidate biomarkers indicated interactions between periodontal condition and orthodontic treatment and their contributions to the changes of saliva protein profiles. Our results provide novel insight into the altered salivary protein profile during periodontal-orthodontic treatment, and may lead to the development of a therapeutic monitoring strategy for periodontics and orthodontics.

语种: 英语
所属项目编号: PKUSS20110301
项目资助者: Peking University School of Stomatology
WOS记录号: WOS:000311968100001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66546
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch Stomatol, Dept Cent Lab, Beijing 100081, Peoples R China
2.Peking Univ, Sch Stomatol, Dept Orthodont, Beijing 100081, Peoples R China
3.Capital Univ Med Sci, Dept Stomatol, Beijing An Zhen Hosp, Beijing 100081, Peoples R China

Recommended Citation:
Zhang, Jieni,Zhou, Shaonan,Li, Ruoxuan,et al. Magnetic bead-based salivary peptidome profiling for periodontal-orthodontic treatment[J]. PROTEOME SCIENCE,2012,10.
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