IR@PKUHSC  > 北京大学基础医学院  > 心血管所
学科主题基础医学
Generation of a gene knockout mouse by using a gene trap strategy
Tan, XH; Cheng, X; Mao, CM; Chen, GH; Yang, X
关键词gene trap mouse gene knockout
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2004-07-01
31期:7页:606-610
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]EMBRYONIC STEM-CELLS ; MICE
英文摘要

A gene trap construct was used to transfect mouse ES cells. 36 ES colonies trapped by one copy of neo gene were obtained. The beta-galactosidase activity was detectable in 14 ES colonies. ES cells from 3 trapped lines were introduced into blastocysts by microinjection. Two chimeric mice lines were generated. One trapped mutation went through the germline. Genomic sequences adjacent to integration sites of the constructs were isolated by plasmid rescue. The results of sequencing suggested that the trapped gene is possibly a novel gene. The expression pattern of this gene shown by beta-galactosidase expression was restricted in abdomen and lib bud of E10.5 mouse embryo.

语种中文
WOS记录号WOS:000222914400006
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66567
专题北京大学基础医学院_心血管所
作者单位Peking Univ, Inst Cardiovasc Sci, Beijing 100083, Peoples R China
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GB/T 7714
Tan, XH,Cheng, X,Mao, CM,et al. Generation of a gene knockout mouse by using a gene trap strategy[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2004,31(7):606-610.
APA Tan, XH,Cheng, X,Mao, CM,Chen, GH,&Yang, X.(2004).Generation of a gene knockout mouse by using a gene trap strategy.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,31(7),606-610.
MLA Tan, XH,et al."Generation of a gene knockout mouse by using a gene trap strategy".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 31.7(2004):606-610.
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