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学科主题临床医学
Sustained norepinephrine stimulation induces different regulation of expression in three alpha(1)-adrenoceptor subtypes
Lei, BL; Zhang, YY; Han, C
关键词alpha 1-adrenoceptor human embryonic kidney cells regulation signal transduction
刊名LIFE SCIENCES
2001-06-08
69期:3页:301-308
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]RECEPTOR SUBTYPES ; DOWN-REGULATION ; AGONIST ; KINASE ; DESENSITIZATION
英文摘要

The norepinephrine (NE) -induced regulation of alpha (1)-adrenoceptors (ARs) expression in human embryonic kidney (HEK) 293 cells stably expressing cloned alpha (1)-AR subtypes with similar receptor densities was investigated. In the presence of 10 muM propranolol,the treatment of cells with 10 muM NE for 4-72 h down-regulated alpha (1A)- and alpha (1D)-AR, but increased alpha (1B)-AR expression in a time-dependent manner. The down-regulation of alpha (1A)-AR reached maximum of 40.3 +/- 14.7 % at 48h. The down-regulation of alpha (1D)-AR reached maximum of 51.3 +/- 3.7 % at 24h. With the stimulation of NE, alpha (1B)-AR density was increased maximally by 112.4 +/- 43.4 % at 48h. The protein kinase C (PKC) inhibitor calphostin C or Ro-31-8220 abolished the NE-induced down-regulation of alpha (1A)- and alpha (1D)-AR, but showed no effect on the up-regulation of alpha (1B)-AR. The PKC agonist PMA not only mimicked the NE-induced down-regulation of alpha (1A)- and alpha (1D)-AR, but also induced a down-regulation of alpha (1B)-AR. The endoplasmic reticulum Ca2+-ATPase inhibitor cyclopiazonic acid (CPA) or thapsigargin, or the calcium chelator BAPTA/AM did not affect the down-regulation of alpha (1A)-AR, but inhibited the up-regulation of alpha (1B)-AR induced by NE. Calmodulin antagonist W-7, tyrosine kinase inhibitor genistein or tyrphostin A25 had no effect on NE-induced up-regulation of alpha (1B)-AR. The results suggest that three alpha (1)-AR subtypes ale differently regulated by sustained NE stimulation with different signal transduction pathways. (C) 2001 Elsevier Science Inc. All rights reserved.

语种英语
WOS记录号WOS:000169214500007
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66610
专题北京大学第三临床医学院_心血管内科
作者单位Beijing Med Univ, Third Hosp, Inst Vasc Med, Beijing 100083, Peoples R China
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GB/T 7714
Lei, BL,Zhang, YY,Han, C. Sustained norepinephrine stimulation induces different regulation of expression in three alpha(1)-adrenoceptor subtypes[J]. LIFE SCIENCES,2001,69(3):301-308.
APA Lei, BL,Zhang, YY,&Han, C.(2001).Sustained norepinephrine stimulation induces different regulation of expression in three alpha(1)-adrenoceptor subtypes.LIFE SCIENCES,69(3),301-308.
MLA Lei, BL,et al."Sustained norepinephrine stimulation induces different regulation of expression in three alpha(1)-adrenoceptor subtypes".LIFE SCIENCES 69.3(2001):301-308.
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