IR@PKUHSC  > 北京大学第一临床医学院  > 肾脏内科
学科主题临床医学
Effects of exogenous homocysteine on cellular proliferation and extracellular matrix production in cultured rat mesangial cells
Li, J; Li, XM; Wang, HY
关键词cell homocysteine mesangium
刊名NEPHROLOGY
2001-08-01
6期:4页:173-178
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]INDEPENDENT RISK FACTOR ; SMOOTH-MUSCLE CELLS ; ATHEROGENIC STIMULUS ; ENDOTHELIAL-CELLS ; VASCULAR-DISEASE ; ATHEROSCLEROSIS ; EXPRESSION ; HYPERHOMOCYSTEINEMIA ; ACTIVATION ; GROWTH
英文摘要

Homocysteine (HCY) is a thiol-containing amino acid produced as a result of the demethylation of methionine. It has been proved that elevated HCY is associated with a high risk of arteriosclerotic cardiovascular disease. Although hyperhomocysteinaemia is observed frequently in patients with end-stage renal disease, its role in the process of glomerulosclerosis is not clear. This study investigated the effects of exogenous HCY on proliferation, phenotype change and extracellular matrix production in rat mesangial cells (MCs). The results revealed that DNA synthesis in MCs, measured by the [H-3]-thymidine uptake, did not increase significantly when the cells were treated with HCY at concentrations of 5 x 10(-5), 5 x 10(-4) or 1 x 10(-3) mol/L, even though thymidine incorporation in MCs increased two- to fourfold using 20% fetal calf serum-RPMI medium or platelet-derived growth factor (20 ng/mL). A dose-dependent increase in thymidine incorporation in vascular smooth muscle cells was also found when these cells were treated with HCY at similar concentrations (P < 0.01). The cell cycle was not changed when MCs were stimulated by HCY at different doses (5 x 10(-5), 5 x 10(-4) and 1 x 10(-3) mol/L) or at different time points (24, 48 and 72 h). Additionally, increased extracellular signal-regulated kinase was observed in MCs induced by PDGF (10 ng/mL), but not by HCY (0.5 mmol/L) for 2-60 min. alpha -Smooth muscle actin, detected using Western blot analysis, was not changed when MCs were exposed to 0.5 mmol/L HCY for 6, 12, 18, 24 and 36 h. Fibronectin and laminin, which are detected in the supernatant of cultured MCs by inhibition enzyme-linked immunosorbent assay, were not changed when MCs were exposed to HCY (5 x 10(-4) mol/L) for 2, 4, 6 or 8 days. These results suggest that HCY has no effect on proliferation, phenotype change or extracellular matrix production in MCs. This indicates that HCY may not be a key factor contributing to the process of glomerulosclerosis.

语种英语
WOS记录号WOS:000171609400005
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66552
专题北京大学第一临床医学院_肾脏内科
作者单位Beijing Univ, Hosp 1, Inst Nephrol, Dept Med,Renal Div, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Li, J,Li, XM,Wang, HY. Effects of exogenous homocysteine on cellular proliferation and extracellular matrix production in cultured rat mesangial cells[J]. NEPHROLOGY,2001,6(4):173-178.
APA Li, J,Li, XM,&Wang, HY.(2001).Effects of exogenous homocysteine on cellular proliferation and extracellular matrix production in cultured rat mesangial cells.NEPHROLOGY,6(4),173-178.
MLA Li, J,et al."Effects of exogenous homocysteine on cellular proliferation and extracellular matrix production in cultured rat mesangial cells".NEPHROLOGY 6.4(2001):173-178.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, J]的文章
[Li, XM]的文章
[Wang, HY]的文章
百度学术
百度学术中相似的文章
[Li, J]的文章
[Li, XM]的文章
[Wang, HY]的文章
必应学术
必应学术中相似的文章
[Li, J]的文章
[Li, XM]的文章
[Wang, HY]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。