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学科主题: 基础医学
题名:
Generation of a gene knockout mouse by using a gene trap strategy
作者: Tan, XH; Cheng, X; Mao, CM; Chen, GH; Yang, X
关键词: gene trap ; mouse ; gene knockout
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2004-07-01
卷: 31, 期:7, 页:606-610
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: EMBRYONIC STEM-CELLS ; MICE
英文摘要:

A gene trap construct was used to transfect mouse ES cells. 36 ES colonies trapped by one copy of neo gene were obtained. The beta-galactosidase activity was detectable in 14 ES colonies. ES cells from 3 trapped lines were introduced into blastocysts by microinjection. Two chimeric mice lines were generated. One trapped mutation went through the germline. Genomic sequences adjacent to integration sites of the constructs were isolated by plasmid rescue. The results of sequencing suggested that the trapped gene is possibly a novel gene. The expression pattern of this gene shown by beta-galactosidase expression was restricted in abdomen and lib bud of E10.5 mouse embryo.

语种: 中文
WOS记录号: WOS:000222914400006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66567
Appears in Collections:基础医学院_心血管所_期刊论文

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作者单位: Peking Univ, Inst Cardiovasc Sci, Beijing 100083, Peoples R China

Recommended Citation:
Tan, XH,Cheng, X,Mao, CM,et al. Generation of a gene knockout mouse by using a gene trap strategy[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2004,31(7):606-610.
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