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学科主题: 基础医学
题名:
Direct coupling between arachidonic acid-induced Ca2+ release and Ca2+ entry in HEK293 cells
作者: Luo, DL; Sun, HL; Lan, XM; Xiao, RP; Han, Q
关键词: arachidonic acid ; Ca2+ release ; Ca2+ entry ; Gd3+ ; coupling ; isotetrandrine
刊名: PROSTAGLANDINS & OTHER LIPID MEDIATORS
发表日期: 2005
DOI: 10.1016/j.prostaglandins.2004.11.004
卷: 75, 期:1-4, 页:141-151
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: VASCULAR SMOOTH-MUSCLE ; UNSATURATED FATTY-ACIDS ; ENDOTHELIAL-CELLS ; CALCIUM-RELEASE ; MEMBRANE-FLUIDITY ; ACTIVATION ; MOBILIZATION ; PATHWAYS ; INFLUX ; STORE
英文摘要:

Arachidonic acid (AA) modulates intracellular Ca2+ signaling via Ca2+ release or/and Ca2+ entry. However, the mechanism underlies either process is unknown; nor is it clear as to whether the two processes are mechanistically linked. By using Fura2/AM, we found that AA induced mobilization of internal Ca2+ store and an increment in Ca2+, Mn2+ and Ba2+ influx in HEK293 cells. The AA-mediated Ca2+ signaling was not due to AA metabolites, and insensitive to capacitative Ca2+ entry inhibitors. Interestingly, isotetrandrine and Gd3+ inhibited both AA-induced Ca2+ release and Ca2+ entry in a concentration-dependent manner without affecting Ca2+ discharge caused by carbachol, caffeine, or thapsigargin. Additionally, similar pattern of inhibition was observed with tetracaine treatment. More importantly, the three compounds exhibited almost equal potent inhibition of AA-initiated Ca2+ release as well as Ca2+ influx. Therefore, this study, for the first time, provides evidence for a direct coupling between AA-mediated Ca2+ release and Ca2+ entry. (c) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000227735900011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66576
Appears in Collections:基础医学院_心血管所_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci, Beijing 100083, Peoples R China
2.NIA, Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA

Recommended Citation:
Luo, DL,Sun, HL,Lan, XM,et al. Direct coupling between arachidonic acid-induced Ca2+ release and Ca2+ entry in HEK293 cells[J]. PROSTAGLANDINS & OTHER LIPID MEDIATORS,2005,75(1-4):141-151.
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