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Studies on cultivated Ephedra plants in inner Mongolia autonomous region and Ningxia Hui autonomous region
Kakiuchi, N; Nakajima, I; Kurita, Y; Long, CF; Cai, SQ; Mikage, M
关键词Ephedra species cultivation nuclear ribosomal DNA (nrDNA) internal transcribing sequence (ITS) chloroplastic DNA
刊名BIOLOGICAL & PHARMACEUTICAL BULLETIN
2006-04-01
29期:4页:746-749
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]MITOCHONDRIAL
英文摘要

Progression of the desertification in northern China has been causing damage to wild Ephedra plants on which we depend for most of supply of the traditional herbal medicine, "Ma huang." The Chinese government encourages the cultivation of Ephedra plants, and Ephedra fields have been reclaimed in the original Ephedra habitats in recent years. We surveyed 7 Ephedra fields that have been recently developed in the Inner Mongolia Autonomous Region and Ningxia Hui Autonomous Region to collect information on Ephedra plant cultivation, especially pertaining to crop species. Specimens taken from those Ephedra fields were genetically and morphologically analyzed, and their ephedrine alkaloid content was examined. DNA analyses of Ephedra specimens, including DNA sequencing of ITS (internal transcribing sequence of nuclear ribosomal DNA) and trn LIF (intron of trnL and intergenic spacer between the trnL and trnF of chloroplast DNA) region and species-specific amplification of trn LIF were conducted to identify Ephedra species. Based on the results of DNA sequencing and morphological determination, the crops grown in 6 fields ware identified as Ephedra sinica, while co-planting of E. sinica and E. intermedia was found in one field where a higher appearance rate of plants with varied morphology from wild Ephedra plants was observed. Furthermore, direct sequencing of the PCR product of the trn LIF region of some specimens from the field and their species-specific PCR showed ambivalent result. Cloning and sequencing of the PCR product of the trn LIF region of those specimens DNA suggested their heteroplasmy, containing both E. sinica- and E. intermedia-type chloroplasts. On the other hand, the profile of the ephedrine alkaloid content was clearly correlated with the result of direct sequencing of the trn LIF region; the specimens showing the E. sinica-type sequence contained more ephedrine than pseudoephedrine, and the specimens of the E. intermedia-type more pseudoephedrine.

语种英语
WOS记录号WOS:000237310700026
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66617
专题北京大学药学院
作者单位1.Kanazawa Univ, Grad Sch Nat Sci & Technol, Kanazawa, Ishikawa 9201192, Japan
2.Beijing Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Kakiuchi, N,Nakajima, I,Kurita, Y,et al. Studies on cultivated Ephedra plants in inner Mongolia autonomous region and Ningxia Hui autonomous region[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2006,29(4):746-749.
APA Kakiuchi, N,Nakajima, I,Kurita, Y,Long, CF,Cai, SQ,&Mikage, M.(2006).Studies on cultivated Ephedra plants in inner Mongolia autonomous region and Ningxia Hui autonomous region.BIOLOGICAL & PHARMACEUTICAL BULLETIN,29(4),746-749.
MLA Kakiuchi, N,et al."Studies on cultivated Ephedra plants in inner Mongolia autonomous region and Ningxia Hui autonomous region".BIOLOGICAL & PHARMACEUTICAL BULLETIN 29.4(2006):746-749.
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