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学科主题: 临床医学
题名:
Effects of exogenous homocysteine on cellular proliferation and extracellular matrix production in cultured rat mesangial cells
作者: Li, J; Li, XM; Wang, HY
关键词: cell ; homocysteine ; mesangium
刊名: NEPHROLOGY
发表日期: 2001-08-01
卷: 6, 期:4, 页:173-178
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: INDEPENDENT RISK FACTOR ; SMOOTH-MUSCLE CELLS ; ATHEROGENIC STIMULUS ; ENDOTHELIAL-CELLS ; VASCULAR-DISEASE ; ATHEROSCLEROSIS ; EXPRESSION ; HYPERHOMOCYSTEINEMIA ; ACTIVATION ; GROWTH
英文摘要:

Homocysteine (HCY) is a thiol-containing amino acid produced as a result of the demethylation of methionine. It has been proved that elevated HCY is associated with a high risk of arteriosclerotic cardiovascular disease. Although hyperhomocysteinaemia is observed frequently in patients with end-stage renal disease, its role in the process of glomerulosclerosis is not clear. This study investigated the effects of exogenous HCY on proliferation, phenotype change and extracellular matrix production in rat mesangial cells (MCs). The results revealed that DNA synthesis in MCs, measured by the [H-3]-thymidine uptake, did not increase significantly when the cells were treated with HCY at concentrations of 5 x 10(-5), 5 x 10(-4) or 1 x 10(-3) mol/L, even though thymidine incorporation in MCs increased two- to fourfold using 20% fetal calf serum-RPMI medium or platelet-derived growth factor (20 ng/mL). A dose-dependent increase in thymidine incorporation in vascular smooth muscle cells was also found when these cells were treated with HCY at similar concentrations (P < 0.01). The cell cycle was not changed when MCs were stimulated by HCY at different doses (5 x 10(-5), 5 x 10(-4) and 1 x 10(-3) mol/L) or at different time points (24, 48 and 72 h). Additionally, increased extracellular signal-regulated kinase was observed in MCs induced by PDGF (10 ng/mL), but not by HCY (0.5 mmol/L) for 2-60 min. alpha -Smooth muscle actin, detected using Western blot analysis, was not changed when MCs were exposed to 0.5 mmol/L HCY for 6, 12, 18, 24 and 36 h. Fibronectin and laminin, which are detected in the supernatant of cultured MCs by inhibition enzyme-linked immunosorbent assay, were not changed when MCs were exposed to HCY (5 x 10(-4) mol/L) for 2, 4, 6 or 8 days. These results suggest that HCY has no effect on proliferation, phenotype change or extracellular matrix production in MCs. This indicates that HCY may not be a key factor contributing to the process of glomerulosclerosis.

语种: 英语
WOS记录号: WOS:000171609400005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66552
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

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作者单位: Beijing Univ, Hosp 1, Inst Nephrol, Dept Med,Renal Div, Beijing 100034, Peoples R China

Recommended Citation:
Li, J,Li, XM,Wang, HY. Effects of exogenous homocysteine on cellular proliferation and extracellular matrix production in cultured rat mesangial cells[J]. NEPHROLOGY,2001,6(4):173-178.
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