IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Expression of cystic fibrosis transmembrane conductance regulator in human endometrium
Zheng, XY; Chen, GA; Wang, HY
关键词CFTR chloride channel human endometrium menstrual cycle
刊名HUMAN REPRODUCTION
2004-12-01
DOI10.1093/humrep/deh507
19期:12页:2933-2941
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]COMPETITIVE RT-PCR ; CFTR MESSENGER-RNA ; EPITHELIAL-CELLS ; PROSTAGLANDIN E-2 ; SWEAT GLAND ; GENE ; SECRETION ; LOCALIZATION ; WOMEN ; FLUID
英文摘要

Background: As a cAMP-regulated Cl- channel, cystic fibrosis transmembrane conductance regulator (CFTR) plays a critical role in the active secretion of electrolytes and fluid in epithelial cells. Women with CFTR gene mutations are less fertile, generally assumed to be due to cervical factors. However, there is little known about CFTR protein expression in human endometrium and its possible roles in reproduction. Methods and Results: CFTR protein and mRNA levels in human endometrium were analysed using immunohistochemical and in situ hybridization methods, respectively. Significant expression of CFTR protein was only seen in the glandular cells from late proliferative to all secretory phases, consistent with western blot analysis. High levels of CFTR mRNA were present only around the ovulatory period. In cultured glandular cells, the production of CFTR protein and mRNA was stimulated by estradiol and inhibited by progesterone. A forskolin-activated Cl- current in endometrial epithelial cells with a linear I-V relationship was detected by the whole-cell patch-clamp technique. Conclusions: (i) CFTR mRNA and protein were localized in human endometrial epithelial cells and the amounts varied in a cyclic manner; (ii) CFTR expression in cultured glandular cells was up- and downregulated by estradiol and progesterone, respectively; and (iii) CFTR in human endometrium functions as a cAMP-activated Cl- channel.

语种英语
WOS记录号WOS:000225254300036
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66594
专题北京大学第三临床医学院
作者单位Peking Univ, Hosp 3, Dept Obstet & Gynaecol, Beijing 100083, Peoples R China
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Zheng, XY,Chen, GA,Wang, HY. Expression of cystic fibrosis transmembrane conductance regulator in human endometrium[J]. HUMAN REPRODUCTION,2004,19(12):2933-2941.
APA Zheng, XY,Chen, GA,&Wang, HY.(2004).Expression of cystic fibrosis transmembrane conductance regulator in human endometrium.HUMAN REPRODUCTION,19(12),2933-2941.
MLA Zheng, XY,et al."Expression of cystic fibrosis transmembrane conductance regulator in human endometrium".HUMAN REPRODUCTION 19.12(2004):2933-2941.
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