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学科主题临床医学
Differentiation of rabbit bone marrow mesenchymal stem cells into corneal epithelial cells in vivo and ex vivo
Gu, Shaofeng4; Xing, Chengzhong3; Han, Jingyi4; Tso, Mark O. M.1,2; Hong, Jing1
刊名MOLECULAR VISION
2009-01-16
15期:9-11页:99-107
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Ophthalmology
资助者National Natural Science Fund of P. R. China ; Shenyang Science Technology Fund ; National Natural Science Fund of P. R. China ; Shenyang Science Technology Fund
研究领域[WOS]Biochemistry & Molecular Biology ; Ophthalmology
关键词[WOS]STROMAL CELLS ; AMNIOTIC MEMBRANE ; PROGENITOR CELLS ; EXPRESSION ; KERATIN ; TRANSPLANTATION ; RECONSTRUCTION ; ENGRAFTMENT ; PHENOTYPE ; SURFACE
英文摘要

Purpose: To examine whether bone marrow mesenchymal stem cells (MSCs) could be differentiated into corneal epithelial cells in vivo and ex vivo.

Methods: In vivo, BrdU labeled rabbit MSCs (Rb-MSCs) were suspended in the fibrin gels and transplanted onto the surface of the damaged rabbit corneas. Histology and molecular phenotype were studied on postoperative day 28. In vitro, labeled Rb-MSCs were cultured for three days in two different systems: (1) Group A: Rb-MSCs were co-cultured with rabbit limbal stem cells (Rb-LSCs) by the Transwell culture system. A suspension of Rb-LSCs was added to the upper membrane surface, and the inserts were positioned in the culture wells, which were incubated with Rb-MSCs; (2) Group B: Supernatant medium that had first been used to culture Rb-LSCs and then filtered with a 0.45 mu m filter was used to culture Rb-MSCs. For both groups, immunofluorescence and flow cytometric analysis were used to examine the expression of cytokeratin 3 (CK3) in differentiated Rb-MSCs.

Results: In vivo, the data showed that following transplantation of Rb-MSCs, the rabbit′s damaged corneal surface was successfully reconstructed and that some Rb-MSCs participated in the healing of the injured corneal epithelium and expressed CK3. In vitro, the data showed that Rb-MSCs rapidly differentiated into cells with a morphological and molecular phenotype of corneal epithelial-like cells. For both groups, the differentiated Rb-MSCs were positive for corneal epithelial-specific marker CK3. In Group A, flow cytometry analysis showed that at day one, only 3.46 +/- 1.9% of cells expressed CK3. This increased to 7.24 +/- 3.80% at day two and decreased slightly (5.50 +/- 3.33%) at day three. The proportion of CK3 in Group B was 4.09 +/- 1.84% at day one, rising to 9.31 +/- 5.92% after 24 h, but falling (4.37 +/- 2.61%) at day three. The mean differences are significant between each group and the negative control, but was not significant between Group A and Group B.

Conclusions: MSCs could differentiate into corneal epithelial-like cells in vivo and ex vivo.

语种英语
所属项目编号30670816 ; 30471865 ; 1053111-3-07
资助者National Natural Science Fund of P. R. China ; Shenyang Science Technology Fund ; National Natural Science Fund of P. R. China ; Shenyang Science Technology Fund
WOS记录号WOS:000264363800002
引用统计
被引频次:67[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/66619
专题北京大学第三临床医学院_眼科
作者单位1.Peking Univ Third Hosp, Peking Univ Eye Ctr, Beijing 100083, Peoples R China
2.Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21205 USA
3.China Med Univ, Affiliated Hosp 1, Dept Oncol, Shenyang, Peoples R China
4.China Med Univ, Affiliated Hosp 1, Dept Ophthalmol, Shenyang, Peoples R China
推荐引用方式
GB/T 7714
Gu, Shaofeng,Xing, Chengzhong,Han, Jingyi,et al. Differentiation of rabbit bone marrow mesenchymal stem cells into corneal epithelial cells in vivo and ex vivo[J]. MOLECULAR VISION,2009,15(9-11):99-107.
APA Gu, Shaofeng,Xing, Chengzhong,Han, Jingyi,Tso, Mark O. M.,&Hong, Jing.(2009).Differentiation of rabbit bone marrow mesenchymal stem cells into corneal epithelial cells in vivo and ex vivo.MOLECULAR VISION,15(9-11),99-107.
MLA Gu, Shaofeng,et al."Differentiation of rabbit bone marrow mesenchymal stem cells into corneal epithelial cells in vivo and ex vivo".MOLECULAR VISION 15.9-11(2009):99-107.
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