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学科主题基础医学
Direct coupling between arachidonic acid-induced Ca2+ release and Ca2+ entry in HEK293 cells
Luo, DL; Sun, HL; Lan, XM; Xiao, RP; Han, Q
关键词arachidonic acid Ca2+ release Ca2+ entry Gd3+ coupling isotetrandrine
刊名PROSTAGLANDINS & OTHER LIPID MEDIATORS
2005
DOI10.1016/j.prostaglandins.2004.11.004
75期:1-4页:141-151
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]VASCULAR SMOOTH-MUSCLE ; UNSATURATED FATTY-ACIDS ; ENDOTHELIAL-CELLS ; CALCIUM-RELEASE ; MEMBRANE-FLUIDITY ; ACTIVATION ; MOBILIZATION ; PATHWAYS ; INFLUX ; STORE
英文摘要

Arachidonic acid (AA) modulates intracellular Ca2+ signaling via Ca2+ release or/and Ca2+ entry. However, the mechanism underlies either process is unknown; nor is it clear as to whether the two processes are mechanistically linked. By using Fura2/AM, we found that AA induced mobilization of internal Ca2+ store and an increment in Ca2+, Mn2+ and Ba2+ influx in HEK293 cells. The AA-mediated Ca2+ signaling was not due to AA metabolites, and insensitive to capacitative Ca2+ entry inhibitors. Interestingly, isotetrandrine and Gd3+ inhibited both AA-induced Ca2+ release and Ca2+ entry in a concentration-dependent manner without affecting Ca2+ discharge caused by carbachol, caffeine, or thapsigargin. Additionally, similar pattern of inhibition was observed with tetracaine treatment. More importantly, the three compounds exhibited almost equal potent inhibition of AA-initiated Ca2+ release as well as Ca2+ influx. Therefore, this study, for the first time, provides evidence for a direct coupling between AA-mediated Ca2+ release and Ca2+ entry. (c) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000227735900011
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66576
Collection北京大学基础医学院_心血管所
作者单位1.Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci, Beijing 100083, Peoples R China
2.NIA, Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA
Recommended Citation
GB/T 7714
Luo, DL,Sun, HL,Lan, XM,et al. Direct coupling between arachidonic acid-induced Ca2+ release and Ca2+ entry in HEK293 cells[J]. PROSTAGLANDINS & OTHER LIPID MEDIATORS,2005,75(1-4):141-151.
APA Luo, DL,Sun, HL,Lan, XM,Xiao, RP,&Han, Q.(2005).Direct coupling between arachidonic acid-induced Ca2+ release and Ca2+ entry in HEK293 cells.PROSTAGLANDINS & OTHER LIPID MEDIATORS,75(1-4),141-151.
MLA Luo, DL,et al."Direct coupling between arachidonic acid-induced Ca2+ release and Ca2+ entry in HEK293 cells".PROSTAGLANDINS & OTHER LIPID MEDIATORS 75.1-4(2005):141-151.
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