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学科主题: 临床医学
题名:
C-reactive protein enhances the respiratory burst of neutrophils-induced by antineutrophil cytoplasmic antibody
作者: Xu, Peng-cheng1,2,3,4,5; Hao, Jian1,2,3,4; Yang, Xiao-wei1,2,3,4; Chang, Dong-yuan1,2,3,4; Chen, Min1,2,3,4; Zhao, Ming-hui1,2,3,4
关键词: C-reactive protein ; Neutrophil ; Anti-neutrophil cytoplasmic antibody ; Vasculitis
刊名: MOLECULAR IMMUNOLOGY
发表日期: 2012-10-01
DOI: 10.1016/j.molimm.2012.05.012
卷: 52, 期:3-4, 页:148-154
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Immunology
研究领域[WOS]: Biochemistry & Molecular Biology ; Immunology
关键词[WOS]: SERUM-AMYLOID-P ; AORTIC ENDOTHELIAL-CELLS ; APOPTOTIC CELLS ; COMPONENT BINDS ; PENTRAXIN PTX3 ; ACTIVATION ; RISK ; GRANULOMATOSIS ; AUTOANTIBODIES ; REARRANGEMENT
英文摘要:

Serum C-reactive protein (CRP) was one of the useful biomarkers for evaluating the disease activity in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). Cumulating studies proved that CRP was pathogenic in a variety of diseases. In the current study, the in vitro effects of CRP to prime neutrophils for ANCA-induced respiratory burst were investigated with flow cytometry. Without TNF-alpha in the reactive system, ANCA could only induce a slight level of respiratory burst of neutrophils. CRP could enhance the respiratory burst of neutrophils induced by ANCA against myeloperoxidse [mean fluorescence intensity (MFI, 68.45 +/- 16.87 vs. 58.65 +/- 15.09, P<0.05) or by ANCA against proteinase 3 (MFI, 79.51 +/- 15.90 vs. 61.73 +/- 14.89, P<0.05). Although CRP (50 mu g/mL, incubating for 30 min) could not active neutrophils alone, after incubation with neutrophils for 10 min, CRP (50 mu g/mL) could increase the expression of membrane proteinase 3 of neutrophils (MFI, 365.27 +/- 143.50 vs. 235.32 +/- 124.65, P<0.05). Heat-treated CRP could not enhance the levels of neutrophils respiratory burst induced by ANCA or increase the expression of membrane proteinase 3 of neutrophils. So CRP can prime neutrophils and enhance the respiratory burst induced by ANCA and might be pathogenic in AAV. (C) 2012 Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 2012CB517702 ; 30972733 ; 81021004
项目资助者: Chinese 973 project ; National Natural Science Fund
WOS记录号: WOS:000307489800007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66608
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Med, Div Renal, Beijing 100034, Peoples R China
2.Peking Univ, Inst Nephrol, Beijing 100034, Peoples R China
3.Minist Hlth China, Key Lab Renal Dis, Beijing 100034, Peoples R China
4.Minist Educ China, Key Lab CKD Prevent & Treatment, Beijing 100034, Peoples R China
5.Tianjin Med Univ, Gen Hosp, Dept Nephrol, Tianjin 300052, Peoples R China

Recommended Citation:
Xu, Peng-cheng,Hao, Jian,Yang, Xiao-wei,et al. C-reactive protein enhances the respiratory burst of neutrophils-induced by antineutrophil cytoplasmic antibody[J]. MOLECULAR IMMUNOLOGY,2012,52(3-4):148-154.
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