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学科主题: 临床医学
题名:
An Anti-DLK1 Monoclonal Antibody Produced Using ELISA and Hybridoma Techniques
作者: Jin, Zhong-hui1; Zhao, Li-zhe2; Zhang, Yanyan1; Zhang, Weifang1
刊名: HYBRIDOMA
发表日期: 2009-12-01
DOI: 10.1089/hyb.2009.0043
卷: 28, 期:6, 页:441-445
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]: HOMEOTIC PROTEIN DLK ; DELTA-LIKE DLK ; GENE ; EXPRESSION ; CARCINOMA ; GROWTH ; DIFFERENTIATION ; TUMORS ; CELLS ; GTL2
英文摘要:

DLK1 is a newly identified prognostic factor associated with liver cancer survival. To prepare specific monoclonal antibody (MAb) against DLK1, cDNA of DLK1 was cloned by RT-PCR and inserted into prokaryotic expression vector pGEX-4T1, respectively. The fusion proteins were expressed in Escherichia coli. Monoclonal antibody against DLK1 was obtained with hybridoma technique and specific ELISA screening. Western blotting and immunohistochemistry assays showed that MAb 6D6 had specific binding ability with DLK1 protein in eukaryotic cells and cancer tissues. This MAb will be a helpful tool for the detection of DLK1 protein in the tissues and serum of liver cancer and other cancer patients.

语种: 英语
WOS记录号: WOS:000272955100007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66611
Appears in Collections:北京大学第三临床医学院_核医学科_期刊论文

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作者单位: 1.BioSino Biotechnol & Sci Inc, Beijing, Peoples R China
2.Peking Univ, Dept Nucl Med, Hosp 3, Beijing 100191, Peoples R China

Recommended Citation:
Jin, Zhong-hui,Zhao, Li-zhe,Zhang, Yanyan,et al. An Anti-DLK1 Monoclonal Antibody Produced Using ELISA and Hybridoma Techniques[J]. HYBRIDOMA,2009,28(6):441-445.
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