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学科主题: 药学
题名:
Studies on cultivated Ephedra plants in inner Mongolia autonomous region and Ningxia Hui autonomous region
作者: Kakiuchi, N; Nakajima, I; Kurita, Y; Long, CF; Cai, SQ; Mikage, M
关键词: Ephedra species ; cultivation ; nuclear ribosomal DNA (nrDNA) ; internal transcribing sequence (ITS) ; chloroplastic DNA
刊名: BIOLOGICAL & PHARMACEUTICAL BULLETIN
发表日期: 2006-04-01
卷: 29, 期:4, 页:746-749
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: MITOCHONDRIAL
英文摘要:

Progression of the desertification in northern China has been causing damage to wild Ephedra plants on which we depend for most of supply of the traditional herbal medicine, "Ma huang." The Chinese government encourages the cultivation of Ephedra plants, and Ephedra fields have been reclaimed in the original Ephedra habitats in recent years. We surveyed 7 Ephedra fields that have been recently developed in the Inner Mongolia Autonomous Region and Ningxia Hui Autonomous Region to collect information on Ephedra plant cultivation, especially pertaining to crop species. Specimens taken from those Ephedra fields were genetically and morphologically analyzed, and their ephedrine alkaloid content was examined. DNA analyses of Ephedra specimens, including DNA sequencing of ITS (internal transcribing sequence of nuclear ribosomal DNA) and trn LIF (intron of trnL and intergenic spacer between the trnL and trnF of chloroplast DNA) region and species-specific amplification of trn LIF were conducted to identify Ephedra species. Based on the results of DNA sequencing and morphological determination, the crops grown in 6 fields ware identified as Ephedra sinica, while co-planting of E. sinica and E. intermedia was found in one field where a higher appearance rate of plants with varied morphology from wild Ephedra plants was observed. Furthermore, direct sequencing of the PCR product of the trn LIF region of some specimens from the field and their species-specific PCR showed ambivalent result. Cloning and sequencing of the PCR product of the trn LIF region of those specimens DNA suggested their heteroplasmy, containing both E. sinica- and E. intermedia-type chloroplasts. On the other hand, the profile of the ephedrine alkaloid content was clearly correlated with the result of direct sequencing of the trn LIF region; the specimens showing the E. sinica-type sequence contained more ephedrine than pseudoephedrine, and the specimens of the E. intermedia-type more pseudoephedrine.

语种: 英语
WOS记录号: WOS:000237310700026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/66617
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Kanazawa Univ, Grad Sch Nat Sci & Technol, Kanazawa, Ishikawa 9201192, Japan
2.Beijing Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Kakiuchi, N,Nakajima, I,Kurita, Y,et al. Studies on cultivated Ephedra plants in inner Mongolia autonomous region and Ningxia Hui autonomous region[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2006,29(4):746-749.
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